Results 141 to 150 of about 6,662,733 (309)
MicrobiologyOpen, 2019 Clavibacter michiganensis, the causal agent of bacterial canker of tomato, is a Gram‐positive bacterium and a model for studying plant diseases. The real‐time quantitative reverse transcription PCR (real‐time qRT‐PCR) assay is widely used to quantify ...
Na Jiang +6 more
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Outstanding Business Reference Sources: The 2006 Selection of Recent Titles
, 2007 A selection of business reference sources for ...
BRASS Business Reference Sources Committee
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Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
FEBS Letters, EarlyView.Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
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Current Issues in Molecular BiologyQuantitative real-time PCR (qRT-PCR) is an essential tool for analyzing and selecting stable reference genes. In order to screen for suitable reference genes under high-temperature stress conditions in Arabidopsis, this study measured the relative ...
Siyu Chen +6 more
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Outstanding Business Reference Sources: The 2004 Selection of Recent Titles
, 2005 A selection of business reference sources for ...
BRASS Business Reference Sources Committee
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FEBS Letters, EarlyView.Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante +10 more
wiley +1 more source
Reference Gene Validation for Quantitative PCR Analysis in 2D and 3D AML12 Hepatocyte Models
BiomedicinesBackground/Objectives: Advanced 3D cell culture techniques enhance the physiological relevance of in vitro models, while supporting the 3Rs principles (Reduction, Refinement, and Replacement) of animal experimentation.
Zhenya Ivanova +4 more
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Outstanding Business Reference Sources: The 2010 Selection of Recent Titles
, 2011 A selection of business reference sources for ...
BRASS Business Reference Sources Committee
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Evaluation of reference genes of
, 2014 Quantitative real-time polymerase chain reaction (qPCR) is probably the most used method for gene expression quantification because of its high sensitivity and specificity.
Beatriz Novoa +9 more
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Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry
FEBS Letters, EarlyView.EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri +5 more
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