Results 111 to 120 of about 1,765,900 (302)

Housekeeping genes for quantitative expression studies in the three-spined stickleback Gasterosteus aculeatus [PDF]

, 2008
Background During the last years the quantification of immune response under immunological challenges, e.g. parasitation, has been a major focus of research.
A Bas, A Radonic, A Szabo, A Zapata, AL Filby, B Liss, C Ramakers, CL Andersen, EO Koppang, F Haller, G Rauch, HC Ingerslev, J Vandesompele, Joern P Scharsack, JP Scharsack, K Pechhold, KM Wegner, MF Oleksiak, MW Pfaffl, N Nicot, PA Olsvik, S Becker, SA Bustin, SA Bustin, Sascha Hibbeler, SF Gonzalez, SM Jorgensen, Sven Becker, TBH Reusch, V Ransbotyn   +29 more
core   +3 more sources

Molecular bases of circadian magnesium rhythms across eukaryotes

FEBS Letters, EarlyView.
Circadian rhythms in intracellular [Mg2+] exist across eukaryotic kingdoms. Central roles for Mg2+ in metabolism suggest that Mg2+ rhythms could regulate daily cellular energy and metabolism. In this Perspective paper, we propose that ancestral prokaryotic transport proteins could be responsible for mediating Mg2+ rhythms and posit a feedback model ...
Helen K. Feord, Gerben van Ooijen
wiley   +1 more source

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

FEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang, Satoshi Hashimoto, Sota Ito, Chisato Kikuguchi, Miho Hoshi, Kiyoshi Yamaguchi, Yoichi Furukawa, Toru Suzuki, Toshifumi Inada   +8 more
wiley   +1 more source

Selection and Validation of Reference Genes in Dendrocalamus brandisii for Quantitative Real-Time PCR

Plants
Dendrocalamus brandisii (Munro) Kurz is a sympodial bamboo species with a wide distribution in tropical and subtropical regions. Due to its remarkable regenerative ability and exceptional flavor, this species plays a pivotal role in bolstering the ...
Jutang Jiang, Changhong Mu, Yucong Bai, Wenlong Cheng, Ruiman Geng, Junlei Xu, Yuping Dou, Zhanchao Cheng, Jian Gao   +8 more
doaj   +1 more source

Evaluation of reference genes for gene expression studies in pig muscle tissue by real-time PCR

Czech Journal of Animal Science, 2011
The selection of reference genes is essential for gene expression studies when using a real-time quantitative polymerase chain reaction (PCR). Reference gene selection should be performed for each experiment because the gene expression level may be ...
M. Nesvadbová, A. Knoll
doaj   +1 more source

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

Selection of Reference Genes in Siraitia siamensis and Expression Patterns of Genes Involved in Mogrosides Biosynthesis

Plants
Siraitia siamensis is a traditional Chinese medicinal herb. In this study, using S. siamensis cultivated in vitro, twelve candidate reference genes under various treatments were analyzed for their expression stability by using algorithms such as GeNorm ...
Wenqiang Chen, Xiaodong Lin, Yan Wang, Detian Mu, Changming Mo, Huaxue Huang, Huan Zhao, Zuliang Luo, Dai Liu, Iain W. Wilson, Deyou Qiu, Qi Tang   +11 more
doaj   +1 more source

Reference gene screening of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans for quantitative real-time PCR studies [PDF]

, 2019
Real-time quantitative PCR studies largely depend on reference genes for the normalization of gene expression. Stable reference genes should be accurately selected in order to obtain reliable results.
Martel, An, Pasmans, Frank, Verbrugghe, Elin   +2 more
core   +1 more source

Reference Standards for Gene and Cell Therapy Products [PDF]

Molecular Therapy, 2017
Reference standards are important tools for the calibration of medical products and procedures. They are particularly important when dosing and potency are critical parameters for patient safety and product efficacy.1 Reference standards benefit the field by fostering best practices for the manufacturing and testing of safe and efficacious medicinal ...
openaire   +2 more sources

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source