Results 221 to 230 of about 6,952,897 (310)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Selection of reference genes and proteins for expression studies in pediatric gliomas. [PDF]

Discov Oncol
Nava-Salazar S, Pérez-González OA, Baranda-Ávila N, Marhx-Bracho A, Díaz-Ávalos C, Granados-Rojas L, Phillips-Farfán BV, Perezpeña-Diazconti M, Gutiérrez-Ospina G, Vanoye-Carlo A.   +9 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

The longitudinal expression of <i>P. aeruginosa</i> reference genes in infection-mimicking media. [PDF]

Microbiology (Reading)
Hibbert TM, Leighton HJ, Pottenger S, Neill DR, Fothergill JL.   +4 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Systematic Screening of Reference Genes for qPCR in <i>Procambarus clarkii</i>: <i>RPL13a</i> Shows Stable Expression Under Non-Ionic Ammonia Stress and Multiple Experimental Conditions. [PDF]

Biology (Basel)
Yuan X, Tian X, Liu Y, He Z, Li J, Fan Y, Guo J, He Y, Deng S, Wang D.   +9 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Validation of Stable Reference Genes for RT-qPCR Normalization in Oxycetonia jucunda (Coleoptera: Scarabaeidae). [PDF]

Insects
Zhao SH, Yue Y, Yu RT, Gao Q, Zhao JQ, Zhang SP, Zhou N, Xu GL.   +7 more
europepmc   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Identification and Validation of Reference Genes for Reliable RT-qPCR Normalization in <i>Schisandra chinensis</i> Across Different Tissues and Abiotic Stress Conditions. [PDF]

Plants (Basel)
Liang L, Song X, Zhang X, Liu Y, Shi G, Wang Z, Zhang C, Li C, Zhang X, Sun D, Ai J.   +10 more
europepmc   +1 more source