Results 271 to 280 of about 1,765,900 (302)
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A reference gene set for chemosensory receptor genes of Manduca sexta
Insect Biochemistry and Molecular Biology, 2015The order of Lepidoptera has historically been crucial for chemosensory research, with many important advances coming from the analysis of species like Bombyx mori or the tobacco hornworm, Manduca sexta. Specifically M. sexta has long been a major model species in the field, especially regarding the importance of olfaction in an ecological context ...
Koenig, C. +9 more
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Validation of reference genes in the feline endometrium
Reproductive Biology, 2014The aim of the study was to find the most stable reference genes from: ACTB, GAPDH, RPL30, CYC, RPL17, RPS7 and YWHAZ in the feline endometrium. Three free software packages, geNorm, NormFinder and BestKeeper were used. In geNorm analysis, the most stable gene was RPS7 (at a primer concentration 1000 nM) or YWHAZ (500 and 250 nM).
Ewelina, Jursza +2 more
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Reference standard for gene therapy closer
Molecular Medicine Today, 2000On both sides of the Atlantic, new initiatives to establish gene therapy benchmarks could stimulate research into rare conditions and allow researchers to compare their results with unprecedented accuracy.Certainly, there is a need to standardize gene therapy vectors.
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Light Intensity-Dependent Dysregulation of Retinal Reference Genes
2019The degeneration of photoreceptors is a common hallmark of ocular diseases like retinitis pigmentosa (RP) or age-related macular degeneration (AMD). To experimentally induce photoreceptor degeneration, the light damage paradigm is frequently used. In this study we show that the exposure to high amounts of cool white light (10,000 lux, 1 h) resulted in ...
Christina B, Bielmeier +2 more
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Evaluating reference genes to normalize gene expression in human epileptogenic brain tissues
Biochemical and Biophysical Research Communications, 2010Several reference genes have been used to quantify gene expression in human epilepsy surgery tissue. However, their reliability has not been validated in detail, although this is crucial in interpreting epilepsy-related changes of gene expression. We evaluated 12 potential reference genes in neocortical tissues resected from patients with temporal lobe
Stephan, Wierschke +6 more
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Reference genes for accurate normalization of gene expression in wood-decomposing fungi
Fungal Genetics and Biology, 2019Wood-decomposing fungi efficiently decompose plant lignocellulose, and there is increasing interest in characterizing and perhaps harnessing the fungal gene regulation strategies that enable wood decomposition. Proper interpretation of these fungal mechanisms relies on accurate quantification of gene expression, demanding reliable internal control ...
Jiwei, Zhang +5 more
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Reference-Based Gene Expression Analysis Using Galaxy Server
RNA sequencing (RNA-Seq) has become the gold standard for transcriptome analysis, making gene expression quantification the core application in molecular biology research. For well-annotated genomes, the standard RNA-Seq workflow relies on a reference genome-based approach.Sara, González-Bodí +1 more
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New reference genes in Trichoderma reesei
Trichoderma reesei is a highly significant producer of various enzymes used in industry and agriculture. Due to its relatively high secretion efficiency, it holds potential for a wide range of applications. However, the complexity of its gene expression mechanisms and the unpredictable behavior of newly developed genetically modified strains have made ...openaire +1 more source
A reference map of the human binary protein interactome
Nature, 2020Katja Luck, Dae-Kyum Kim, Luke Lambourne
exaly