Results 1 to 10 of about 116,375 (241)

Rapid and Sensitive Detection of Repetitive Nucleic Acid Sequences Using Magnetically Modulated Biosensors [PDF]

open access: yesACS Omega, 2019
Repetitive DNA sequences are abundant in the genome of most biological species. These sequences are naturally "preamplified", which makes them a preferential target for a variety of biological assays. Current methods to detect specific DNA sequences are based on the quantitative polymerase chain reaction (PCR), which relies on target amplification by ...
Michael Margulis, Amos Danielli
doaj   +5 more sources

Advances in the application of CRISPR-Cas technology in rapid detection of pathogen nucleic acid

open access: yesFrontiers in Molecular Biosciences, 2023
Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) are widely used as gene editing tools in biology, microbiology, and other fields.
Xiaoping Li   +18 more
doaj   +2 more sources

Extracellular vesicle-associated repetitive element DNAs as candidate osteosarcoma biomarkers

open access: yesScientific Reports, 2021
Osteosarcoma (OS) is the most common malignant bone tumor in children and young adults. Despite that high-risk factors have been identified, no test for early detection is available.
Linda Cambier   +8 more
doaj   +3 more sources

Fast and non-toxic in situ hybridization without blocking of repetitive sequences. [PDF]

open access: yesPLoS ONE, 2012
Formamide is the preferred solvent to lower the melting point and annealing temperature of nucleic acid strands in in situ hybridization (ISH). A key benefit of formamide is better preservation of morphology due to a lower incubation temperature. However,
Steen H Matthiesen, Charles M Hansen
doaj   +2 more sources

Comprehensive Regulation of Liquid–Liquid Phase Separation of Polypeptides

open access: yesMolecules, 2023
The elucidation of the molecular driving forces responsible for Liquid–liquid Phase Separation (LLPS) of proteins and nucleic acids within living cells is crucial for understanding its biological functions and its role in related diseases. In the present
Yanwei Wang   +3 more
doaj   +2 more sources

Genomics-Guided Drawing of Molecular and Pathophysiological Components of Malignant Regulatory Signatures Reveals a Pivotal Role in Human Diseases of Stem Cell-Associated Retroviral Sequences and Functionally-Active hESC Enhancers

open access: yesFrontiers in Oncology, 2021
Repetitive DNA sequences (repeats) colonized two-third of human genome and a majority of repeats comprised of transposable genetic elements (TE). Evolutionary distinct categories of TE represent nucleic acid sequences that are repeatedly copied from and ...
Gennadi V. Glinsky, Gennadi V. Glinsky
doaj   +2 more sources

RNA-dependent stabilization of SUV39H1 at constitutive heterochromatin

open access: yeseLife, 2017
Heterochromatin formed by the SUV39 histone methyltransferases represses transcription from repetitive DNA sequences and ensures genomic stability. How SUV39 enzymes localize to their target genomic loci remains unclear.
Whitney L Johnson   +7 more
doaj   +2 more sources

Targeting glioma stem cells in vivo by a G-quadruplex-stabilizing synthetic macrocyclic hexaoxazole

open access: yesScientific Reports, 2017
G-quadruplex (G4) is a higher-order nucleic acid structure that is formed by guanine-rich sequences. G4 stabilization by small-molecule compounds called G4 ligands often causes cytotoxicity, although the potential medicinal impact of this effect has not ...
Takahiro Nakamura   +10 more
doaj   +2 more sources

Complete chloroplast genomes and phylogenetic relationships of Pedicularis chinensis and Pedicularis kansuensis

open access: yesScientific Reports
The complete cp genomes of Pedicularis chinensis (GenBank accession number: OQ587614) and Pedicularis kansuensis (GenBank accession number: OQ587613) were sequenced, assembled, and annotated. Their chloroplast (cp) genome lengths were 146,452 bp, and 146,
Tao Wang   +7 more
doaj   +2 more sources

The Simple Biology of Flipons and Condensates Enhances the Evolution of Complexity

open access: yesMolecules, 2021
The classical genetic code maps nucleotide triplets to amino acids. The associated sequence composition is complex, representing many elaborations during evolution of form and function. Other genomic elements code for the expression and processing of RNA
Alan Herbert
doaj   +1 more source

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