Results 41 to 50 of about 647,244 (359)
Brazilian Archives of Biology and Technology, 2008 The presence of restriction enzymes in the transformation mixture improved the efficiency of transformation in Moniliophthora perniciosa. The influence of the vector shape (linear or circular), the patterns of plasmid integration in genomic sites and the
Francis Julio Fagundes Lopes +4 more
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Restriction-Assembly: A Solution to Construct Novel Adenovirus Vector
Viruses, 2022 Gene therapy and vaccine development need more novel adenovirus vectors. Here, we attempt to provide strategies to construct adenovirus vectors based on restriction-assembly for researchers with little experience in this field.
Xiaojuan Guo +7 more
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Nucleoside triphosphate-dependent restriction enzymes [PDF]
Nucleic Acids Research, 2001 The known nucleoside triphosphate-dependent restriction enzymes are hetero-oligomeric proteins that behave as molecular machines in response to their target sequences. They translocate DNA in a process dependent on the hydrolysis of a nucleoside triphosphate.
Dryden, DTF, Murray, Noreen E, Rao, DN
openaire +4 more sources
"A PCR-RFLP Method to Identification of the Important Opportunistic Fungi: Candida Species, Cryptococcus neoformans, Aspergillus famigatus and Fusarium solani" [PDF]
Iranian Journal of Public Health, 2001 Deep-seated fungal infection present with non specific symptoms and involove a large number of different organisms. DNA-based technology offers for eariler detection of fungal pathogens and then earlier initiation of antifungal therapy.
SH Mirhendi +5 more
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BioTechniques, 2016 Directed evolution has greatly facilitated protein engineering and provided new insights into protein structure—function relationships. DNA shuffling using restriction enzymes is a particularly simple and cost-effective means of recombinatorial evolution
Weiliang Huang +3 more
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Julius-Kühn-Archiv, 2020 Hühnerhirse (Echinochloa crus-galli) gewinnt als Ungras im Maisanbau zunehmend an Bedeutung. Einseitiger Einsatz von ALS-Inhibitoren erzeugt einen Selektionsdruck, der zu vermehrtem Auftreten von Resistenzen führt.
Runge, Fabian +4 more
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Staphylococcus aureus typing by digestion of protein A coding gene using Bsp143I [PDF]
, 2014 Background: Protein A is the virulence factors of Staphylococcus aureus rolling in its pathogenesis, and its gene is used for typing. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with different enzymes has been used for ...
Ghaemi, E.A. +2 more
core +1 more source
DNA Cloning without Restriction Enzyme and Ligase
BioTechniques, 1999 One common problem in using the traditional DNA cloning procedure is that suitable natural restriction sites are often unavailable for a given task. Creating new restriction sites is often time consuming. Here, I describe a simple technique of producing “
Hung Tseng
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The Quasi-Steady-State Approximations revisited: Timescales, small parameters, singularities, and normal forms in enzyme kinetic [PDF]
Mathematical Biosciences, Volume 325, July 2020, 108339, 2019 In this work, we revisit the scaling analysis and commonly accepted conditions for the validity of the standard, reverse and total quasi-steady-state approximations through the lens of dimensional Tikhonov-Fenichel parameters and their respective critical manifolds.
arxiv +1 more source
A model of dynamic stability of H3K9me3 heterochromatin to explain the resistance to reprogramming of differentiated cells [PDF]
Biochim Biophys Acta. Gene Regulatory Mechanisms 2017
Feb;1860(2):184-195, 2017 Despite their dynamic nature, certain chromatin marks must be maintained over the long term. This is particulary true for histone 3 lysine 9 (H3K9) trimethylation, that is involved in the maintenance of healthy differentiated cellular states by preventing inappropriate gene expression, and has been recently identified as the most efficient barrier to ...
arxiv +1 more source