Screening and Stability Validation of RT-qPCR Reference Genes in <i>Portulaca oleracea</i> L. in Diverse Tissues and Under Abiotic Stress Conditions. [PDF]
Int J Mol SciFang J +6 more
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Evaluation of candidate RT-qPCR reference genes in the aging African turquoise killifish brain. [PDF]
Aging BrainWhisenant E, Lekven AC.
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Evaluation of reference genes for accurate qRT-PCR normalization in bitter gourd across different tissues and stress conditions. [PDF]
BMC Plant BiolZeng S +9 more
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Reference Genes in Real-Time PCR [PDF]
Journal of Applied Genetics, 2013 This paper aims to discuss various aspects of the use of reference genes in qPCR technique used in the thousands of present studies. Most frequently, these are housekeeping genes and they must meet several criteria so that they can lay claim to the name.
Marcin Rapacz
exaly +3 more sources
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Selection of reference genes for gene expression studies in astrocytomas
Analytical Biochemistry, 2011This study was aimed to test a panel of six housekeeping genes (GAPDH, HPRT1, POLR2A, RPLP0, ACTB, and H3F) so as to identify and validate the most suitable reference genes for expression studies in astrocytomas. GAPDH was the most stable and HPRT1 was the least stable reference gene.
Sylwia M, Gresner +5 more
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Identification of Stable Reference Genes for Toxicogenomic and Gene Expression Analysis
2021Gene expression analysis has been becoming a popular method for studying gene function and response to different environmental stresses, including toxin/pollution exposure. Selection of a suitable reference gene is critically important for gene expression analysis due to that wrong reference genes will cause misleading and even wrong conclusion. A good
Xiaoping, Pan, Baohong, Zhang
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Validation of reference genes in the feline endometrium
Reproductive Biology, 2014The aim of the study was to find the most stable reference genes from: ACTB, GAPDH, RPL30, CYC, RPL17, RPS7 and YWHAZ in the feline endometrium. Three free software packages, geNorm, NormFinder and BestKeeper were used. In geNorm analysis, the most stable gene was RPS7 (at a primer concentration 1000 nM) or YWHAZ (500 and 250 nM).
Ewelina, Jursza +2 more
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Candidate reference genes for gene expression studies in water lily
Analytical Biochemistry, 2010The selection of an appropriate reference gene(s) is a prerequisite for the proper interpretation of quantitative Real-Time polymerase chain reaction data. We report the evaluation of eight candidate reference genes across various tissues and treatments in the water lily by the two software packages geNorm and NormFinder.
Huolin, Luo +5 more
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A reference gene set for chemosensory receptor genes of Manduca sexta
Insect Biochemistry and Molecular Biology, 2015The order of Lepidoptera has historically been crucial for chemosensory research, with many important advances coming from the analysis of species like Bombyx mori or the tobacco hornworm, Manduca sexta. Specifically M. sexta has long been a major model species in the field, especially regarding the importance of olfaction in an ecological context ...
Koenig, C. +9 more
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Rice Reference Genes: redefining reference genes in rice by mining RNA-seq datasets
Plant and Cell PhysiologyAbstract Reverse transcription quantitative real-time PCR (RT-qPCR) is esteemed for its precision and reliability, positioning it as the standard for evaluating gene expression. Selecting suitable reference genes is crucial for acquiring accurate data on target gene expression.
Xin Liu +9 more
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