Results 101 to 110 of about 1,760,212 (203)

Hidden Markov Models for Gene Sequence Classification: Classifying the VSG genes in the Trypanosoma brucei Genome

, 2015
The article presents an application of Hidden Markov Models (HMMs) for pattern recognition on genome sequences. We apply HMM for identifying genes encoding the Variant Surface Glycoprotein (VSG) in the genomes of Trypanosoma brucei (T.
Alvarez-Valin, Fernando, Basterrech, Sebastián, Guerberoff, Gustavo, Mesa, Andrea   +3 more
core   +1 more source

Selection of Reference Genes for the Normalization of RT-qPCR Data in Gene Expression Studies in Insects: A Systematic Review

Frontiers in Physiology, 2018
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for quantifying expression levels of targeted genes during various biological processes in numerous areas of clinical and biological research.
Jing Lü, Chunxiao Yang, Youjun Zhang, Huipeng Pan   +3 more
doaj   +1 more source

Identification of Four Soybean Reference Genes for Gene Expression Normalization

The Plant Genome, 2008
Gene expression analysis requires the use of reference genes constitutively expressed independently of tissues or environmental conditions. Housekeeping genes (e.g., actin, tubulin, ribosomal, polyubiquitin, and elongation factor 1‐α) are commonly used as reference genes with the assumption that they are uniformly expressed.
M. Libault, S. Thibivilliers, D. D. Bilgin, O. Radwan, M. Benitez, S. J. Clough, G. Stacey   +6 more
openaire   +2 more sources

Allelic diversity at orthologous candidate genes for drought tolerance in cereal crops: Example of the ASR gene family [PDF]

, 2008
Drought is a major abiotic stress. Many candidate genes have been proposed during the last years to explain aspects of tolerance to drought stress, for a specific crop and in a specific environment.
Baum, Michaël, Billot, Claire, Courtois, Brigitte, D'Hont, Angélique, Hash, Charles Tom, McNally, Kenneth L., Philippe, Romain, This, Dominique, Zivy, Michel   +8 more
core  

Candidate Reference Genes Selection and Application for RT-qPCR Analysis in Kenaf with Cytoplasmic Male Sterility Background

Frontiers in Plant Science, 2017
Cytoplasmic male sterility (CMS) is a maternally inherited trait that results in the production of dysfunctional pollen. Based on reliable reference gene-normalized real-time quantitative PCR (RT-qPCR) data, examining gene expression profile can provide ...
Bujin Zhou, Peng Chen, Aziz Khan, Yanhong Zhao, Lihong Chen, Dongmei Liu, Xiaofang Liao, Xiangjun Kong, Ruiyang Zhou   +8 more
doaj   +1 more source

Comparative Evaluation of Computational Methods for Validating Housekeeping Gene RT-qPCR Data in 3T3-L1 Cells

Biomedicines
Background: Postbiotics with anti-adipogenic properties can significantly modify adipocyte metabolism by influencing key cellular pathways involved in lipid accumulation.
Zhenya Ivanova, Natalia Grigorova, Valeria Petrova, Ekaterina Vachkova, Georgi Beev   +4 more
doaj   +1 more source

Reference genes in aging: what are you referring to?

Aging, 2023
Mishra, Manish, Howlett, Susan E.
openaire   +2 more sources

Evolution of gene families involved in banana fruit development and ripening : W077 [PDF]

, 2013
A reference genome sequence of banana was recently obtained from a Musa acuminata doubled-haploid accession (DH-Pahang, 523 Mb) and organized into eleven pseudomolecules. This genome opens brand new perspectives for the identification of genes underlying
Cardi, Céline, D'Hont, Angélique, Dasilva, C., Jourda, Cyril, Labadie, Karine, Mbéguié-A-Mbéguié, Didier, Yahiaoui, Nabila   +6 more
core  

Evaluation of suitable reference genes for normalization of quantitative reverse transcription PCR analyses in Clavibacter michiganensis

MicrobiologyOpen, 2019
Clavibacter michiganensis, the causal agent of bacterial canker of tomato, is a Gram‐positive bacterium and a model for studying plant diseases. The real‐time quantitative reverse transcription PCR (real‐time qRT‐PCR) assay is widely used to quantify ...
Na Jiang, Qingyang Lyu, Sining Han, Xin Xu, Ronald R. Walcott, Jianqiang Li, Laixin Luo   +6 more
doaj   +1 more source

Identification and Validation of qRT-PCR Reference Genes for Analyzing Arabidopsis Responses to High-Temperature Stress

Current Issues in Molecular Biology
Quantitative real-time PCR (qRT-PCR) is an essential tool for analyzing and selecting stable reference genes. In order to screen for suitable reference genes under high-temperature stress conditions in Arabidopsis, this study measured the relative ...
Siyu Chen, Qi Cai, Peipei Liu, Jingru Liu, Guanzhou Chen, Hanwei Yan, Han Zheng   +6 more
doaj   +1 more source