A solution to the subdiffusion-efficiency paradox: Inactive states enhance reaction efficiency at subdiffusion conditions in living cells [PDF]
EPL 97, 20008 (2012), 2012 Macromolecular crowding in living biological cells effects subdiffusion of larger biomolecules such as proteins and enzymes. Mimicking this subdiffusion in terms of random walks on a critical percolation cluster, we here present a case study of EcoRV ...
Lomholt, Michael A. +2 more
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Laboratory Exercise To Measure Restriction Enzyme Kinetics [PDF]
Journal of Microbiology & Biology Education, 2019 Enzymes are ubiquitous in the fields of biology and microbiology, catalyzing critical reactions and enabling a broad range of biotechnological applications.
Caroline Blassick +4 more
doaj +2 more sources
Restriction enzymes and their isoschizomers [PDF]
Nucleic Acids Research, 1991 R J, Roberts, D, Macelis
openaire +6 more sources
The Restriction–Modification Systems of Clostridium carboxidivorans P7
Microorganisms, 2023 Clostridium carboxidivorans P7 (DSM 15243) is a bacterium that converts syngas (a mixture of CO, H2, and CO2) into hexanol. An optimized and scaled-up industrial process could therefore provide a renewable source of fuels and chemicals while consuming ...
Patrick Kottenhahn +4 more
doaj +1 more source
User-Friendly Reverse Genetics System for Modification of the Right End of Fowl Adenovirus 4 Genome
Viruses, 2020 A novel fowl adenovirus 4 (FAdV-4) has caused significant economic losses to the poultry industry in China since 2015. We established an easy-to-use reverse genetics system for modification of the whole right and partial left ends of the novel FAdV-4 ...
Bingyu Yan +8 more
doaj +1 more source
A Restriction Enzyme from Escherichia coli Purification and General Properties [PDF]
Engineering and Technology Journal, 2009 An endonuclease restriction enzyme has been purified from E. coli about 40-fold withDNAse and RNAse recoveries of about 3%. The purification steps included precipitationof the enzyme with ammonium sulphate, and reclaimed it through Sephadex G-100 andDEAE-
Mukaram Shikara +2 more
doaj +1 more source
Restriction fragment length polymorphism analysis of genes of virulent strain isolate of Toxoplasma gondii using enzyme DdeI [PDF]
International Journal of One Health, 2021 Background and Aim: Toxoplasma gondii is a unicellular coccidian parasite distributed globally and is an important zoonotic pathogen. Approximately 30% of the human population worldwide is chronically infected with T. gondii.
Fitrine Ekawasti +6 more
doaj +1 more source
Altering under-represented DNA sequences elevates bacterial transformation efficiency
mBio, 2023 A cornerstone of bacterial molecular biology is the ability to genetically manipulate the microbe under study. Many bacteria are difficult to manipulate genetically, a phenotype due in part to robust removal of newly acquired DNA, for example, by ...
Shuai Hu +5 more
doaj +1 more source
A method for identifying allele-specific hydroxymethylation
Epigenetics, 2020 We previously identified sequence-dependent allele-specific methylation (sd-ASM) in adult human peripheral blood leukocytes, in which ASM occurs in cis depending on adjacent polymorphic sequences.
Yoichi Yamada, Sho Sasaki
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Kinetics of target site localization of a protein on DNA: a stochastic approach [PDF]
, 2004 It is widely recognized that the cleaving rate of a restriction enzyme on target DNA sequences is several orders of magnitude faster than the maximal one calculated from the diffusion--limited theory.
Berg +21 more
core +3 more sources