Results 11 to 20 of about 530,778 (295)

A forest is more than its trees: haplotypes and ancestral recombination graphs. [PDF]

Genetics
Fritze H, Pope N, Kelleher J, Ralph P.
europepmc   +3 more sources

Highly Expressed Soluble Recombinant Anti-GFP VHHs in Escherichia coli via Optimized Signal Peptides, Strains, and Inducers

Frontiers in Molecular Biosciences, 2022
Antigen-binding variable domains of the H chain of heavy-chain antibodies (VHHs), also known as nanobodies (Nbs), are of great interest in imaging technique, disease prevention, diagnosis, and therapy.
Shuangying Chao, Shuangying Chao, Yuhang Liu, Yuhang Liu, Ning Ding, Ning Ding, Yue Lin, Yue Lin, Qian Wang, Qian Wang, Junwen Tan, Junwen Tan, Wei Li, Wei Li, Yang Zheng, Yang Zheng, Xuejun Hu, Xuejun Hu, Junming Li   +18 more
doaj   +1 more source

GEN1 promotes common fragile site expression

Cell Reports, 2023
Summary: Our genomes harbor conserved DNA sequences, known as common fragile sites (CFSs), that are difficult to replicate and correspond to regions of genome instability.
Anaid Benitez, Marie Sebald, Radhakrishnan Kanagaraj, Monica C. Rodrigo-Brenni, Ying Wai Chan, Chih-Chao Liang, Stephen C. West   +6 more
doaj   +1 more source

Plasma Membrane Anchoring and Gag:Gag Multimerization on Viral RNA Are Critical Properties of HIV-1 Gag Required To Mediate Efficient Genome Packaging

mBio, 2021
Human immunodeficiency virus type 1 (HIV-1) Gag selects and packages the HIV RNA genome during virus assembly. However, HIV-1 RNA constitutes only a small fraction of the cellular RNA.
Alice Duchon, Steven Santos, Jianbo Chen, Matthew Brown, Olga A. Nikolaitchik, Sheldon Tai, Jeffrey A. Chao, Eric O. Freed, Vinay K. Pathak, Wei-Shau Hu   +9 more
doaj   +1 more source

Coding and noncoding transcriptomes of NODULIN HOMEOBOX (NDX)-deficient Arabidopsis inflorescence

Scientific Data, 2023
Arabidopsis NODULIN HOMEOBOX (NDX) is a plant-specific transcriptional regulator whose role in small RNA biogenesis and heterochromatin homeostasis has recently been described. Here we extend our previous transcriptomic analysis to the flowering stage of
Orsolya Feró, Zsolt Karányi, Éva Nagy, Ágnes Mosolygó-L, Henrik Mihály Szaker, Tibor Csorba, Lóránt Székvölgyi   +6 more
doaj   +1 more source

Adaptation of HIV-1/HIV-2 Chimeras with Defects in Genome Packaging and Viral Replication

mBio, 2022
Frequent recombination is a hallmark of retrovirus replication. In rare cases, recombination occurs between distantly related retroviruses, generating novel viruses that may significantly impact viral evolution and public health.
Jonathan M. O. Rawson, Olga A. Nikolaitchik, Jennifer A. Yoo, Xayathed Somoulay, Matthew A. Brown, Franck S. Mbuntcha Bogni, Vinay K. Pathak, Ferri Soheilian, Ryan L. Slack, Stefan G. Sarafianos, Wei-Shau Hu   +10 more
doaj   +1 more source

Development of a Cell-Based Luciferase Complementation Assay for Identification of SARS-CoV-2 3CL^pro Inhibitors

Viruses, 2021
The 3C-like protease (3CLpro) of SARS-CoV-2 is considered an excellent target for COVID-19 antiviral drug development because it is essential for viral replication and has a cleavage specificity distinct from human proteases.
Jonathan M. O. Rawson, Alice Duchon, Olga A. Nikolaitchik, Vinay K. Pathak, Wei-Shau Hu   +4 more
doaj   +1 more source

NODULIN HOMEOBOX is required for heterochromatin homeostasis in Arabidopsis

Nature Communications, 2022
Arabidopsis NDX was previously reported as a regulator of euchromatic gene expression. Here the authors show that NDX functions at pericentromeric regions and regulates heterochromatin homeostasis by controlling siRNA production and non-CG methylation.
Zsolt Karányi, Ágnes Mosolygó-L, Orsolya Feró, Adrienn Horváth, Beáta Boros-Oláh, Éva Nagy, Szabolcs Hetey, Imre Holb, Henrik Mihály Szaker, Márton Miskei, Tibor Csorba, Lóránt Székvölgyi   +11 more
doaj   +1 more source

Evolution of the recombination regulator PRDM9 in minke whales

BMC Genomics, 2022
Background PRDM9 is a key regulator of meiotic recombination in most metazoans, responsible for reshuffling parental genomes. During meiosis, the PRDM9 protein recognizes and binds specific target motifs via its array of C2H2 zinc-fingers encoded by a ...
Elena Damm, Kristian K. Ullrich, William B. Amos, Linda Odenthal-Hesse   +3 more
doaj   +1 more source

Transcription Start Site Heterogeneity and Preferential Packaging of Specific Full-Length RNA Species Are Conserved Features of Primate Lentiviruses

Microbiology Spectrum, 2022
HIV-1 must package its RNA genome to generate infectious viruses. Recent studies have revealed that during genome packaging, HIV-1 not only excludes cellular mRNAs, but also distinguishes among full-length viral RNAs. Using NL4-3 and MAL molecular clones,
Jonathan M. O. Rawson, Olga A. Nikolaitchik, Saurabh Shakya, Brandon F. Keele, Vinay K. Pathak, Wei-Shau Hu   +5 more
doaj   +1 more source