Results 11 to 20 of about 2,927,350 (286)

A method for identifying allele-specific hydroxymethylation

Epigenetics, 2020
We previously identified sequence-dependent allele-specific methylation (sd-ASM) in adult human peripheral blood leukocytes, in which ASM occurs in cis depending on adjacent polymorphic sequences.
Yoichi Yamada, Sho Sasaki
doaj   +1 more source

Altering under-represented DNA sequences elevates bacterial transformation efficiency

mBio, 2023
A cornerstone of bacterial molecular biology is the ability to genetically manipulate the microbe under study. Many bacteria are difficult to manipulate genetically, a phenotype due in part to robust removal of newly acquired DNA, for example, by ...
Shuai Hu, Stefani Giacopazzi, Ryan Modlin, Kevin Karplus, David L. Bernick, Karen M. Ottemann   +5 more
doaj   +1 more source

Optimized In Vitro Restriction Digestion Protocol for Preparing Maize and Barley ddRAD-Seq Libraries

Agronomy, 2023
In recent years, high-throughput sequencing methods have become increasingly popular in molecular biology laboratories, mainly due to the relatively low cost of small, benchtop platforms, the simplicity of library preparation, and the low price per unit ...
Marta Puchta-Jasińska, Paulina Bolc, Urszula Piechota, Maja Boczkowska   +3 more
doaj   +1 more source

Genomic-Based Restriction Enzyme Selection for Specific Detection of Piscirickettsia salmonis by 16S rDNA PCR-RFLP

Frontiers in Microbiology, 2016
The gram negative facultative bacterium P. salmonis is the etiological agent of Salmonid Rickettsial Septicaemia (SRS), a severe disease that causes important economic losses in the global salmon farmer industry.
D. Mandakovic, B. Glasner, J. Maldonado, P. Aravena, Mauricio González, V. Cambiazo, R. Pulgar   +6 more
semanticscholar   +1 more source

Restriction Enzyme Recognition Sequence Search Program

BioTechniques, 2002
A critical and difficult part of characterizing restriction enzymes and methylases is the identification of recognition sequences. To simplify this process, we have developed a plasmid transformation method along with a computer program named RM search ...
Kyle P. Ellrott, Julie K.A. Kasarjian, Tao Jiang, Junichi Ryu   +3 more
doaj   +1 more source

Development of RFLP-PCR method for the identification of medically important Aspergillus species using single restriction enzyme MwoI

Brazilian Journal of Microbiology, 2014
In this study we attempted to modify the PCR-RFLP method using restriction enzyme MwoI for the identification of medically important Aspergillus species.
K. Diba, Hossein Mirhendi, P. Kordbacheh, S. Rezaie   +3 more
semanticscholar   +1 more source

HinfI Polymorphisms Characterization in the 1st intron of BMP15/FecX Gene in Turcana and Tsigai Sheep (Ovis aries)

Scientific Papers Animal Science and Biotechnologies, 2023
BMP15-FecX is one of the major genes associated with prolificacy in different sheep breeds (Ovis aries), alongside BMPR15/FecB and GDF9/FecG. The majority of the polymorphisms, associated with ovulation rate in different breeds, are located in the exonic
Viorica Coşier
doaj  

Restriction Enzyme Digestion Exercise – An In-class Activity

Journal of Microbiology & Biology Education, 2010
Understanding the concepts of molecular biology and then applying those concepts to laboratory experiments can be challenging to entry-level students. In order to facilitate the topics of restriction enzyme digestion and the generation of compatible ends
Michelle Parent
doaj   +1 more source

Estimating absolute methylation levels at single-CpG resolution from methylation enrichment and restriction enzyme sequencing methods

Annual International Conference on Research in Computational Molecular Biology, 2013
Recent advancements in sequencing-based DNA methylation profiling methods provide an unprecedented opportunity to map complete DNA methylomes. These include whole-genome bisulfite sequencing (WGBS, MethylC-seq, or BS-seq), reduced-representation ...
Michael Stevens, Jeffrey B. Cheng, Mingchao Xie, J. Costello, Ting Wang   +4 more
semanticscholar   +1 more source

REtools: A laboratory program for restriction enzyme work: enzyme selection and reaction condition assistance

BMC Bioinformatics, 2006
Background Restriction enzymes are one of the everyday tools used in molecular biology. The continuously expanding panel of known restriction enzymes (several thousands) renders their optimal use virtually impossible without computerized assistance ...
Boulukos Kim E, Martin Patrick, Pognonec Philippe   +2 more
doaj   +1 more source