Results 271 to 280 of about 6,662,733 (309)

Selection and evaluation of reference genes for qRT-PCR in Inonotus obliquus. [PDF]

Front Microbiol
Li L, Guo X, Wang S.
europepmc   +1 more source

Selection and validation of reference genes for RT-qPCR normalization in dormant cancer cells. [PDF]

Sci Rep
Iskhakova ER, Aleksandrova KV, Suvorova II.   +2 more
europepmc   +1 more source

Selection and evaluation of reference genes for quantitative real-time polymerase chain reaction normalization in Pieris melete (Lepidoptera, Pieridae). [PDF]

J Insect Sci
Jiang T, Liu X, Zou N, Zhang W, Peng Y, Xiao H.   +5 more
europepmc   +1 more source

Identification of stable reference genes in Edwardsiella ictaluri for accurate gene expression analysis. [PDF]

PLoS One
Lu J, Abdelhamed H, Al-Janabi N, Eissa N, Lawrence M, Karsi A.   +5 more
europepmc   +1 more source

Reference genes for measuring mRNA expression [PDF]

Theory in Biosciences, 2012
The aim of this review is to find answers to some of the questions surrounding reference genes and their reliability for quantitative experiments. Reference genes are assumed to be at a constant expression level, over a range of conditions such as temperature.
Maurice Ling
exaly   +7 more sources

Reference genes in real-time PCR [PDF]

Journal of Applied Genetics, 2013
This paper aims to discuss various aspects of the use of reference genes in qPCR technique used in the thousands of present studies. Most frequently, these are housekeeping genes and they must meet several criteria so that they can lay claim to the name.
Marcin Rapacz
exaly   +3 more sources

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Selection of reference genes for gene expression studies in astrocytomas

Analytical Biochemistry, 2011
This study was aimed to test a panel of six housekeeping genes (GAPDH, HPRT1, POLR2A, RPLP0, ACTB, and H3F) so as to identify and validate the most suitable reference genes for expression studies in astrocytomas. GAPDH was the most stable and HPRT1 was the least stable reference gene.
Sylwia M, Gresner, Ewa, Golanska, Dominika, Kulczycka-Wojdala, Dariusz J, Jaskolski, Wielislaw, Papierz, Pawel P, Liberski   +5 more
openaire   +2 more sources

Identification of Stable Reference Genes for Toxicogenomic and Gene Expression Analysis

2021
Gene expression analysis has been becoming a popular method for studying gene function and response to different environmental stresses, including toxin/pollution exposure. Selection of a suitable reference gene is critically important for gene expression analysis due to that wrong reference genes will cause misleading and even wrong conclusion. A good
Xiaoping, Pan, Baohong, Zhang
openaire   +2 more sources

Validation of reference genes in the feline endometrium

Reproductive Biology, 2014
The aim of the study was to find the most stable reference genes from: ACTB, GAPDH, RPL30, CYC, RPL17, RPS7 and YWHAZ in the feline endometrium. Three free software packages, geNorm, NormFinder and BestKeeper were used. In geNorm analysis, the most stable gene was RPS7 (at a primer concentration 1000 nM) or YWHAZ (500 and 250 nM).
Ewelina, Jursza, Dariusz J, Skarzynski, Marta J, Siemieniuch   +2 more
openaire   +2 more sources

Candidate reference genes for gene expression studies in water lily

Analytical Biochemistry, 2010
The selection of an appropriate reference gene(s) is a prerequisite for the proper interpretation of quantitative Real-Time polymerase chain reaction data. We report the evaluation of eight candidate reference genes across various tissues and treatments in the water lily by the two software packages geNorm and NormFinder.
Huolin, Luo, Sumei, Chen, Hongjian, Wan, Fadi, Chen, Chunsun, Gu, Zhaolei, Liu   +5 more
openaire   +2 more sources