Results 181 to 190 of about 1,350,402 (318)
ATP is dispensable for E. coli DNA replication and eukaryotic helicase activity. [PDF]
Spinks RR +10 more
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Data from CREP Replication of Does Money Buy Happiness, Diener et al. (2010). [PDF]
Costa O +3 more
europepmc +1 more source
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
Topoisomerase III limits RecA-dependent DNA amplification in the chromosome terminus with RecG. [PDF]
Dadras A, Le Campion A, Drolet M.
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
The role of protein kinases in DNA replication in Saccharomyces cerevisiae
The initiation of DNA replication at the onset of S phase in eukaryotic cells is a critically important and tightly regulated process. Multiple origins of replication in the genome must be co-ordinately regulated such that duplication of the chromosomes ...
Sweet, S.
core
Processing of reversed replication forks is required for the resolution of replication-transcription conflicts. [PDF]
Carvajal-Garcia J, Merrikh H.
europepmc +1 more source
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao +3 more
wiley +1 more source
RepliSage: a stochastic graph-based framework for 3D chromatin modeling across the cell cycle. [PDF]
Korsak S +8 more
europepmc +1 more source

