Results 71 to 80 of about 1,760,212 (203)

Evaluation of reference genes for insect olfaction studies [PDF]

open access: yesParasites & Vectors, 2015
La PCR cuantitativa de transcripción inversa (qRT-PCR) es un método robusto y accesible para analizar la expresión génica e inferir la regulación génica. Al ser una cadena de procedimientos, esta técnica está sujeta a errores sistemáticos debido a limitaciones biológicas y técnicas establecidas principalmente por el material de partida y los ...
Bonaventure Aman Omondi   +4 more
openaire   +4 more sources

Selection of Reference Genes in Evodia rutaecarpa var. officinalis and Expression Patterns of Genes Involved in Its Limonin Biosynthesis

open access: yesPlants, 2023
E. rutaecarpa var. officinalis is a traditional Chinese medicinal plant known for its therapeutic effects, which encompass the promotion of digestion, the dispelling of cold, the alleviation of pain, and the exhibition of anti-inflammatory and ...
Yu Zhou   +10 more
doaj   +1 more source

How to perform RT-qPCR accurately in plant species?: a case study on flower colour gene expression in an azalea (Rhododendron simsii hybrids) mapping population [PDF]

open access: yes, 2013
Background: Flower colour variation is one of the most crucial selection criteria in the breeding of a flowering pot plant, as is also the case for azalea (Rhododendron simsii hybrids).
De Keyser, Ellen   +3 more
core   +2 more sources

Reference genes for livestock gene expression profiling – Literature review

open access: yesActa Agraria Debreceniensis, 2017
Quantitative real-time polymerase chain reaction (qPCR) is an essential tool for understanding animal cell’s response to developmental progression or to different experimental conditions at gene expression level. However the reliability of this method heavily lies on proper normalization (measuring a target and a reference gene’s expression from the ...
Ádám Simon   +3 more
openaire   +2 more sources

Evaluation of Reference Genes for Quantitative Real-Time PCR Analysis in the Bean Bug, Riptortus pedestris (Hemiptera: Alydidae)

open access: yesInsects, 2023
Quantitative real-time PCR (qRT-PCR) is widely accepted as a precise and convenient method for quantitatively analyzing the expression of functional genes. The data normalization strongly depends upon stable reference genes.
Liuyang Wang   +6 more
doaj   +1 more source

AutDB: a gene reference resource for autism research [PDF]

open access: yesNucleic Acids Research, 2008
Recent advances in studies of Autism Spectrum Disorders (ASD) has uncovered many new candidate genes and continues to do so at an accelerated pace. To address the genetic complexity of ASD, we have developed AutDB (http://www.mindspec.org/autdb.html), a publicly available web-portal for on-going collection, manual annotation and visualization of genes ...
Basu, Saumyendra N.   +2 more
openaire   +2 more sources

Evaluation of eleven reference genes for Reverse Transcriptase Quantitative PCR of rubber tree under water deficit [PDF]

open access: yes, 2015
Reverse Transcriptase Quantitative PCR (RTqPCR) is a powerful technique in order to detect low abundance of mRNA in the plant cell. The measurement of transcript abundance is relative to the control of expression such as housekeeping genes.
Leclercq, Julie   +2 more
core  

Postmenopausal Osteoporosis reference genes for qPCR expression assays [PDF]

open access: yesScientific Reports, 2019
AbstractOsteoporosis (OP) is a multifactorial disease influenced by genetic factors in more than half of the cases. In spite of the efforts to clarify the relationship among genetic factors and susceptibility to develop OP, many genetic associations need to be further functionally validated.
Camilla Albertina Dantas de Lima   +6 more
openaire   +4 more sources

Area Postrema [PDF]

open access: yes, 2008
This report contains a gene expression summary of the area postrema (AP), derived from the "Allen Brain Atlas":http://www.brain-map.org/welcome.do;jsessionid=EDE40ADC940845D169DE378ADC9B71BD (ABA) in-situ hybridization (ISH) mouse data set ...
Allen Institute for Brain Science   +3 more
core   +2 more sources

De novo assembly of the cattle reference genome with single-molecule sequencing. [PDF]

open access: yes, 2020
BackgroundMajor advances in selection progress for cattle have been made following the introduction of genomic tools over the past 10-12 years. These tools depend upon the Bos taurus reference genome (UMD3.1.1), which was created using now-outdated ...
Bickhart, Derek M   +32 more
core  

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