Results 21 to 30 of about 226,996 (233)
BMC Evolutionary Biology, 2011 Background The discovery of restriction endonucleases and modification DNA methyltransferases, key instruments of genetic engineering, opened a new era of molecular biology through development of the recombinant DNA technology.
Solonin Alexander S +2 more
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Genetic manipulation of Staphylococci – Breaking through the barrier.
Frontiers in Cellular and Infection Microbiology, 2012 Most strains of Staphylococcus aureus and Staphylococcus epidermidis possess a strong restriction barrier that hinders exchange of DNA. Recently, major advances have been made in identifying and characterizing the restriction-modification systems ...
Ian R Monk, Timothy J Foster
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Cloning and Expression of the BalI Restriction-modification System [PDF]
Nucleic Acids Research, 1996 BalI, a type II restriction-modification (R-M) system from the bacterium, Brevibacterium albidum, recognizes the DNA sequence 5'-TGGCCA-3'. We cloned the genes encoding the BalI restriction endonuclease and methyltransferase and expressed them in Escherichia coli. The two genes were aligned tail-to-tail and their termination codons overlapped.
H, Ueno, I, Kato, Y, Ishino
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BMC Microbiology, 2008 Background Many microbes possess restriction-modification systems that protect them from parasitic DNA molecules. Unfortunately, the presence of a restriction-modification system in a given microbe also hampers genetic analysis.
Ward Jordan D +3 more
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Interplay Between Bacteriophages and Restriction-Modification Systems in Enterococci
Nova Biotechnologica et Chimica, 2014 The complete genomes of Enterococcus faecalis bacteriophages were analyzed for tetranucleotide words avoidance. Very similar tetranucleotide composition was found in all tested genomes with strong underrepresentation of palindromic GATC and GGCC words ...
Pristas Peter +2 more
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Restriction & Modification Enzymes
, 2022 Restriction and modification enzymes serve as nature’s genetic gatekeepers. These specialized proteins function together as a defence mechanism in bacteria and protect them from invading foreign DNA, such as that of bacteriophages. Restriction enzymes, also known as restriction endonucleases, recognize specific sequences within the DNA and cleave it at
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Transcription regulation of restriction-modification system Esp1396I [PDF]
Nucleic Acids Research, 2009 Abstract The convergently transcribed restriction (R) and methylase (M) genes of the Restriction–Modification system Esp1396I are tightly regulated by a controller (C) protein that forms part of the CR operon. We have mapped the transcriptional start sites from each promoter and examined the regulatory role of C.Esp1396I in vivo and in ...
Bogdanova, E. +6 more
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Frontiers in Cellular and Infection Microbiology, 2022 Anti-restriction proteins are typically encoded by plasmids, conjugative transposons, or phages to improve their chances of entering a new bacterial host with a type I DNA restriction and modification (RM) system.
Huimin Chen +6 more
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Type II restriction modification system in Ureaplasma parvum OMC-P162 strain. [PDF]
PLoS ONE, 2018 Ureaplasma parvum serovar 3 strain, OMC-P162, was isolated from the human placenta of a preterm delivery at 26 weeks' gestation. In this study, we sequenced the complete genome of OMC-P162 and compared it with other serovar 3 strains isolated from ...
Heng Ning Wu +8 more
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Domain movement within a gene: a novel evolutionary mechanism for protein diversification. [PDF]
PLoS ONE, 2011 A protein function is carried out by a specific domain localized at a specific position. In the present study, we report that, within a gene, a specific amino acid sequence can move between a certain position and another position.
Yoshikazu Furuta +3 more
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