Cold Spring Harbor Protocols, 2021 Restriction enzymes provided the foundation on which molecular cloning was built, and they remain as essential tools in current recombinant DNA technology. The three classes of restriction enzymes and their features are introduced here.
Michael R, Green, Joseph, Sambrook
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REBASE--restriction enzymes and methylases [PDF]
Nucleic Acids Research, 1993 REBASE is a comprehensive database of information about restriction enzymes and related proteins. It contains published and unpublished references, recognition and cleavage sites, isoschizomers, commercial availability, methylation sensitivity, crystal and sequence data.
R J, Roberts, D, Macelis
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A method for identifying allele-specific hydroxymethylation
Epigenetics, 2020 We previously identified sequence-dependent allele-specific methylation (sd-ASM) in adult human peripheral blood leukocytes, in which ASM occurs in cis depending on adjacent polymorphic sequences.
Yoichi Yamada, Sho Sasaki
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Optimized In Vitro Restriction Digestion Protocol for Preparing Maize and Barley ddRAD-Seq Libraries
Agronomy, 2023 In recent years, high-throughput sequencing methods have become increasingly popular in molecular biology laboratories, mainly due to the relatively low cost of small, benchtop platforms, the simplicity of library preparation, and the low price per unit ...
Marta Puchta-Jasińska +3 more
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Sequence organization of feline leukemis virus DNA in infected cells [PDF]
, 1980 A restriction site map has been deduced of unintegrated and integrated FeLV viral DNA found in human RD cells after experimental infection with the Gardner-Arnstein strain of FeLV.
Casey, James W. +3 more
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Lack of class I H-2 antigens in cells transformed by radiation leukemia virus is associated with methylation and rearrangement of H-2 DNA [PDF]
, 1986 Transformation of murine thymocytes by radiation leukemia virus is associated with reduced expression of the class I antigens encoded in the major histocompatibility complex (MHC) and increased methylation and altered restriction enzyme patterns of MHC ...
Boral, Anthony +11 more
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Restricting SBH Ambiguity via Restriction Enzymes
Discrete Applied Mathematics, 2002 zbMATH Open Web Interface contents unavailable due to conflicting licenses.
Skiena, Steven, Snir, Sagi
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Differentiation between Aspergillus flavus and Aspergillus parasiticus from Pure Culture and Aflatoxin-Contaminated Grapes Using PCR-RFLP Analysis of aflR-aflJ Intergenic Spacer [PDF]
, 2011 Aflatoxins (AFs) represent the most important single mycotoxin-related food safety problem in developed and developing countries as they have adverse effects on human and animal health. They are produced mainly by Aspergillus flavus and A.
Atoui, Ali +5 more
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Nucleoside triphosphate-dependent restriction enzymes [PDF]
Nucleic Acids Research, 2001 The known nucleoside triphosphate-dependent restriction enzymes are hetero-oligomeric proteins that behave as molecular machines in response to their target sequences. They translocate DNA in a process dependent on the hydrolysis of a nucleoside triphosphate.
D. T. F. Dryden
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Restriction-Assembly: A Solution to Construct Novel Adenovirus Vector
Viruses, 2022 Gene therapy and vaccine development need more novel adenovirus vectors. Here, we attempt to provide strategies to construct adenovirus vectors based on restriction-assembly for researchers with little experience in this field.
Xiaojuan Guo +7 more
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