REtools: A laboratory program for restriction enzyme work: enzyme selection and reaction condition assistance [PDF]
BMC Bioinformatics, 2006 Background Restriction enzymes are one of the everyday tools used in molecular biology. The continuously expanding panel of known restriction enzymes (several thousands) renders their optimal use virtually impossible without computerized assistance ...
Boulukos Kim E +2 more
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MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme. [PDF]
Nucleic Acids Res, 2018 Efficient DNA assembly is of great value in biological research and biotechnology. Type IIS restriction enzyme-based assembly systems allow assembly of multiple DNA fragments in a one-pot reaction.
Lin D, O'Callaghan CA.
europepmc +2 more sources
A solution to the subdiffusion-efficiency paradox: Inactive states enhance reaction efficiency at subdiffusion conditions in living cells [PDF]
EPL 97, 20008 (2012), 2012 Macromolecular crowding in living biological cells effects subdiffusion of larger biomolecules such as proteins and enzymes. Mimicking this subdiffusion in terms of random walks on a critical percolation cluster, we here present a case study of EcoRV ...
Lomholt, Michael A. +2 more
core +2 more sources
Laboratory Exercise To Measure Restriction Enzyme Kinetics [PDF]
Journal of Microbiology & Biology Education, 2019 Enzymes are ubiquitous in the fields of biology and microbiology, catalyzing critical reactions and enabling a broad range of biotechnological applications.
Caroline Blassick +4 more
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REBASE–restriction enzymes and methylases [PDF]
Nucleic Acids Research, 1993 REBASE is a comprehensive database of information about restriction enzymes and related proteins. It contains published and unpublished references, recognition and cleavage sites, isoschizomers, commercial availability, methylation sensitivity, crystal and sequence data.
Richard J. Roberts, Dana Macelis
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Characterization of eukaryotic DNA N(6)-methyladenine by a highly sensitive restriction enzyme-assisted sequencing. [PDF]
Nat Commun, 2016 Although extensively studied in prokaryotes, the prevalence and significance of DNA N6-methyladenine (6mA or m6dA) in eukaryotes had been underappreciated until recent studies, which have demonstrated that 6mA regulates gene expression as a potential ...
Luo GZ +8 more
europepmc +2 more sources
Using shotgun sequence data to find active restriction enzyme genes [PDF]
Nucleic Acids Research, 2009 Whole genome shotgun sequence analysis has become the standard method for beginning to determine a genome sequence. The preparation of the shotgun sequence clones is, in fact, a biological experiment.
Baer +33 more
core +3 more sources
Alteration of sequence specificity of the type IIS restriction endonuclease BtsI. [PDF]
PLoS ONE, 2010 The Type IIS restriction endonuclease BtsI recognizes and digests at GCAGTG(2/0). It comprises two subunits: BtsIA and BtsIB. The BtsIB subunit contains the recognition domain, one catalytic domain for bottom strand nicking and part of the catalytic ...
Shengxi Guan +3 more
doaj +12 more sources
Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA. [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 1992 Introduction of restriction enzyme along with linearized plasmid results in integration of plasmid DNA at genomic restriction sites in a high proportion of the resulting transformants.
Adam Kuspa, William F. Loomis
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