Results 1 to 10 of about 3,588,418 (239)

Mechanism of Origin DNA Recognition and Assembly of an Initiator-Helicase Complex by SV40 Large Tumor Antigen

Cell Reports, 2013
The DNA tumor virus Simian virus 40 (SV40) is a model system for studying eukaryotic replication. SV40 large tumor antigen (LTag) is the initiator/helicase that is essential for genome replication.
Y. Paul Chang, Meng Xu, Ana Carolina Dantas Machado, Xian Jessica Yu, Remo Rohs, Xiaojiang S. Chen   +5 more
doaj   +1 more source

Simian virus 40 DNA replication in vitro: identification of multiple stages of initiation [PDF]

, 1989
A cell-free DNA replication system dependent upon five purified cellular proteins, one crude cellular fraction, and the simian virus 40 (SV40)-encoded large tumor antigen (T antigen) initiated and completed replication of plasmids containing the SV40 ...
Tsurimoto, T., Fairman, M. P., Stillman, B.   +2 more
core   +1 more source

E2 protein is the major determinant of specificity at the human papillomavirus origin of replication.

PLoS ONE, 2019
The replication of human papillomavirus (HPV) genomes requires E1 and E2 proteins as the viral trans-factors and the replication origin, located in the URR, as a cis-element.
Airiin Laaneväli, Mart Ustav, Ene Ustav, Marko Piirsoo   +3 more
doaj   +1 more source

RETRACTED: The structure of SV40 large T hexameric helicase in complex with AT-rich origin DNA

eLife, 2016
DNA replication is a fundamental biological process. The initial step in eukaryotic DNA replication is the assembly of the pre-initiation complex, including the formation of two head-to-head hexameric helicases around the replication origin.
Dahai Gai, Damian Wang, Shu-Xing Li, Xiaojiang S Chen   +3 more
doaj   +1 more source

Disintegration of Nascent Replication Bubbles during Thymine Starvation Triggers RecA- and RecBCD-dependent Replication Origin Destruction*

Journal of Biological Chemistry, 2012
Background: Thymine starvation causes irreversible toxicity and chromosomal damage, by unknown mechanisms. Results: Replication in thymine-starved cells leads to preferential fragmentation and degradation of new DNA and culminates in RecA-dependent ...
Kawai J. Kuong, A. Kuzminov
semanticscholar   +1 more source

The level of origin firing inversely affects the rate of replication fork progression [PDF]

, 2013
DNA damage slows DNA synthesis at replication forks; however, the mechanisms remain unclear. Cdc7 kinase is required for replication origin activation, is a target of the intra-S checkpoint, and is implicated in the response to replication fork stress ...
Aparicio, O. M., Jancuska, J. M., Knott, S. R., Nellimoottil, T., Peace, J. M., Rege, S., Sclafani, R. A., Tavare, S., Tecklenburg, M., Villwock, S. K., Yee, J. M., Zhong, Y.   +11 more
core   +1 more source

High-Resolution Profiling of Drosophila Replication Start Sites Reveals a DNA Shape and Chromatin Signature of Metazoan Origins

Cell Reports, 2015
At every cell cycle, faithful inheritance of metazoan genomes requires the concerted activation of thousands of DNA replication origins. However, the genetic and chromatin features defining metazoan replication start sites remain largely unknown.
Federico Comoglio, Tommy Schlumpf, Virginia Schmid, Remo Rohs, Christian Beisel, Renato Paro   +5 more
doaj   +1 more source

Rif1 is a global regulator of timing of replication origin firing in fission yeast.

Genes & Development, 2012
One of the long-standing questions in eukaryotic DNA replication is the mechanisms that determine where and when a particular segment of the genome is replicated.
Motoshi Hayano, Yutaka Kanoh, S. Matsumoto, Claire Renard-Guillet, K. Shirahige, H. Masai   +5 more
semanticscholar   +1 more source

Most human DNA replication initiation is dispersed throughout the genome with only a minority within previously identified initiation zones

Genome Biology
Background The identification of sites of DNA replication initiation in mammalian cells has been challenging. Here, we present unbiased detection of replication initiation events in human cells using BrdU incorporation and single-molecule nanopore ...
Jamie T. Carrington, Rosemary H. C. Wilson, Eduardo de La Vega, Sathish Thiyagarajan, Tom Barker, Leah Catchpole, Alex Durrant, Vanda Knitlhoffer, Chris Watkins, Karim Gharbi, Conrad A. Nieduszynski   +10 more
doaj   +1 more source

Unreplicated DNA remaining from unperturbed S phases passes through mitosis for resolution in daughter cells [PDF]

, 2016
To prevent rereplication of genomic segments, the eukaryotic cell cycle is divided into two nonoverlapping phases. During late mitosis and G1 replication origins are “licensed” by loading MCM2-7 double hexamers and during S phase licensed replication ...
Alberto Moreno, Eirini-Stavroula Komseli, Emma J. Haagensen, J. Julian Blow, Jamie T. Carrington, Luca Albergante, Mohammed Al Mamun, Petrakis, Timothy J. Newman, Vassilis G. Gorgoulis   +9 more
core   +2 more sources