REtools: A laboratory program for restriction enzyme work: enzyme selection and reaction condition assistance [PDF]
BMC Bioinformatics, 2006 Background Restriction enzymes are one of the everyday tools used in molecular biology. The continuously expanding panel of known restriction enzymes (several thousands) renders their optimal use virtually impossible without computerized assistance ...
Boulukos Kim E +2 more
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Laboratory Exercise To Measure Restriction Enzyme Kinetics [PDF]
Journal of Microbiology & Biology Education, 2019 Enzymes are ubiquitous in the fields of biology and microbiology, catalyzing critical reactions and enabling a broad range of biotechnological applications.
Caroline Blassick +4 more
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SNP-RFLPing 2: an updated and integrated PCR-RFLP tool for SNP genotyping [PDF]
BMC Bioinformatics, 2010 Background PCR-restriction fragment length polymorphism (RFLP) assay is a cost-effective method for SNP genotyping and mutation detection, but the manual mining for restriction enzyme sites is challenging and cumbersome.
Chang Hsueh-Wei +3 more
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A Restriction Enzyme from Escherichia coli Purification and General Properties [PDF]
Engineering and Technology Journal, 2009 An endonuclease restriction enzyme has been purified from E. coli about 40-fold withDNAse and RNAse recoveries of about 3%. The purification steps included precipitationof the enzyme with ammonium sulphate, and reclaimed it through Sephadex G-100 andDEAE-
Mukaram Shikara +2 more
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The Restriction–Modification Systems of Clostridium carboxidivorans P7
Microorganisms, 2023 Clostridium carboxidivorans P7 (DSM 15243) is a bacterium that converts syngas (a mixture of CO, H2, and CO2) into hexanol. An optimized and scaled-up industrial process could therefore provide a renewable source of fuels and chemicals while consuming ...
Patrick Kottenhahn +4 more
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Restriction fragment length polymorphism analysis of genes of virulent strain isolate of Toxoplasma gondii using enzyme DdeI [PDF]
International Journal of One Health, 2021 Background and Aim: Toxoplasma gondii is a unicellular coccidian parasite distributed globally and is an important zoonotic pathogen. Approximately 30% of the human population worldwide is chronically infected with T. gondii.
Fitrine Ekawasti +6 more
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User-Friendly Reverse Genetics System for Modification of the Right End of Fowl Adenovirus 4 Genome
Viruses, 2020 A novel fowl adenovirus 4 (FAdV-4) has caused significant economic losses to the poultry industry in China since 2015. We established an easy-to-use reverse genetics system for modification of the whole right and partial left ends of the novel FAdV-4 ...
Bingyu Yan +8 more
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Altering under-represented DNA sequences elevates bacterial transformation efficiency
mBio, 2023 A cornerstone of bacterial molecular biology is the ability to genetically manipulate the microbe under study. Many bacteria are difficult to manipulate genetically, a phenotype due in part to robust removal of newly acquired DNA, for example, by ...
Shuai Hu +5 more
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A method for identifying allele-specific hydroxymethylation
Epigenetics, 2020 We previously identified sequence-dependent allele-specific methylation (sd-ASM) in adult human peripheral blood leukocytes, in which ASM occurs in cis depending on adjacent polymorphic sequences.
Yoichi Yamada, Sho Sasaki
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Restriction Enzyme Recognition Sequence Search Program
BioTechniques, 2002 A critical and difficult part of characterizing restriction enzymes and methylases is the identification of recognition sequences. To simplify this process, we have developed a plasmid transformation method along with a computer program named RM search ...
Kyle P. Ellrott +3 more
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