Results 51 to 60 of about 89,500 (322)
A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
FEBS Letters, EarlyView.We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
wiley +1 more source
IEEE Access, 2023 Agile software development does not include elicitation and management of non-functional requirements (NFRs). Several techniques have been proposed by researchers to elicit, manage and prioritize NFRs.
Abdulhafeez Muhammad +4 more
doaj +1 more source
Agree to Disagree: Security Requirements Are Different, But Mechanisms For Security Adaptation Are Not [PDF]
, 2018 We describe a dialogue between a proponent and an opponent of the proposition "security is not just another quality attribute in self-adaptive systems". The dialogue is structured in two steps.
Bennaceur, Amel, Tun, Thein
core +1 more source
By dawn or dusk—how circadian timing rewrites bacterial infection outcomes
FEBS Letters, EarlyView.The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo +2 more
wiley +1 more source
Influence of Context on Decision Making during Requirements Elicitation [PDF]
, 2012 Requirements engineers should strive to get a better insight into decision making processes. During elicitation of requirements, decision making influences how stakeholders communicate with engineers, thereby affecting the engineers' understanding of ...
Burnay, Corentin +2 more
core +2 more sources
ELICA: An Automated Tool for Dynamic Extraction of Requirements Relevant Information
, 2018 Requirements elicitation requires extensive knowledge and deep understanding of the problem domain where the final system will be situated. However, in many software development projects, analysts are required to elicit the requirements from an ...
Abad, Zahra Shakeri Hossein +3 more
core +1 more source
User-centred interface design for cross-language information retrieval [PDF]
, 2002 This paper reports on the user-centered design methodology and techniques used for the elicitation of user requirements and how these requirements informed the first phase of the user interface design for a Cross-Language Information Retrieval System ...
Beaulieu, M. +3 more
core +1 more source
Anticipating Requirements Changes-Using Futurology in Requirements Elicitation
International Journal of Information System Modeling and Design, 2012 It is well known that requirements changes in a later phase of software developments is a major source of software defects and costs. Thus, the need of techniques to control or reduce the amount of changes during software development projects. The authors advocate the use of foresight methods as a valuable input to requirements elicitation, with the ...
Pimentel, João Henrique +3 more
openaire +3 more sources
Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer
FEBS Letters, EarlyView.Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani +4 more
wiley +1 more source
A Wargame-Augmented Knowledge Elicitation Method for the Agile Development of Novel Systems
Systems, 2020 There are inherent difficulties in designing an effective Human–Machine Interface (HMI) for a first-of-its-kind system. Many leading cognitive research methods rely upon experts with prior experiences using the system and/or some type of existing mockups
Stephen L. Dorton +4 more
doaj +1 more source