Results 21 to 30 of about 419 (189)

Systematic identification of stem-loop containing sequence families in bacterial genomes

BMC Genomics, 2008
Background Analysis of non-coding sequences in several bacterial genomes brought to the identification of families of repeated sequences, able to fold as secondary structures.
Silvestro Giustina, Petrillo Mauro, Cozzuto Luca, Di Nocera Pier, Paolella Giovanni   +4 more
doaj   +1 more source

Semipermeable Cellulose Beads Allow Selective and Continuous Release of Small Extracellular Vesicles (sEV) From Encapsulated Cells

Frontiers in Pharmacology, 2020
The clinical benefit of therapies using Mesenchymal Stem Cells (MSCs) is attributable to their pleiotropic effect over cells and tissues, mainly through their secretome.
Gabriela Zavala, Gabriela Zavala, María-Paz Ramos, María-Paz Ramos, Aliosha I. Figueroa-Valdés, Aliosha I. Figueroa-Valdés, Pablo Cisternas, Ursula Wyneken, Macarena Hernández, Macarena Hernández, Pauline Toa, Brian Salmons, John Dangerfield, Walter H. Gunzburg, Maroun Khoury, Maroun Khoury, Maroun Khoury   +16 more
doaj   +1 more source

Regulation and function of R-loops at repetitive elements [PDF]

, 2023
R-loops are atypical, three-stranded nucleic acid structures that contain a stretch of RNA:DNA hybrids and an unpaired, single stranded DNA loop. R-loops are physiological relevant and can act as regulators of gene expression, chromatin structure, DNA ...
Boncristiani, Chiara, Alice Gambelli, Ferrando, Alessandro, Gambelli, Alice, Alessandro Ferrando, Chiara Boncristiani, Stefan Schoeftner, Schoeftner, Stefan   +7 more
core   +1 more source

RPiRLS: Quantitative Predictions of RNA Interacting with Any Protein of Known Sequence

Molecules, 2018
RNA-protein interactions (RPIs) have critical roles in numerous fundamental biological processes, such as post-transcriptional gene regulation, viral assembly, cellular defence and protein synthesis.
Wen-Jun Shen, Wenjuan Cui, Danze Chen, Jieming Zhang, Jianzhen Xu   +4 more
doaj   +1 more source

Targeting glioma stem cells in vivo by a G-quadruplex-stabilizing synthetic macrocyclic hexaoxazole

Scientific Reports, 2017
G-quadruplex (G4) is a higher-order nucleic acid structure that is formed by guanine-rich sequences. G4 stabilization by small-molecule compounds called G4 ligands often causes cytotoxicity, although the potential medicinal impact of this effect has not ...
Takahiro Nakamura, Sachiko Okabe, Haruka Yoshida, Keisuke Iida, Yue Ma, Shogo Sasaki, Takao Yamori, Kazuo Shin-ya, Ichiro Nakano, Kazuo Nagasawa, Hiroyuki Seimiya   +10 more
doaj   +1 more source

Optimized Lysis-Extraction Method Combined With IS6110-Amplification for Detection of Mycobacterium tuberculosis in Paucibacillary Sputum Specimens

Frontiers in Microbiology, 2018
Background: When available, nucleic acid tests (NATs) offer powerful tools to strengthen the potential of tuberculosis (TB) diagnosis assays. The sensitivity of molecular assays is critical for detection of Mycobacterium tuberculosis (MTB) in ...
Pratt Kolia-Diafouka, Sylvain Godreuil, Arnaud Bourdin, Severine Carrère-Kremer, Laurent Kremer, Philippe Van de Perre, Edouard Tuaillon   +6 more
doaj   +1 more source

Ubiquitination of secretory granules promotes their crinophagic degradation in Drosophila

FEBS Letters, EarlyView.
Ubiquitination of secretory granules in Drosophila larval salivary glands is a critical molecular trigger for crinophagy, the lysosomal degradation of unreleased, or low‐quality granules. The E3 ubiquitin ligase Cnot4 is recruited to the surface of secretory granules to induce crinophagy.
Tamás Csizmadia, Anna Dósa, Asha Kiran Maddali, András Jipa, Hajnalka Laczkó‐Dobos, Péter Lőw, Gábor Juhász   +6 more
wiley   +1 more source

Molecular genetic studies on Brassica napus L. [PDF]

, 1991
The feasibility of using two different methods of assaying for DNA polymer phisms has been assessed. They were Restriction fragment length polymorphisms (RFLPs) as revealed by a range of characterised Brassica cDNA sequences and Random amplified ...
Napis, Suhaimi, Napis, S
core  

Detection of chromosome aberrations in the human interphase nucleus by visualization of specific target DNAs with radioactive and non-radioactive in situ hybridization techniques: diagnosis of trisomy 18 with probe L1.84 [PDF]

, 1986
The localization of chromosome 18 in human interphase nuclei is demonstrated by use of radioactive and nonradioactive in situ hybridization techniques with a DNA clone designated L1.84.
Pearson, P., P. Devilee, Schardin, Margit, M. Schardin, A. Brückner, Brückner, A., Cremer, Thomas, M. van der Ploeg, Landegent, J., T. Cremer, J. Landegent, Devilee, P., P. Pearson, Scholl, Hans Peter, Van Der Ploeg, Mels, Hager, H. D., H. P. Scholl, H. D. Hager   +17 more
core   +1 more source

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source