Results 1 to 10 of about 871 (166)

A new insight into RecA filament regulation by RecX from the analysis of conformation-specific interactions [PDF]

eLife, 2022
RecA protein mediates homologous recombination repair in bacteria through assembly of long helical filaments on ssDNA in an ATP-dependent manner.
Aleksandr Alekseev, Georgii Pobegalov, Natalia Morozova, Alexey Vedyaykin, Galina Cherevatenko, Alexander Yakimov, Dmitry Baitin, Mikhail Khodorkovskii   +7 more
doaj   +4 more sources

A Robust One-Step Recombineering System for Enterohemorrhagic Escherichia coli [PDF]

Microorganisms, 2022
Enterohemorrhagic Escherichia coli (EHEC) can cause severe diarrheic in humans. To improve therapy options, a better understanding of EHEC pathogenicity is essential. The genetic manipulation of EHEC with classical one-step methods, such as the transient
Lang Peng, Rexford Mawunyo Dumevi, Marco Chitto, Nadja Haarmann, Petya Berger, Gerald Koudelka, Herbert Schmidt, Alexander Mellmann, Ulrich Dobrindt, Michael Berger   +9 more
doaj   +2 more sources

CRISPR-based genome editing reveals the roles of efflux pumps in <i>Mycobacterium abscessus</i>. [PDF]

mLife
Abstract Mycobacterium abscessus, one of the most antimicrobial‐resistant bacteria, is increasingly recognized as the cause of infections that are difficult to treat. Novel genetic manipulation tools are required to elucidate the biology, pathogenesis, and antibiotic resistance mechanisms of M. abscessus.
Li S, Duan A, Zhang L, Wang C, Yan M, Ren GX, Pan LP, Sun YC.   +7 more
europepmc   +2 more sources

DdiA, an XRE family transcriptional regulator, is a co-regulator of the DNA damage response in Myxococcus xanthus [PDF]

Journal of Bacteriology
Repair of DNA damage is essential for genome integrity. DNA damage elicits a DNA damage response (DDR) that includes error-free and error-prone, i.e., mutagenic, repair. The SOS response is a widely conserved system in bacteria that regulates the DDR and
Jana Jung, Timo Glatter, Marco Herfurth, Lotte Søgaard-Andersen   +3 more
doaj   +2 more sources

Effects of common Gram-negative pathogens causing male genitourinary-tract infections on human sperm functions [PDF]

Scientific Reports, 2021
Male genitourinary tract (MGT) bacterial infections are considered responsible for 15% of male infertility, but the mechanisms underlying decreased semen quality are poorly known. We evaluated in vitro the effect of strains of Gram-negative uropathogenic
Sara Marchiani, Ilaria Baccani, Lara Tamburrino, Giorgio Mattiuz, Sabrina Nicolò, Chiara Bonaiuto, Carmen Panico, Linda Vignozzi, Alberto Antonelli, Gianmaria Rossolini, Maria Torcia, Elisabetta Baldi   +11 more
doaj   +2 more sources

A genome-wide CRISPRi screen identifies homologous recombination pathway as potential target for broad-spectrum antibiotic adjuvants [PDF]

Journal of Advanced Research
Introduction: The widespread misuse and overuse of antibiotics have driven the emergence of multidrug-resistant and pan drug-resistant bacteria, constituting a formidable global health threat. Antibiotic adjuvants that potentiate the efficacy of existing
Haijie Zhang, Bo Chen, Lina Gu, Chenlong Wang, Lei Xu, Xinyuan Ji, Jie Wang, Zhiqiang Wang, Xia Xiao, Yuan Liu   +9 more
doaj   +2 more sources

Programmable Base Editing in Mycobacterium tuberculosis Using an Engineered CRISPR RNA-Guided Cytidine Deaminase [PDF]

Frontiers in Genome Editing, 2021
Multidrug-resistant Mycobacterium tuberculosis (Mtb) infection seriously endangers global human health, creating an urgent need for new treatment strategies.
Xin-Yuan Ding, Si-Shang Li, Yi-Man Geng, Mei-Yi Yan, Guo-Bao Li, Guo-Liang Zhang, Yi-Cheng Sun   +6 more
doaj   +2 more sources

A CRISPR-nonhomologous end-joining-based strategy for rapid and efficient gene disruption in <i>Mycobacterium abscessus</i>. [PDF]

mLife
Abstract Mycobacterium abscessus, a fast‐growing, non‐tuberculous mycobacterium resistant to most antimicrobial drugs, causes a wide range of serious infections in humans, posing a significant public health challenge. The development of effective genetic manipulation tools for M.
Zeng S, Ju Y, Alam MS, Lu Z, Hameed HMA, Li L, Tian X, Fang C, Fang X, Ding J, Wang X, Hu J, Wang S, Zhang T.   +13 more
europepmc   +2 more sources

Characterization and Fine Mapping of ds, a Recessive Dense-Spike Mutant Associated with Shortened Spike Axis and Increased Spikelet Number in Wheat [PDF]

Plants
Spike density is an important component of wheat spike architecture and is determined by the combined effects of spike-axis elongation and spikelet number.
Xiangtai Che, Zhuo Li, Shaoyuan Chen, Luxue Liu, Jie Ning, Jinwei Feng, Xin Wang, Yanhu Guo, Haotong Sun, Qingquan Chen, Jiancheng Song, Jing Zhao, Lei Chen   +12 more
doaj   +2 more sources

A droplet digital PCR assay to measure pilin antigenic variation frequency in Neisseria gonorrhoeae [PDF]

mSphere
The strict human pathogen Neisseria gonorrhoeae (gonococcus [Gc]) infects an estimated 82 million individuals globally and is a World Health Organization-designated bacterial pathogen of public health importance due to escalating antimicrobial resistance.
Sarah J. Quillin, Di Luo, Aoife Gavagan, Arthur Prindle, H Steven Seifert   +4 more
doaj   +2 more sources