Results 91 to 100 of about 631,742 (318)

Random-sequence genetic oligomer pools display an innate potential for ligation and recombination

eLife, 2018
Recombination, the exchange of information between different genetic polymer strands, is of fundamental importance in biology for genome maintenance and genetic diversification and is mediated by dedicated recombinase enzymes. Here, we describe an innate
Hannes Mutschler, Alexander I Taylor, Benjamin T Porebski, Alice Lightowlers, Gillian Houlihan, Mikhail Abramov, Piet Herdewijn, Philipp Holliger   +7 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Single nucleus sequencing reveals evidence of inter-nucleus recombination in arbuscular mycorrhizal fungi

eLife, 2018
Eukaryotes thought to have evolved clonally for millions of years are referred to as ancient asexuals. The oldest group among these are the arbuscular mycorrhizal fungi (AMF), which are plant symbionts harboring hundreds of nuclei within one continuous ...
Eric CH Chen, Stephanie Mathieu, Anne Hoffrichter, Kinga Sedzielewska-Toro, Max Peart, Adrian Pelin, Steve Ndikumana, Jeanne Ropars, Steven Dreissig, Jorg Fuchs, Andreas Brachmann, Nicolas Corradi   +11 more
doaj   +1 more source

Impact of DNA ligase IV on nonhomologous end joining pathways during class switch recombination in human cells

, 2005
Class switch recombination (CSR) is a region-specific, transcriptionally regulated, nonhomologous recombinational process that is initiated by activation-induced cytidine deaminase (AID).
Lennart Hammarström, Hammarström, L,, Lahdesmaki, A, Aleksi Lähdesmäki, Wei Zhou, Andrew R. Gennery, Jones, AM, Michael R. Ehrenstein, Pan-Hammarstrom, Q, Hammarstrom, L, Zhou, W, Gennery, AR, Ehrenstein, MR, Anne-Marie Jones, Richard A. Gatti, Gatti, RA, Qiang Pan-Hammarström   +16 more
core   +1 more source

Aging Is a Key Driver for Adult Acute Myeloid Leukemia

Aging and Cancer, EarlyView.
Acute myeloid leukemia (AML) is a classical age‐related hematologic malignancy, and a key driver of AML is aging, which profoundly regulates intrinsic factors such as genomic instability, epigenetic reprogramming, and metabolic dysregulation, and alters bone marrow microenvironment.
Rong Yin, Haojian Zhang
wiley   +1 more source

Crystal structure of Hop2-Mnd1 and mechanistic insights into its role in meiotic recombination [PDF]

, 2015
In meiotic DNA recombination, the Hop2-Mnd1 complex promotes Dmc1-mediated single-stranded DNA (ssDNA) invasion into homologous chromosomes to form a synaptic complex by a yet-unclear mechanism.
Dal Peraro, Matteo, Dal Peraro, M., Kalantzi, Alexandra-Styliani, Toseland, Christopher P., Oh, B., Shin, H.-C., Oh, Byung-Ha, M D Peraro, A S Kalantzi, Toseland, C.P., Shin, Ho-Chul, S Gruber, Kalantzi, A., Kang, H.-A., Kang, H., Toseland, C., Ho-Chul Shin, Gruber, Stephan, Kalantzi, A.-S., Oh, B.-H., Kim, H.-M., H A Kang, B H Oh, Kang, Hyun-Ah, Kim, Hyun-Min, Gruber, S., H M Kim, Shin, H., C P Toseland, Kim, H.   +29 more
core   +1 more source

Influence of an Argon/Silane Atmosphere on the Temperature of a Thermal Plasma

Advanced Engineering Materials, EarlyView.
The influence of a silane‐doped argon atmosphere on the chemical composition and temperature of a thermal nontransferring argon plasma is investigated using optical emission spectroscopy. As a result of the high amount of free electrons resulting from the stepwise ionization and dissociation of the silane molecule, even a silane addition of 0.01 vol ...
Lena Kreie, David Kramer, Thomas Scheithauer, Hans Jürgen Maier, Thomas Hassel   +4 more
wiley   +1 more source

Nonthermal Plasma Reduction of Nickel Oxide Layers by Atmospheric Pressure Dielectric Barrier Discharge in Argon/Hydrogen

Advanced Engineering Materials, EarlyView.
This work explores the reduction of nickel oxide layers using dielectric barrier discharge plasma in an argon–hydrogen atmosphere. The results reveal a strong temperature dependence, enabling complete reduction of thick oxide layers at 200°C within seconds.
Maria Argirusi, Viktor Udachin, Sascha Jan Zimmermann, Alexander Tillmanns, Lienhard Wegewitz, Wolfgang Maus‐Friedrichs   +5 more
wiley   +1 more source

Exploring Protein Sequence Space Using Computationally Directed Recombination [PDF]

, 2006
Evolution has provided us with many protein sequences. However, these sequences represent a very small fraction of the possible sequences. In the laboratory, scientists have explored areas of sequence space not represented by natural proteins both to ...
Meyer, Michelle Margaret
core   +1 more source

Optimization of the Production of Rubber Compounds Using Mathematical Models

Advanced Engineering Materials, EarlyView.
Rubber compounds were mixed in a batch internal mixer, and symbolic regression was used to derive mathematical models linking recipe and process parameters to ram path, torque, and mixing quality (incorporation, dispersion, distribution). Subsequent optimization with evolutionary algorithms identified operating conditions that reduce specific energy ...
Anke Bardehle, Andreas Limper, Ulrich Giese, Carlos Lucke, Mario Beiner, Christoph Gögelein, Lisa Leuchtenberger‐Engel, Christian Hopmann   +7 more
wiley   +1 more source