Results 51 to 60 of about 728,418 (291)

Genetic manipulation of Staphylococci – Breaking through the barrier.

Frontiers in Cellular and Infection Microbiology, 2012
Most strains of Staphylococcus aureus and Staphylococcus epidermidis possess a strong restriction barrier that hinders exchange of DNA. Recently, major advances have been made in identifying and characterizing the restriction-modification systems ...
Ian R Monk, Timothy J Foster
doaj   +1 more source

Reassembling a cannon in the DNA defense arsenal: Genetics of StySA, a BREX phage exclusion system in Salmonella lab strains.

PLoS Genetics, 2022
Understanding mechanisms that shape horizontal exchange in prokaryotes is a key problem in biology. A major limit on DNA entry is imposed by restriction-modification (RM) processes that depend on the pattern of DNA modification at host-specified sites ...
Julie Zaworski, Oyut Dagva, Julius Brandt, Chloé Baum, Laurence Ettwiller, Alexey Fomenkov, Elisabeth A Raleigh   +6 more
doaj   +1 more source

Transcription regulation of restriction-modification system Esp1396I [PDF]

Nucleic Acids Research, 2009
Abstract The convergently transcribed restriction (R) and methylase (M) genes of the Restriction–Modification system Esp1396I are tightly regulated by a controller (C) protein that forms part of the CR operon. We have mapped the transcriptional start sites from each promoter and examined the regulatory role of C.Esp1396I in vivo and in ...
Bogdanova, E., Zakharova, M., Streeter, Simon, Taylor, J., Heyduk, T., Kneale, Geoff, Severinov, K.   +6 more
openaire   +3 more sources

Fused eco29kIR- and M genes coding for a fully functional hybrid polypeptide as a model of molecular evolution of restriction-modification systems

BMC Evolutionary Biology, 2011
Background The discovery of restriction endonucleases and modification DNA methyltransferases, key instruments of genetic engineering, opened a new era of molecular biology through development of the recombinant DNA technology.
Solonin Alexander S, Mokrishcheva Marina L, Nikitin Dmitri V   +2 more
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DNA methylation assessed by SMRT sequencing is linked to mutations in Neisseria meningitidis isolates [PDF]

, 2015
The Gram-negative bacterium Neisseria meningitidis features extensive genetic variability. To present, proposed virulence genotypes are also detected in isolates from asymptomatic carriers, indicating more complex mechanisms underlying variable ...
Gerd Pluschke, Christoph D. Schmid (72046), Schmid, Christoph D., Mane, Shrikant, Korlach, Jonas, Tyson A. Clark (105783), Katharina Röltgen, Wang, Guilin, Araceli Lamelas, Jonas Korlach (105788), Shrikant Mane, Araceli Lamelas (128475), Christoph D Schmid, Mohamad R Abdul Sater, Clark, Tyson A., Röltgen, Katharina, Gerd Pluschke (7855), Katharina Röltgen (120045), Guilin Wang, Sater, Mohamad R. Abdul, Tyson A Clark, Mohamad R. Abdul Sater (836669), Jonas Korlach, Guilin Wang (37620), Shrikant Mane (106631), Lamelas, Araceli, Pluschke, Gerd   +26 more
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Transcription regulation of restriction-modification system Ecl18kI [PDF]

Nucleic Acids Research, 2009
Restriction-modification (R-M) system Ecl18kI is representative of R-M systems whose coordinated transcription is achieved through a separate DNA-binding domain of the methyltransferase. M.Ecl18kI recognizes an operator sequence located in the noncoding region that separates the divergently transcribed R and M genes.
Protsenko, Alexey, Zakharova, Marina, Nagornykh, Maxim, Solonin, Alexander, Severinov, Konstantin   +4 more
openaire   +2 more sources

Generation of a restriction minus enteropathogenic Escherichia coli E2348/69 strain that is efficiently transformed with large, low copy plasmids

BMC Microbiology, 2008
Background Many microbes possess restriction-modification systems that protect them from parasitic DNA molecules. Unfortunately, the presence of a restriction-modification system in a given microbe also hampers genetic analysis.
Ward Jordan D, Price Nancy L, Hobson Neil, Raivio Tracy L   +3 more
doaj   +1 more source

Domain movement within a gene: a novel evolutionary mechanism for protein diversification. [PDF]

PLoS ONE, 2011
A protein function is carried out by a specific domain localized at a specific position. In the present study, we report that, within a gene, a specific amino acid sequence can move between a certain position and another position.
Yoshikazu Furuta, Mikihiko Kawai, Ikuo Uchiyama, Ichizo Kobayashi   +3 more
doaj   +1 more source

A DNA phosphorothioation-based Dnd defense system provides resistance against various phages and is compatible with the Ssp defense system

mBio, 2023
DndABCDE-catalyzed DNA phosphorothioation (PT), in which the nonbridging oxygen is swapped with a sulfur atom, was first identified in the bacterial genome.
Susu Jiang, Ke Chen, Yingying Wang, Yueying Zhang, Yaru Tang, Wanqiu Huang, Xiaolin Xiong, Shi Chen, Chao Chen, Lianrong Wang   +9 more
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Adaptative Potential of the Lactococcus Lactis IL594 Strain Encoded in Its 7 Plasmids [PDF]

, 2011
The extrachromosomal gene pool plays a significant role both in evolution and in the environmental adaptation of bacteria. The L. lactis subsp. lactis IL594 strain contains seven plasmids, named pIL1 to pIL7, and is the parental strain of the plasmid ...
Roman K Górecki, Marcin Grynberg, Górecki Roman K., Grynberg Marcin, Koryszewska-Bagińska, Anna, Marcin Gołębiewski, Jacek K Bardowski, Roman K. Górecki, Grynberg, Marcin, Gołębiewski, Marcin, Żylińska Joanna, Żylińska, Joanna, Górecki, Roman Krzysztof, Bardowski Jacek K., Anna Koryszewska-Bagińska, Bardowski, Jacek K., Joanna Żylińska, Jacek K. Bardowski, Gołębiewski Marcin, Koryszewska-Bagińska Anna   +19 more
core   +1 more source