Results 141 to 150 of about 230,046 (255)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

DNA replication initiation shapes the mutational landscape and expression of the human genome. [PDF]

Sci Adv, 2022
Murat P, Perez C, Crisp A, van Eijk P, Reed SH, Guilbaud G, Sale JE.   +6 more
europepmc   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Nuclear DAB2IP regulates DNA replication initiation through activating PLK1-mediated HBO1 phosphorylation. [PDF]

Nucleic Acids Res
Shang ZF, Yu L, Newman C, Chen WM, Birdsong GW, Sharp BC, Story MD, Saha D, Davis AJ.   +8 more
europepmc   +1 more source

Robust linear DNA degradation supports replication-initiation-defective mutants in Escherichia coli. [PDF]

G3 (Bethesda), 2022
Rao TVP, Kuzminov A.
europepmc   +1 more source

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

FEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart, Manon Van den Abbeel, Christoph Schifflers, Karim Bouhjar, Andrea Scarmelotto, Alexis Khelfi, Anne‐Catherine Wera, Carine Michiels   +7 more
wiley   +1 more source

Bioinformatics Analysis Reveals that Minichromosome Maintenance 10 Replication Initiation Factor Is a Possible Prognostic Marker for Patients With Adrenocortical Carcinoma. [PDF]

Cancer Diagn Progn
Yamashita SN, Tanaka Y, Islam S, Kitagawa T, Kazuhiro T, Paudel D, Giri S, Ohta T, Harada F, Nagayasu H, Kuramitsu Y.   +10 more
europepmc   +1 more source

Rep protein accommodates together dsDNA and ssDNA which enables a loop-back mechanism to plasmid DNA replication initiation. [PDF]

Nucleic Acids Res, 2023
Wegrzyn K, Oliwa M, Nowacka M, Zabrocka E, Bury K, Purzycki P, Czaplewska P, Pipka J, Giraldo R, Konieczny I.   +9 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Mutant p53 variants differentially impact replication initiation and activate cGAS-STING to affect immune checkpoint inhibition. [PDF]

Commun Biol
Liu K, Garan LAW, Lin FT, Lin WC.
europepmc   +1 more source