Results 181 to 190 of about 347,445 (313)

Structural and functional analysis of Bacillus sarcosine oxidase and its activity toward cyclic imino acids

FEBS Open Bio, EarlyView.
Sarcosine oxidase from Bacillus sp. (SoxB) recognizes l‐proline (l‐Pro), d‐proline (d‐Pro), and l‐thioproline (l‐Tpr) as minor substrates. In this study, we measured their absorption spectra, determined the crystal structures of their enzyme complexes, and performed site‐directed mutagenesis of key residues.
Yuqi Zhang, Yoshitaka Nakajima, Masae Kurobe, Tsutomu Nakamura, Tomoki Himiyama, Yoshiaki Nishiya   +5 more
wiley   +1 more source

Evaluating restriction enzyme selection for reduced representation sequencing in conservation genomics. [PDF]

Mol Ecol Resour
López A, Carreras C, Pascual M, Pegueroles C.   +3 more
europepmc   +1 more source

Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics

FEBS Open Bio, EarlyView.
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini, Nicolò Antonelli, Gianluca Molla   +2 more
wiley   +1 more source

Methylation‑sensitive restriction enzyme‑droplet digital PCR assay for the one‑step highly sensitive analysis of DNA methylation hotspots. [PDF]

Int J Mol Med
Gattuso G, Lavoro A, Caltabiano R, Madonna G, Capone M, Ascierto PA, Falzone L, Libra M, Candido S.   +8 more
europepmc   +1 more source

PCR-Restriction Enzyme Analysis for Detection of Candida DNA in Blood from Febrile Patients with Hematological Malignancies [PDF]

, 1999
Giulia Morace, Livio Pagano, Maurizio Sanguinetti, Brunella Posteraro, Luca Mele, Francesco Equitani, Giuseppina D’Amore, Giuseppe Leone, Giovanni Fadda   +8 more
openalex   +1 more source

Interplay between RNA‐protein interactions and RNA structures in gene regulation

FEBS Open Bio, EarlyView.
Methodological advances in mapping transcriptome‐wide RNA‐protein interactions and RNA structures have started to uncover the potential of RNP conformations in gene regulation. Competing RNA–RNA, RNA‐protein and protein–protein interactions shape the compaction and function of RNPs throughout their lifetime and may provide novel therapeutic targets in ...
Jenni Rapakko, Mauro Scaravilli, Minna‐Liisa Änkö   +2 more
wiley   +1 more source

Importance of E484K and N501Y mutations in SARS-CoV-2 for genomic surveillance: rapid detection by restriction enzyme analysis

, 2021
Jaspe RC, Sulbaran Y, Loureiro CL, D Angelo P, Rodriguez L, Garzaro DJ, Rangel HR, Pujol FH.   +7 more
europepmc   +1 more source

Correction to: Dead Cas9-sgRNA Complex Shelters Vulnerable DNA Restriction Enzyme Sites from Cleavage for Cloning Applications, by Saifaldeen M et al. The CRISPR Journal, 2021;4(2):275-289; DOI: 10.1089/crispr.2020.0134. [PDF]

CRISPR J, 2021
europepmc   +1 more source

A ligation and restriction enzyme independent cloning technique: an alternative to conventional methods for cloning hard-to-clone gene segments in the influenza reverse genetics system. [PDF]

Virol J, 2020
Bhat S, Bialy D, Sealy JE, Sadeyen JR, Chang P, Iqbal M.   +5 more
europepmc   +1 more source

Tryptophan metabolite atlas uncovers organ, age, and sex‐specific variations

FEBS Open Bio, EarlyView.
Tryptophan metabolites were analyzed across twelve organs, the central nervous system, and serum in male and female mice at three life stages. We found tissue‐, sex‐, and age‐specific differences, including increased indole‐3‐pyruvate and kynurenine in aging males.
Lizbeth Perez‐Castro, Afshan F. Nawas, Jessica A. Kilgore, Pedro A. S. Nogueira, M.Carmen Lafita‐Navarro, Paul H. Acosta, Roy Garcia, Noelle S. Williams, Maralice Conacci‐Sorrell   +8 more
wiley   +1 more source