Results 201 to 210 of about 247,162 (311)

AlphaFold-guided phylogenetic analyses suggest surprising heterogeneity in metazoan replication origin licensing mechanisms. [PDF]

EMBO J
Hunker O, Bleichert F.
europepmc   +1 more source

Refining the domain architecture model of the replication origin firing factor Treslin/TICRR. [PDF]

Life Sci Alliance, 2022
Ferreira P, Sanchez-Pulido L, Marko A, Ponting CP, Boos D.   +4 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

An Orc6 tether mediates ORC binding-site switching during replication origin licensing. [PDF]

Proc Natl Acad Sci U S A
Driscoll D, Friedman LJ, Gelles J, Bell SP.   +3 more
europepmc   +1 more source

MTBP phosphorylation controls DNA replication origin firing. [PDF]

Sci Rep, 2021
Ferreira P, Höfer V, Kronshage N, Marko A, Reusswig KU, Tetik B, Dießel C, Köhler K, Tschernoster N, Altmüller J, Schulze N, Pfander B, Boos D.   +12 more
europepmc   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

OriV-Finder: a comprehensive web server for bacterial plasmid replication origin analysis. [PDF]

Nucleic Acids Res
Li Y, Gao F.
europepmc   +1 more source

Mechanism of transcription initiation and primer generation at the mitochondrial replication origin OriL. [PDF]

EMBO J, 2021
Sarfallah A, Zamudio-Ochoa A, Anikin M, Temiakov D.   +3 more
europepmc   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

An additional replication origin causes cell cycle specific DNA replication fork speed. [PDF]

Front Microbiol
Skovgaard O.
europepmc   +1 more source