Reference Gene Validation for Quantitative PCR Analysis in 2D and 3D AML12 Hepatocyte Models
BiomedicinesBackground/Objectives: Advanced 3D cell culture techniques enhance the physiological relevance of in vitro models, while supporting the 3Rs principles (Reduction, Refinement, and Replacement) of animal experimentation.
Zhenya Ivanova +4 more
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Reference Genes Selection and Normalization of Oxidative Stress Responsive Genes upon Different Temperature Stress Conditions in Hypericum perforatum L [PDF]
, 2017 Reverse transcription-quantitative real-time PCR (RT-qPCR) is a widely used technique for gene expression analysis. The reliability of this method depends largely on the suitable selection of stable reference genes for accurate data normalization ...
Arnholdt-Schmitt, Birgit +3 more
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A framework for human microbiome research. [PDF]
, 2012 A variety of microbial communities and their genes (the microbiome) exist throughout the human body, with fundamental roles in human health and disease. The National Institutes of Health (NIH)-funded Human Microbiome Project Consortium has established a ...
Human Microbiome Project Consortium
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Functional characterization of two plant type I MADS-box genes in Arabidopsis thaliana : AGL40 and AGL62 : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Plant Biology at Massey University, Palmerston North, New Zealand [PDF]
, 2008 MADS-box transcription factors (TF) are a family of evolutionary conserved genes found across various eukaryotic species. Characterized by the conserved DNA binding MADS-box domain.
Kaji, Ryohei
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Journal of Extracellular BiologyUrinary extracellular vesicles (uEVs) are rich in valuable biomolecule information which are increasingly recognized as potential biomarkers for various diseases.
Xiao‐Xiao Zhu +11 more
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Selection of housekeeping genes for gene expression studies in larvae from flatfish using real-time PCR [PDF]
, 2008 Flatfish metamorphosis involves major physiological and morphological changes. Due to its importance in aquaculture and as a model for developmental studies, some gene expression studies have focused on the understanding of this process using ...
Infante, C.
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Identification of the expressome by machine learning on omics data. [PDF]
, 2019 Accurate annotation of plant genomes remains complex due to the presence of many pseudogenes arising from whole-genome duplication-generated redundancy or the capture and movement of gene fragments by transposable elements.
Briggs, Steven P +3 more
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InsectsParasitoid wasps play a crucial role in the efficient control of pests, a substantial menace to human health and well-being. Tetrastichus hagenowii (Ratzeburg) stands out as the most effective egg parasitoid wasp for controlling American cockroaches, but
Renke Dong +8 more
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Chimeric Gene Construction without Reference to Restriction Sites [PDF]
BioTechniques, 2002 Toshiro Shigaki, Kendal D. Hirschi
openaire +3 more sources