Results 71 to 80 of about 6,662,733 (309)

Reliable reference genes and abiotic stress marker genes in Klebsormidium nitens

Scientific Reports, 2022
AbstractMicroalgae have recently emerged as a key research topic, especially as biological models. Among them, the green alga Klebsormidium nitens, thanks to its particular adaptation to environmental stresses, represents an interesting photosynthetic eukaryote for studying the transition stages leading to the colonization of terrestrial life.
Chatelain, Pauline, Blanchard, Cécile, Astier, Jeremy, Klinguer, Agnès, Wendehenne, David, Jeandroz, Sylvain, Rosnoblet, Claire   +6 more
openaire   +5 more sources

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Identification and selection of reference genes for analysis of gene expression by quantitative real-time PCR in the euhalophyte Suaeda altissima (L.) Pall.

Communicative & Integrative Biology
Сoding sequences of seven housekeeping genes: actin SaACT7, ubiquitin-conjugating protein SaUBC10, glyceraldehyde-3-phosphate dehydrogenase SaGAPDH, protein of the large subunit of ribosomes SaL2, α-tubulin SaTUA, translation elongation factor SaeEF1α ...
Dmitrii E. Khramov, Olga I. Nedelyaeva, Alena O. Konoshenkova, Vadim S. Volkov, Yurii V. Balnokin   +4 more
doaj   +1 more source

Reference genes for quantitative real-time PCR analysis and quantitative expression of P5CS in Agropyron mongolicum under drought stress

Journal of Integrative Agriculture, 2016
Reference genes, stably expressing in different tissues and cells, are commonly used as the references in expression analysis. Selecting the optimum reference gene is crucial to the success of experiments.
Qing-song TIAN, Shu-yan WANG, Jian-cai DU, Zhi-juan WU, Xiao-quan LI, Bing HAN   +5 more
doaj   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Selection of Reference Genes by Quantitative Real-Time PCR in Different Cell Lines from Humpback Grouper (Cromileptes altivelis)

Fishes
Humpback grouper (Cromileptes altivelis) is an economically important fish, but the increasing density of its farming has led to more severe disease outbreaks.
Xiangyu Du, Han Zhang, Longfei Zhu, Zhenjie Cao, Chen Zhang, Ying Wu, Yongcan Zhou, Yun Sun   +7 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Embryo reference genes: additional validation

, 2021
This file contains extended data: pairwise comparison data for geNorm, and additional validation of the reference genes identified in the following manuscript:  Identification of qPCR reference genes suitable for normalising gene expression in the ...
Richard Piercy (10213257), John Hildyard (2556154), Dominic Wells (10213256)   +2 more
core   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Mitigation of the effect of variability in digital PCR assays through use of duplexed reference assays for normalization

BioTechniques, 2018
Digital PCR has been promoted as a technique for obtaining absolute measures of the amount of nucleic acid target sequence in a sample, but still lacks standardization in data reporting.
Sherry J Coulter
doaj   +1 more source