Results 41 to 50 of about 2,927,301 (234)
Julius-Kühn-Archiv, 2020 Hühnerhirse (Echinochloa crus-galli) gewinnt als Ungras im Maisanbau zunehmend an Bedeutung. Einseitiger Einsatz von ALS-Inhibitoren erzeugt einen Selektionsdruck, der zu vermehrtem Auftreten von Resistenzen führt.
Runge, Fabian +4 more
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Adapterama III: Quadruple-indexed, double/triple-enzyme RADseq libraries (2RAD/3RAD)
PeerJ, 2019 Molecular ecologists frequently use genome reduction strategies that rely upon restriction enzyme digestion of genomic DNA to sample consistent portions of the genome from many individuals (e.g., RADseq, GBS). However, researchers often find the existing
N. Bayona‐Vásquez +12 more
semanticscholar +1 more source
Nucleoside triphosphate-dependent restriction enzymes [PDF]
Nucleic Acids Research, 2001 The known nucleoside triphosphate-dependent restriction enzymes are hetero-oligomeric proteins that behave as molecular machines in response to their target sequences. They translocate DNA in a process dependent on the hydrolysis of a nucleoside triphosphate.
Dryden, DTF, Murray, Noreen E, Rao, DN
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PLoS ONE, 2012 Advancements in next-generation sequencing technology have enabled whole genome re-sequencing in many species providing unprecedented discovery and characterization of molecular polymorphisms. There are limitations, however, to next-generation sequencing
J. Poland +3 more
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DNA Cloning without Restriction Enzyme and Ligase
BioTechniques, 1999 One common problem in using the traditional DNA cloning procedure is that suitable natural restriction sites are often unavailable for a given task. Creating new restriction sites is often time consuming. Here, I describe a simple technique of producing “
Hung Tseng
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Nucleic Acids Research, 2009 Atomic force microscopy (AFM) allows the study of single protein–DNA interactions such as those observed with the Type I Restriction–Modification systems.
Kelly J. Neaves +6 more
semanticscholar +1 more source
Identifikasi polimorfisme gen GH (Growth Hormone) sapi Bali dengan metode PCR-RFLP
Journal of Biological Researches, 2012 This study was conducted to identify polymorphism of growth hormone gene of Bali cattle. A PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) procedure was developed for determining polymorphism of growth hormone gene.
Sutiman Bambang Sumitro +3 more
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APOBEC3-mediated restriction of RNA virus replication
Scientific Reports, 2018 APOBEC3 family members are cytidine deaminases with roles in intrinsic responses to infection by retroviruses and retrotransposons, and in the control of other DNA viruses, such as herpesviruses, parvoviruses and hepatitis B virus.
A. Milewska +7 more
semanticscholar +1 more source
Restriction & Modification Enzymes
, 2022 Restriction and modification enzymes serve as nature’s genetic gatekeepers. These specialized proteins function together as a defence mechanism in bacteria and protect them from invading foreign DNA, such as that of bacteriophages. Restriction enzymes, also known as restriction endonucleases, recognize specific sequences within the DNA and cleave it at
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Restriction enzyme digestion of hemimethylated DNA [PDF]
Nucleic Acids Research, 1981 Hemimethylated duplex DNA of the bacteriophage phi X 174 was synthesized using primed repair synthesis is in vitro with E. coli DNA polymerase I followed by ligation to produce the covalently closed circular duplex (RFI). Single-stranded phi X DNA was used as a template, a synthetic oligonucleotide as primer and 5-methyldeoxycytidine-5'-triphosphate ...
Howard Cedar +2 more
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