Results 11 to 20 of about 347,445 (313)
Restriction enzymes and their isoschizomers [PDF]
Nucleic Acids Research, 1987 Since the last compilation of restriction enzymes (1), 300 new entries have been added including 12 new specificities. With the growing size of this database and the recognition that the most widespread use of the information is as a database for computer programs predicting restriction enzyme cleavage patterns, the new format has been continued ...
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REBASE: restriction enzymes and methyltransferases [PDF]
Nucleic Acids Research, 2003 REBASE contains comprehensive information about restriction enzymes, DNA methyltransferases and related proteins such as nicking enzymes, specificity subunits and control proteins. It contains published and unpublished references, recognition and cleavage sites, isoschizomers, commercial availability, crystal and sequence data. Homing endonucleases are
Tamas Vincze +3 more
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A method for identifying allele-specific hydroxymethylation
Epigenetics, 2020 We previously identified sequence-dependent allele-specific methylation (sd-ASM) in adult human peripheral blood leukocytes, in which ASM occurs in cis depending on adjacent polymorphic sequences.
Yoichi Yamada, Sho Sasaki
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Optimized In Vitro Restriction Digestion Protocol for Preparing Maize and Barley ddRAD-Seq Libraries
Agronomy, 2023 In recent years, high-throughput sequencing methods have become increasingly popular in molecular biology laboratories, mainly due to the relatively low cost of small, benchtop platforms, the simplicity of library preparation, and the low price per unit ...
Marta Puchta-Jasińska +3 more
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The Architecture of Restriction Enzymes [PDF]
Structure, 2013 In this issue of Structure, Lyumkis and colleagues describe a high resolution structure of a polymerized form of the SgrAI restriction enzyme, which shows that it forms a helical assembly with four enzyme molecules per turn of the helix. The DNA is arranged on the periphery of the protein helix pointing away from the helical axis.
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Brazilian Archives of Biology and Technology, 2008 The presence of restriction enzymes in the transformation mixture improved the efficiency of transformation in Moniliophthora perniciosa. The influence of the vector shape (linear or circular), the patterns of plasmid integration in genomic sites and the
Francis Julio Fagundes Lopes +4 more
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A Sliding Restriction Enzyme Pauses [PDF]
Structure, 2007 In this issue of Structure, Aggarwal and colleagues (Townson et al., 2007) present the crystal structure of the restriction endonuclease BstYI in complex with a near-cognate substrate. This structure most likely reflects the conformation BstYI adopts as it scans DNA and pauses upon encountering a site similar to its recognition sequence.
Wolfgang Wende, Alfred Pingoud
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Biomolecular computers with multiple restriction enzymes [PDF]
Genetics and Molecular Biology, 2017 The development of conventional, silicon-based computers has several limitations, including some related to the Heisenberg uncertainty principle and the von Neumann "bottleneck". Biomolecular computers based on DNA and proteins are largely free of these disadvantages and, along with quantum computers, are reasonable alternatives to their conventional ...
Sakowski, Sebastian +5 more
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Scientific Papers Animal Science and Biotechnologies, 2023 BMP15-FecX is one of the major genes associated with prolificacy in different sheep breeds (Ovis aries), alongside BMPR15/FecB and GDF9/FecG. The majority of the polymorphisms, associated with ovulation rate in different breeds, are located in the exonic
Viorica Coşier
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BioTechniques, 2016 Directed evolution has greatly facilitated protein engineering and provided new insights into protein structure—function relationships. DNA shuffling using restriction enzymes is a particularly simple and cost-effective means of recombinatorial evolution
Weiliang Huang +3 more
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