Results 41 to 50 of about 436 (127)
Production of Monoclonal Antibodies against Red Sea Bream Iridovirus.
A total of 20 hybridoma clones secreting monoclonal antibodies (MAbs) were established between mouse myeloma cells and spleen cells from mice immunized with red sea bream iridovirus (RSIV) isolated from diseased red sea bream Pagrus major. These MAbs reacted with RSIV-infected BF-2 cells but not with uninfected BF-2 cells in immunofluorescence assays ...
Nakajima, Kazuhiro, Sorimachi, Minoru
openaire +2 more sources
Iridovirus Infection of Cultured Red Sea Bream, Pagrus major.
1990年秋に初めて発生し, 1991年に西日本の主要養殖県に発生域が拡大した養殖マダイの大量斃死の原因究明を行った結果, 以下のことが明らかとなった。 1.病魚の外観は体色の黒化や褪色, 体表や鰭の出血性のスレを特徴とし, 剖検すると鯉の褪色, 鰓弁の点状出血や先端部からの出血, 囲心腔内の出血等が観察された。 2.病理組織学的には脾臓組織のほか, 心臓の心内膜下, 鰓の中心静脈洞内皮および中肋の軟骨膜に接する組織間隙, 腎臓の間質組織と糸球体に肥大球形化した細胞が多数観察されるのが特徴であった。肝臓ではその出現頻度は低かった。 3.電顕観察ではこれらの異形肥大細胞の細胞質内に平面的には6角形を呈する, 直径が200~240nmのウイルス粒子の増殖像が観察された ...
Inouye, Kiyoshi +6 more
openaire +1 more source
SUSCEPTIBILITY OF DIFFERENT CELLS TO RED SEA BREAM IRIDOVIRUS (RSIV)
RSIV is an isolate virus in the genus Megalocytivirus (family Iridoviridae) that has been reported to be pathogen in more than 31 marine fish species in East Asia. The aim of study was to know the susceptibility of several cultured cells to RSIV. RSIV inoculum was inoculated onto cultured cells and then incubated in 25oC.
Ketut Mahardika, Indah Mastuti
openaire +2 more sources
Antigen Analysis of Red Sea Bream Iridovirus and Comparison with other Fish Iridoviruses.
Red sea bream iridovirus (RSIV) causes epizootic disease outbreaks involving mortality in cultured marine fishes in southwestern Japan. The reactivities of anti-RSIV polyclonal antibody and the monoclonal antibodies (MAbs) against RSIV to the RSIV isolates from various cultured marine fish species and diverse geographic regions were examined.
Nakajima, Kazuhiro +4 more
openaire +2 more sources
ABSTRACT Large‐scale double‐digest RAD sequencing (ddRADseq) datasets generated for genotyping are increasingly available in aquaculture, yet their unmapped reads remain largely unexplored for pathogen surveillance. Here, we evaluated the utility and limitations of repurposing unmapped ddRADseq reads to examine pathogen‐associated and disease ...
Nguyen Thanh Vu +11 more
wiley +1 more source
Lymphocystis Disease in Endemic Aotearoa New Zealand Bully (Gobiomorphus cotidianus)
ABSTRACT We detected lymphocystis disease virus (LCDV) for the first time in an Aotearoa New Zealand freshwater environment, from an individual common bully, toitoi, Gobiomorphus cotidianus (Eleotridae). Dinah's bully, Gobiomorphus dinae, and Kaharore bully, Gobiomorphus mataraerore, also exhibited gross nodular lesions on the fins but were not sampled.
Kate S. Hutson +10 more
wiley +1 more source
ABSTRACT Formalin‐inactivated vaccines are widely employed as a primary preventive strategy against red sea bream iridoviral disease (RSIVD), which poses a substantial economic threat to the aquaculture of rock bream (Oplegnathus fasciatus). However, conventional quantitative PCR (qPCR) cannot differentiate infectious virions from noninfectious vaccine
Sung‐Bin Moon +8 more
wiley +1 more source
ABSTRACT Spring viremia of carp virus (SVCV) is a contagious pathogen associated with significant mortality and economic losses in freshwater aquaculture. Although reverse transcription quantitative PCR (RT‐qPCR) enables rapid detection, it does not distinguish between infectious and non‐infectious viral particles, which may lead to overestimation of ...
Ha‐Jeong Son +9 more
wiley +1 more source
ABSTRACT The rapid expansion of cage culture for Nile tilapia (Oreochromis niloticus) in Lake Victoria is central to food security and profitable development in East Africa. Nevertheless, viral conditions affect the sustainability of this sector, with significant imbalances in livelihood, trade and aquaculture adaptability.
Richard S. Komba, Chacha J. Mwita
wiley +1 more source
Stress Nutrition in Aquatic Animals: From Definition to Practice
ABSTRACT Aquaculture faces numerous challenges, with stress being one of the major issues that lead to growth loss, metabolic disorders, weakened immunity, redox imbalance, and organ damage in aquatic animals. Nutritional intervention is one of the effective strategies to address these problems. Traditional research has primarily focused on the impacts
Jian Zhang +8 more
wiley +1 more source

