Results 31 to 40 of about 226,996 (233)

Avoidance of recognition sites of restriction-modification systems is a widespread but not universal anti-restriction strategy of prokaryotic viruses

BMC Genomics, 2018
Background Restriction-modification (R-M) systems protect bacteria and archaea from attacks by bacteriophages and archaeal viruses. An R-M system specifically recognizes short sites in foreign DNA and cleaves it, while such sites in the host DNA are ...
I. S. Rusinov, A. S. Ershova, A. S. Karyagina, S. A. Spirin, A. V. Alexeevski   +4 more
doaj   +1 more source

REMP software to introduce a screening REstriction site in site-directed Mutagenesis Primer

SoftwareX, 2021
Insertion of silent mutations allowing for restriction site modification aids in the screening of successful mutants during site directed mutagenesis. Introducing a new restriction site requires the analysis of degenerate sequences within mutant primer ...
Madhavi Latha Yadav Bangaru, PhD, Ravi Kumar Medabalimi, Sobhan Babu, PhD, Nidhanapati K. Raghavendra, PhD   +3 more
doaj   +1 more source

Organization of restriction-modification systems

Nucleic Acids Research, 1991
The genes for over 100 restriction-modification systems have now been cloned, and approximately one-half have been sequenced. Despite their similar function, they are exceedingly heterogeneous. The heterogeneity is evident at three levels: in the gene arrangements; in the enzyme compositions; and in the protein sequences. This paper summarizes the main
openaire   +3 more sources

Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering

Fermentation, 2023
Restriction endonucleases are a component of restriction–modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules.
Irina V. Alekseeva, Nikita A. Kuznetsov
doaj   +1 more source

Bacterial Autoimmunity Due to a Restriction-Modification System [PDF]

Current Biology, 2016
Restriction-modification (RM) systems represent a minimal and ubiquitous biological system of self/non-self discrimination in prokaryotes [1], which protects hosts from exogenous DNA [2]. The mechanism is based on the balance between methyltransferase (M) and cognate restriction endonuclease (R).
Pleška, Maroš, Qian, Long, Okura, Reiko, Bergmiller, Tobias, Wakamoto, Yuichi, Kussell, Edo, Guet, Călin C.   +6 more
openaire   +2 more sources

Transcription regulation of restriction-modification system Ecl18kI [PDF]

Nucleic Acids Research, 2009
Restriction-modification (R-M) system Ecl18kI is representative of R-M systems whose coordinated transcription is achieved through a separate DNA-binding domain of the methyltransferase. M.Ecl18kI recognizes an operator sequence located in the noncoding region that separates the divergently transcribed R and M genes.
Protsenko, Alexey, Zakharova, Marina, Nagornykh, Maxim, Solonin, Alexander, Severinov, Konstantin   +4 more
openaire   +2 more sources

Carcinomas and Carcinoid Tumors of the Lungs and Bronchi in Children and Adolescents: The EXPeRT Recommendations

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Primary lung carcinomas and bronchial carcinoid tumors (BC) are very rare malignancies in childhood. While typical BC and mucoepidermoid carcinomas are mostly low‐grade, localized tumors with a more favorable prognosis than in adults, necessitating avoidance of overtreatment, adenocarcinomas of the lung are often diagnosed at advanced disease ...
Michael Abele, Calogero Virgone, Michaela Kuhlen, Sabine Irtan, Yves Reguerre, Malgorzata Krawczyk, Ewa Bien, Tal Ben‐Ami, Maja Cesen, Ricardo Lopez‐Almaraz, Andrea Ferrari, Gianni Bisogno, Dominik T. Schneider, Abbas Agaimy, Constantin Lapa, Hubert Ducou le Pointe, Rainer Claus, Catherine Daniel, Ines B. Brecht, Daniel Orbach   +19 more
wiley   +1 more source

European Standard Clinical Practice Guideline and EXPeRT Recommendations for the Diagnosis and Management of Gastroenteropancreatic Neuroendocrine Neoplasms in Children and Adolescents

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Pediatric gastroenteropancreatic neuroendocrine neoplasms (GEP‐NENs) are extremely rare and clinically heterogeneous. Management has largely been extrapolated from adult practice. This European Standard Clinical Practice Guideline (ESCP), developed by the EXPeRT network in collaboration with adult NEN experts, provides (adult) evidence ...
Michaela Kuhlen, Calogero Virgone, Antje Redlich, Michael Abele, Ricardo Lopez‐Almaraz, Tal Ben‐Ami, Ewa Bien, Gianni Bisogno, Maja Mazic Cesen, Andrea Ferrari, Malgorzata A. Krawczyk, Yves Reguerre, Dominik T. Schneider, Ines B. Brecht, Charlotte Rigaud, Bilgehan Yalcin, Rainer Claus, Christian Vokuhl, Constantin Lapa, Jörg Fuchs, Segolene Hescot, Thomas Walter, Julien Hadoux, Daniel Orbach   +23 more
wiley   +1 more source

Cloning and sequence analysis of the Stsl restriction-modification gene: presence of homology to Fokl restriction-modification enzymes

Nucleic Acids Research, 1992
StsI endonuclease (R.StsI), a type IIs restriction endonuclease found in Streptococcus sanguis 54, recognizes the same sequence as FokI but cleaves at different positions. A DNA fragment that carried the genes for R.StsI and StsI methylase (M.StsI) was cloned from the chromosomal DNA of S.sanguis 54, and its nucleotide sequence was analyzed.
K, Kita, M, Suisha, H, Kotani, H, Yanase, N, Kato   +4 more
openaire   +3 more sources

Restriction Enzyme Recognition Sequence Search Program

BioTechniques, 2002
A critical and difficult part of characterizing restriction enzymes and methylases is the identification of recognition sequences. To simplify this process, we have developed a plasmid transformation method along with a computer program named RM search ...
Kyle P. Ellrott, Julie K.A. Kasarjian, Tao Jiang, Junichi Ryu   +3 more
doaj   +1 more source