Results 111 to 120 of about 20,403,332 (296)
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
FEBS Letters, EarlyView.This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel +13 more
wiley +1 more source
Immigrants and electoral enrolment: Do the numbers add up? [PDF]
: The electoral enrolment of immigrants is important for effective democracy. Electoral rolls also provide useful administrative data on the age, regional distribution, and intercensal movements of the eligible voting population.
Anne Henderson
core +1 more source
FEBS Letters, EarlyView.Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura +2 more
wiley +1 more source
Vignette Research: Research Methods
Vignette research is an innovative qualitative, narrative and phenomenological research methodology that has gained international recognition, sparking interest from a wide range of individuals and institutions in global contexts. Vignettes are concise narratives, which capture human experiences in real life settings.
Agostini, Evi +2 more
openaire +4 more sources
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source
LIBS of Low-Alloyed Lead Systems: Chemometric Data Processing and Quantitative Analysis
AnalyticaA probabilistic–deterministic design of experiments (PDDoE) approach was employed to optimize laser-induced breakdown spectroscopy (LIBS) parameters for the quantitative determination of minor components in lead-based alloys.
Vitaliy Fomin +8 more
doaj +1 more source
A register-based census: what is the potential for New Zealand? [PDF]
Some countries, including Finland and Denmark, are now conducting their population censuses using administrative data registers rather than a nationwide survey of households.
Christine Bycroft
core
Phonetic and Phonological Research Sharing Methods [PDF]
, 2017 This paper reviews the materials linguists use to compile and share research in the linguistic sub-fields of phonetics and phonology. It summarizes the content and purpose of major books, journal publications, and databases within these two fields ...
Coogan, Cory C
core +1 more source