Results 41 to 50 of about 458 (125)
Iridovirus Infection of Cultured Red Sea Bream, Pagrus major.
1990年秋に初めて発生し, 1991年に西日本の主要養殖県に発生域が拡大した養殖マダイの大量斃死の原因究明を行った結果, 以下のことが明らかとなった。 1.病魚の外観は体色の黒化や褪色, 体表や鰭の出血性のスレを特徴とし, 剖検すると鯉の褪色, 鰓弁の点状出血や先端部からの出血, 囲心腔内の出血等が観察された。 2.病理組織学的には脾臓組織のほか, 心臓の心内膜下, 鰓の中心静脈洞内皮および中肋の軟骨膜に接する組織間隙, 腎臓の間質組織と糸球体に肥大球形化した細胞が多数観察されるのが特徴であった。肝臓ではその出現頻度は低かった。 3.電顕観察ではこれらの異形肥大細胞の細胞質内に平面的には6角形を呈する, 直径が200~240nmのウイルス粒子の増殖像が観察された ...
Inouye, Kiyoshi +6 more
openaire +1 more source
SUSCEPTIBILITY OF DIFFERENT CELLS TO RED SEA BREAM IRIDOVIRUS (RSIV)
RSIV is an isolate virus in the genus Megalocytivirus (family Iridoviridae) that has been reported to be pathogen in more than 31 marine fish species in East Asia. The aim of study was to know the susceptibility of several cultured cells to RSIV. RSIV inoculum was inoculated onto cultured cells and then incubated in 25oC.
Ketut Mahardika, Indah Mastuti
openaire +2 more sources
Production of Monoclonal Antibodies against Red Sea Bream Iridovirus.
A total of 20 hybridoma clones secreting monoclonal antibodies (MAbs) were established between mouse myeloma cells and spleen cells from mice immunized with red sea bream iridovirus (RSIV) isolated from diseased red sea bream Pagrus major. These MAbs reacted with RSIV-infected BF-2 cells but not with uninfected BF-2 cells in immunofluorescence assays ...
Nakajima, Kazuhiro, Sorimachi, Minoru
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Antigen Analysis of Red Sea Bream Iridovirus and Comparison with other Fish Iridoviruses.
Red sea bream iridovirus (RSIV) causes epizootic disease outbreaks involving mortality in cultured marine fishes in southwestern Japan. The reactivities of anti-RSIV polyclonal antibody and the monoclonal antibodies (MAbs) against RSIV to the RSIV isolates from various cultured marine fish species and diverse geographic regions were examined.
Nakajima, Kazuhiro +4 more
openaire +2 more sources
ABSTRACT The rapid expansion of cage culture for Nile tilapia (Oreochromis niloticus) in Lake Victoria is central to food security and profitable development in East Africa. Nevertheless, viral conditions affect the sustainability of this sector, with significant imbalances in livelihood, trade and aquaculture adaptability.
Richard S. Komba, Chacha J. Mwita
wiley +1 more source
Stress Nutrition in Aquatic Animals: From Definition to Practice
ABSTRACT Aquaculture faces numerous challenges, with stress being one of the major issues that lead to growth loss, metabolic disorders, weakened immunity, redox imbalance, and organ damage in aquatic animals. Nutritional intervention is one of the effective strategies to address these problems. Traditional research has primarily focused on the impacts
Jian Zhang +8 more
wiley +1 more source
Global Warming Affects the Pathogenesis of Important Fish Diseases in European Aquaculture
The effect of global warming on pathogens and their fish hosts that could exacerbate the negative outcomes for aquaculture. Changes in farming practices and the development of innovative mitigation tools may prove essential to cope with the effects and impacts of rising water temperatures on fish diseases in Europe.
George Rigos +7 more
wiley +1 more source
Abstract Infectious diseases impact numerous organisms. Knowledge of host‐pathogen interactions and host responses to infection is crucial for conservation and management. Obtaining this knowledge quickly is made increasingly possible by a variety of genomic approaches, yet, for many species the bottleneck to understanding this, remains access to ...
Allison K. Miller +7 more
wiley +1 more source
Polymerase Chain Reaction (PCR) Amplification of DNA of Red Sea Bream Iridovirus (RSIV)
The polymerase chain reaction (PCR) was used to amplify DNA of the red sea bream iridovirus (RSIV). Four oligonucleotide primer sets based on the ATPase gene, DNA polymerase gene, and a Pst I-restriction fragment of RSIV genomic DNA were synthesized. PCR products of the expected size were amplified with each primer set after 30 cycles using template ...
Jun Kurita +3 more
openaire +2 more sources
ABSTRACT Inland recirculating aquaculture is a thriving food industry providing sustainable and locally sourced high‐quality protein. However, its expansion is accompanied by emerging challenges regarding the spread of pathogens and diseases. Detection and management of pathogens in aquaculture remain underdeveloped compared to other animal farming ...
Jessica Rieder +3 more
wiley +1 more source

