Results 181 to 190 of about 1,971,949 (299)

Transcription at an inducible common fragile site reveals replication origin strength hierarchy. [PDF]

Nucleic Acids Res
Mandelbrojt J, Tonnerre-Doncarli C, Baret T, Masson A, Debatisse M, Prioleau MN.   +5 more
europepmc   +1 more source

Single-stranded DNA recruitment mechanism in replication origin unwinding by DnaA initiator protein and HU, an evolutionary ubiquitous nucleoid protein. [PDF]

Nucleic Acids Res, 2023
Yoshida R, Ozaki S, Kawakami H, Katayama T.   +3 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Determination of human DNA replication origin position and efficiency reveals principles of initiation zone organisation. [PDF]

Nucleic Acids Res, 2022
Guilbaud G, Murat P, Wilkes HS, Lerner LK, Sale JE, Krude T.   +5 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

MCM5 UFMylation regulates replication origin firing and fork progression. [PDF]

EMBO J
Li Z, Wu X, Liu L, Rao S, Liao Y, Liu M, Peng B, Zhang Q, Xia Y, Zhai Y, Takeda S, Xu X.   +11 more
europepmc   +1 more source

Dimerization of Firing Factors for Replication Origin Activation in Eukaryotes: A Crucial Process for Simultaneous Assembly of Bidirectional Replication Forks? [PDF]

Biology (Basel), 2022
Tanaka S, Ogawa S.
europepmc   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

AlphaFold-guided phylogenetic analyses suggest surprising heterogeneity in metazoan replication origin licensing mechanisms. [PDF]

EMBO J
Hunker O, Bleichert F.
europepmc   +1 more source

Refining the domain architecture model of the replication origin firing factor Treslin/TICRR. [PDF]

Life Sci Alliance, 2022
Ferreira P, Sanchez-Pulido L, Marko A, Ponting CP, Boos D.   +4 more
europepmc   +1 more source