Results 191 to 200 of about 1,971,949 (299)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

An Orc6 tether mediates ORC binding-site switching during replication origin licensing. [PDF]

Proc Natl Acad Sci U S A
Driscoll D, Friedman LJ, Gelles J, Bell SP.   +3 more
europepmc   +1 more source

MTBP phosphorylation controls DNA replication origin firing. [PDF]

Sci Rep, 2021
Ferreira P, Höfer V, Kronshage N, Marko A, Reusswig KU, Tetik B, Dießel C, Köhler K, Tschernoster N, Altmüller J, Schulze N, Pfander B, Boos D.   +12 more
europepmc   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

OriV-Finder: a comprehensive web server for bacterial plasmid replication origin analysis. [PDF]

Nucleic Acids Res
Li Y, Gao F.
europepmc   +1 more source

Mechanism of transcription initiation and primer generation at the mitochondrial replication origin OriL. [PDF]

EMBO J, 2021
Sarfallah A, Zamudio-Ochoa A, Anikin M, Temiakov D.   +3 more
europepmc   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

An additional replication origin causes cell cycle specific DNA replication fork speed. [PDF]

Front Microbiol
Skovgaard O.
europepmc   +1 more source

Organization of DNA Replication Origin Firing in Xenopus Egg Extracts: The Role of Intra-S Checkpoint. [PDF]

Genes (Basel), 2021
Ciardo D, Haccard O, Narassimprakash H, Arbona JM, Hyrien O, Audit B, Marheineke K, Goldar A.   +7 more
europepmc   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source