A novel SfaNI-like restriction-modification system in Caldicellulosiruptor extents the genetic engineering toolbox for this genus. [PDF]
PLoS ONE, 2022 Caldicellulosiruptor is a genus of thermophilic to hyper-thermophilic microorganisms that express and secrete an arsenal of enzymes degrading lignocellulosic biomasses into fermentable sugars.
Steve Swinnen +7 more
doaj +5 more sources
An alternative approach to study the enzymatic specificities of the CfrBI restriction–modification system [PDF]
Heliyon, 2019 Restriction–modification systems (RMS) are the main gene-engineering tools and a suitable model to study the molecular mechanisms of catalysis and DNA–protein interactions.
Marina V. Zakharova +3 more
doaj +4 more sources
Characterization of the Type I Restriction Modification System Broadly Conserved among Group A Streptococci [PDF]
mSphere, 2021 Although prokaryotic DNA methylation investigations have long focused on immunity against exogenous DNA, it has been recently recognized that DNA methylation impacts gene expression and phase variation in Streptococcus pneumoniae and Streptococcus suis ...
Sruti DebRoy +8 more
doaj +3 more sources
A mobile restriction modification system consisting of methylases on the IncA/C plasmid [PDF]
Mobile DNA, 2019 Background IncA/C plasmids play important roles in the development and dissemination of multidrug resistance in bacteria. These plasmids carry three methylase genes, two of which show cytosine specificity.
Ruibai Wang, Jing Lou, Jie Li
doaj +4 more sources
Deletion of the Zinc Transporter Lipoprotein AdcAII Causes Hyperencapsulation of
mBio, 2020 The capsule is the dominant Streptococcus pneumoniae virulence factor, yet how variation in capsule thickness is regulated is poorly understood. Here, we describe an unexpected relationship between mutation of adcAII, which encodes a zinc uptake ...
Claire Durmort +11 more
doaj +3 more sources
The extremophile Picrophilus torridus carries a DNA adenine methylase M.PtoI that is part of a Type I restriction-modification system [PDF]
Frontiers in Microbiology, 2023 DNA methylation events mediated by orphan methyltransferases modulate various cellular processes like replication, repair and transcription. Bacteria and archaea also harbor DNA methyltransferases that are part of restriction-modification systems, which ...
Pallavi Gulati +3 more
doaj +2 more sources
Delineation of a lactococcal conjugation system reveals a restriction‐modification evasion system [PDF]
Microbial Biotechnology, 2023 Plasmid pUC11B is a 49.3‐kb plasmid harboured by the fermented meat isolate Lactococcus lactis subsp. lactis UC11. Among other features, pUC11B encodes a pMRC01‐like conjugation system and tetracycline‐resistance.
Guillermo Ortiz Charneco +8 more
doaj +2 more sources
The DNA Phosphorothioation Restriction-Modification System Influences the Antimicrobial Resistance of Pathogenic Bacteria [PDF]
Microbiology Spectrum, 2023 Bacterial defense barriers, such as DNA methylation-associated restriction-modification (R-M) and the CRISPR-Cas system, play an important role in bacterial antimicrobial resistance (AMR).
Congrui Xu +9 more
doaj +2 more sources
Possible existence of a system similar to bacterial restriction–modification in plants
AIMS Molecular Science, 2020 The bacterial restriction–modification (R-M) system has evolved as a defense mechanism against infectious phages and other types of integration of foreign DNA fragments into the host chromosome through recombination.
Larisa I. Fedoreyeva +1 more
doaj +2 more sources
The restriction impacts of the Type III restriction-modification system on the transmission dynamics of antimicrobial resistance genes in Campylobacter jejuni [PDF]
Frontiers in MicrobiologyIntroductionThe spread of antibiotic resistance genes among Campylobacter jejuni (C. jejuni) is a serious problem, and the effects of the restriction-modification (R-M) system on the transmission dynamics of these genes in C.
Yu Qiu +5 more
doaj +2 more sources