Deletion of the Zinc Transporter Lipoprotein AdcAII Causes Hyperencapsulation of
mBio, 2020 The capsule is the dominant Streptococcus pneumoniae virulence factor, yet how variation in capsule thickness is regulated is poorly understood. Here, we describe an unexpected relationship between mutation of adcAII, which encodes a zinc uptake ...
Claire Durmort +11 more
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A novel SfaNI-like restriction-modification system in Caldicellulosiruptor extents the genetic engineering toolbox for this genus [PDF]
PLoS ONE, 2022 Caldicellulosiruptor is a genus of thermophilic to hyper-thermophilic microorganisms that express and secrete an arsenal of enzymes degrading lignocellulosic biomasses into fermentable sugars.
Steve Swinnen +7 more
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IS-linked movement of a restriction-modification system. [PDF]
PLoS ONE, 2011 Potential mobility of restriction-modification systems has been suggested by evolutionary/bioinformatic analysis of prokaryotic genomes. Here we demonstrate in vivo movement of a restriction-modification system within a genome under a laboratory ...
Noriko Takahashi +4 more
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The Restriction–Modification Systems of Clostridium carboxidivorans P7 [PDF]
Microorganisms, 2023 Clostridium carboxidivorans P7 (DSM 15243) is a bacterium that converts syngas (a mixture of CO, H2, and CO2) into hexanol. An optimized and scaled-up industrial process could therefore provide a renewable source of fuels and chemicals while consuming ...
Patrick Kottenhahn +4 more
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An alternative approach to study the enzymatic specificities of the CfrBI restriction–modification system [PDF]
Heliyon, 2019 Restriction–modification systems (RMS) are the main gene-engineering tools and a suitable model to study the molecular mechanisms of catalysis and DNA–protein interactions.
Marina V. Zakharova +3 more
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Characterization of the Type I Restriction Modification System Broadly Conserved among Group A Streptococci [PDF]
mSphere, 2021 Although prokaryotic DNA methylation investigations have long focused on immunity against exogenous DNA, it has been recently recognized that DNA methylation impacts gene expression and phase variation in Streptococcus pneumoniae and Streptococcus suis ...
Sruti DebRoy +8 more
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A mobile restriction modification system consisting of methylases on the IncA/C plasmid [PDF]
Mobile DNA, 2019 Background IncA/C plasmids play important roles in the development and dissemination of multidrug resistance in bacteria. These plasmids carry three methylase genes, two of which show cytosine specificity.
Ruibai Wang, Jing Lou, Jie Li
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The extremophile Picrophilus torridus carries a DNA adenine methylase M.PtoI that is part of a Type I restriction-modification system [PDF]
Frontiers in Microbiology, 2023 DNA methylation events mediated by orphan methyltransferases modulate various cellular processes like replication, repair and transcription. Bacteria and archaea also harbor DNA methyltransferases that are part of restriction-modification systems, which ...
Pallavi Gulati +3 more
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Delineation of a lactococcal conjugation system reveals a restriction‐modification evasion system [PDF]
Microbial Biotechnology, 2023 Plasmid pUC11B is a 49.3‐kb plasmid harboured by the fermented meat isolate Lactococcus lactis subsp. lactis UC11. Among other features, pUC11B encodes a pMRC01‐like conjugation system and tetracycline‐resistance.
Guillermo Ortiz Charneco +8 more
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The DNA Phosphorothioation Restriction-Modification System Influences the Antimicrobial Resistance of Pathogenic Bacteria [PDF]
Microbiology Spectrum, 2023 Bacterial defense barriers, such as DNA methylation-associated restriction-modification (R-M) and the CRISPR-Cas system, play an important role in bacterial antimicrobial resistance (AMR).
Congrui Xu +9 more
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