Results 1 to 10 of about 3,173,233 (370)
MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme. [PDF]
Nucleic Acids Res, 2018 Efficient DNA assembly is of great value in biological research and biotechnology. Type IIS restriction enzyme-based assembly systems allow assembly of multiple DNA fragments in a one-pot reaction.
Lin D, O'Callaghan CA.
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Laboratory Exercise To Measure Restriction Enzyme Kinetics [PDF]
Journal of Microbiology & Biology Education, 2019 Enzymes are ubiquitous in the fields of biology and microbiology, catalyzing critical reactions and enabling a broad range of biotechnological applications.
Caroline Blassick +4 more
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REBASE–restriction enzymes and methylases [PDF]
Nucleic Acids Research, 1993 REBASE is a comprehensive database of information about restriction enzymes and related proteins. It contains published and unpublished references, recognition and cleavage sites, isoschizomers, commercial availability, methylation sensitivity, crystal and sequence data.
Richard J. Roberts, Dana Macelis
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Characterization of eukaryotic DNA N(6)-methyladenine by a highly sensitive restriction enzyme-assisted sequencing. [PDF]
Nat Commun, 2016 Although extensively studied in prokaryotes, the prevalence and significance of DNA N6-methyladenine (6mA or m6dA) in eukaryotes had been underappreciated until recent studies, which have demonstrated that 6mA regulates gene expression as a potential ...
Luo GZ +8 more
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Genomic-Based Restriction Enzyme Selection for Specific Detection of Piscirickettsia salmonis by 16S rDNA PCR-RFLP. [PDF]
Front Microbiol, 2016 The gram negative facultative bacterium P. salmonis is the etiological agent of Salmonid Rickettsial Septicaemia (SRS), a severe disease that causes important economic losses in the global salmon farmer industry.
Mandakovic D +6 more
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SNP-RFLPing: restriction enzyme mining for SNPs in genomes
BMC Genomics, 2006 Background The restriction fragment length polymorphism (RFLP) is a common laboratory method for the genotyping of single nucleotide polymorphisms (SNPs).
Cheng Yu-Huei +4 more
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Restriction enzymes and their isoschizomers [PDF]
Nucleic Acids Research, 1991 Richard J. Roberts, D. Macelis
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Cold Spring Harbor Protocols, 2021 Restriction enzymes provided the foundation on which molecular cloning was built, and they remain as essential tools in current recombinant DNA technology. The three classes of restriction enzymes and their features are introduced here.
Michael R, Green, Joseph, Sambrook
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Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA. [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 1992 Adam Kuspa, William F. Loomis
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