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Rapid and Sensitive Detection of Repetitive Nucleic Acid Sequences Using Magnetically Modulated Biosensors [PDF]
Repetitive DNA sequences are abundant in the genome of most biological species. These sequences are naturally "preamplified", which makes them a preferential target for a variety of biological assays. Current methods to detect specific DNA sequences are based on the quantitative polymerase chain reaction (PCR), which relies on target amplification by ...
Michael Margulis, Amos Danielli
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Advances in the application of CRISPR-Cas technology in rapid detection of pathogen nucleic acid
Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) are widely used as gene editing tools in biology, microbiology, and other fields.
Xiaoping Li+18 more
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Extracellular vesicle-associated repetitive element DNAs as candidate osteosarcoma biomarkers
Osteosarcoma (OS) is the most common malignant bone tumor in children and young adults. Despite that high-risk factors have been identified, no test for early detection is available.
Linda Cambier+8 more
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Fast and non-toxic in situ hybridization without blocking of repetitive sequences. [PDF]
Formamide is the preferred solvent to lower the melting point and annealing temperature of nucleic acid strands in in situ hybridization (ISH). A key benefit of formamide is better preservation of morphology due to a lower incubation temperature. However,
Steen H Matthiesen, Charles M Hansen
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Comprehensive Regulation of Liquid–Liquid Phase Separation of Polypeptides
The elucidation of the molecular driving forces responsible for Liquid–liquid Phase Separation (LLPS) of proteins and nucleic acids within living cells is crucial for understanding its biological functions and its role in related diseases. In the present
Yanwei Wang+3 more
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Repetitive DNA sequences (repeats) colonized two-third of human genome and a majority of repeats comprised of transposable genetic elements (TE). Evolutionary distinct categories of TE represent nucleic acid sequences that are repeatedly copied from and ...
Gennadi V. Glinsky, Gennadi V. Glinsky
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RNA-dependent stabilization of SUV39H1 at constitutive heterochromatin
Heterochromatin formed by the SUV39 histone methyltransferases represses transcription from repetitive DNA sequences and ensures genomic stability. How SUV39 enzymes localize to their target genomic loci remains unclear.
Whitney L Johnson+7 more
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Targeting glioma stem cells in vivo by a G-quadruplex-stabilizing synthetic macrocyclic hexaoxazole
G-quadruplex (G4) is a higher-order nucleic acid structure that is formed by guanine-rich sequences. G4 stabilization by small-molecule compounds called G4 ligands often causes cytotoxicity, although the potential medicinal impact of this effect has not ...
Takahiro Nakamura+10 more
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The complete cp genomes of Pedicularis chinensis (GenBank accession number: OQ587614) and Pedicularis kansuensis (GenBank accession number: OQ587613) were sequenced, assembled, and annotated. Their chloroplast (cp) genome lengths were 146,452 bp, and 146,
Tao Wang+7 more
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The Simple Biology of Flipons and Condensates Enhances the Evolution of Complexity
The classical genetic code maps nucleotide triplets to amino acids. The associated sequence composition is complex, representing many elaborations during evolution of form and function. Other genomic elements code for the expression and processing of RNA
Alan Herbert
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