Restriction Enzyme Recognition Sequence Search Program [PDF]
BioTechniques, 2002 A critical and difficult part of characterizing restriction enzymes and methylases is the identification of recognition sequences. To simplify this process, we have developed a plasmid transformation method along with a computer program named RM search ...
Kyle P. Ellrott +3 more
doaj +2 more sources
Laboratory Exercise To Measure Restriction Enzyme Kinetics [PDF]
Journal of Microbiology & Biology Education, 2019 Enzymes are ubiquitous in the fields of biology and microbiology, catalyzing critical reactions and enabling a broad range of biotechnological applications.
Caroline Blassick +4 more
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Evaluating restriction enzyme selection for reduced representation sequencing in conservation genomics. [PDF]
Mol Ecol Resour, 2023 López A +3 more
europepmc +3 more sources
Short-range translocation by a restriction enzyme motor triggers diffusion along DNA. [PDF]
Nat Chem BiolGöse M +8 more
europepmc +3 more sources
A Restriction Enzyme from Escherichia coli Purification and General Properties [PDF]
Engineering and Technology Journal, 2009 An endonuclease restriction enzyme has been purified from E. coli about 40-fold withDNAse and RNAse recoveries of about 3%. The purification steps included precipitationof the enzyme with ammonium sulphate, and reclaimed it through Sephadex G-100 andDEAE-
Mukaram Shikara +2 more
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The Restriction–Modification Systems of Clostridium carboxidivorans P7
Microorganisms, 2023 Clostridium carboxidivorans P7 (DSM 15243) is a bacterium that converts syngas (a mixture of CO, H2, and CO2) into hexanol. An optimized and scaled-up industrial process could therefore provide a renewable source of fuels and chemicals while consuming ...
Patrick Kottenhahn +4 more
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Restriction fragment length polymorphism analysis of genes of virulent strain isolate of Toxoplasma gondii using enzyme DdeI [PDF]
International Journal of One Health, 2021 Background and Aim: Toxoplasma gondii is a unicellular coccidian parasite distributed globally and is an important zoonotic pathogen. Approximately 30% of the human population worldwide is chronically infected with T. gondii.
Fitrine Ekawasti +6 more
doaj +1 more source
User-Friendly Reverse Genetics System for Modification of the Right End of Fowl Adenovirus 4 Genome
Viruses, 2020 A novel fowl adenovirus 4 (FAdV-4) has caused significant economic losses to the poultry industry in China since 2015. We established an easy-to-use reverse genetics system for modification of the whole right and partial left ends of the novel FAdV-4 ...
Bingyu Yan +8 more
doaj +1 more source
Resolution of Joint Molecules by RuvABC and RecG Following Cleavage of the Escherichia coli Chromosome by EcoKI [PDF]
, 2009 DNA double-strand breaks can be repaired by homologous recombination involving the formation and resolution of Holliday junctions. In Escherichia coli, the RuvABC resolvasome and the RecG branch-migration enzyme have been proposed to act in alternative ...
Leach, David +2 more
core +6 more sources
Altering under-represented DNA sequences elevates bacterial transformation efficiency
mBio, 2023 A cornerstone of bacterial molecular biology is the ability to genetically manipulate the microbe under study. Many bacteria are difficult to manipulate genetically, a phenotype due in part to robust removal of newly acquired DNA, for example, by ...
Shuai Hu +5 more
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