Results 91 to 100 of about 3,087,992 (329)

Time-resolved fluorescence studies of nucleotide flipping by restriction enzymes

Nucleic Acids Research, 2009
Restriction enzymes Ecl18kI, PspGI and EcoRII-C, specific for interrupted 5-bp target sequences, flip the central base pair of these sequences into their protein pockets to facilitate sequence recognition and adjust the DNA cleavage pattern. We have used
R. Neely, G. Tamulaitis, Kai Chen, M. Kubala, V. Šikšnys, Anita C Jones   +5 more
semanticscholar   +1 more source

Ergothioneine supplementation improves pup phenotype and survival in a murine model of spinal muscular atrophy

FEBS Letters, EarlyView.
Spinal muscular atrophy (SMA) is a genetic disease affecting motor neurons. Individuals with SMA experience mitochondrial dysfunction and oxidative stress. The aim of the study was to investigate the effect of an antioxidant and neuroprotective substance, ergothioneine (ERGO), on an SMNΔ7 mouse model of SMA.
Francesca Cadile, Daniela Ratto, Giorgia Rastelli, Ottavia Eleonora Ferraro, Caterina Temporini, Sunil Kumar, Simona Boncompagni, Paola Rossi, Monica Canepari   +8 more
wiley   +1 more source

RFLP-kenzy: a new bioinformatics tool for in silico detection of key restriction enzyme in RFLP technique

Beni-Suef University Journal of Basic and Applied Sciences
Background Today, several bioinformatics tools are available for analyzing restriction fragment length data. RFLP-kenzy is a new bioinformatic tool for identifying restriction key enzyme that cut at least 1 sequence and a maximum of n-1 sequence. Results
Nora Laref, Khadidja Belkheir, Mohamed Belazreg, Abdelhadi Hireche   +3 more
doaj   +1 more source

A Stable Genetic Transformation System and Implications of the Type IV Restriction System in the Nitrogen-Fixing Plant Endosymbiont Frankia alni ACN14a

Frontiers in Microbiology, 2019
Genus Frankia is comprised primarily of nitrogen-fixing actinobacteria that form root nodule symbioses with a group of hosts known as the actinorhizal plants.
Isaac Gifford, Summer Vance, Giang Nguyen, Alison M. Berry   +3 more
doaj   +1 more source

In vitro Type II Restriction of Bacteriophage DNA With Modified Pyrimidines

Frontiers in Microbiology, 2020
To counteract host-encoded restriction systems, bacteriophages (phages) incorporate modified bases in their genomes. For example, phages carry in their genomes modified pyrimidines such as 5-hydroxymethyl-cytosine (5hmC) in T4gt deficient in α- and β ...
Kiersten Flodman, Ivan R. Corrêa, Nan Dai, Peter Weigele, Shuang-yong Xu   +4 more
doaj   +1 more source

OTU deubiquitinases reveal mechanisms of linkage specificity and enable ubiquitin chain restriction analysis [PDF]

, 2013
Sixteen ovarian tumor (OTU) family deubiquitinases (DUBs) exist in humans, and most members regulate cell-signaling cascades. Several OTU DUBs were reported to be ubiquitin (Ub) chain linkage specific, but comprehensive analyses are missing, and the ...
Akutsu, Masato, Arnaudo, Nadia, Ekkebus, Reggy, El Oualid, Farid, Elliott, Paul R., Freund, Stefan M. V., Geurink, Paul P., Hospenthal, Manuela K., Komander, David, Kulathu, Yogesh, Mevissen, Tycho E. T., Ovaa, Huib, Wauer, Tobias   +12 more
core  

Expression of functional recombinant human tissue transglutaminase (TG2) using the bac-to-bac baculovirus expression system [PDF]

, 2016
Purpose: Tissue transglutaminase (TG2) is a unique multifunctional enzyme. The enzyme possesses enzymatic activities such as transamidation/crosslinking and non-enzymatic functions such as cell migration and signal transduction.
Azari, S., Kalhor, H.R., Yazdani, Y.
core   +2 more sources

Microbial exopolysaccharide production by polyextremophiles in the adaptation to multiple extremes

FEBS Letters, EarlyView.
Polyextremophiles are microorganisms that endure multiple extreme conditions by various adaptation strategies that also include the production of exopolysaccharides (EPSs). This review provides an integrated perspective on EPS biosynthesis, function, and regulation in these organisms, emphasizing their critical role in survival and highlighting their ...
Tracey M Gloster, Ebru Toksoy Öner
wiley   +1 more source

Sequence-specific double-strand cleavage of DNA by penta-N-methylpyrrolecarboxamide-EDTA·Fe(II) [PDF]

, 1983
In the presence of O2 and 5 mM dithiothreitol, penta-N-methylpyrrolecarboxamide-EDTA·Fe(II) [P5E·Fe(II)] at 0.5 µ M cleaves pBR322 plasmid DNA (50 µ M in base pairs) on opposite strands to afford discrete DNA fragments as analyzed by agarose gel ...
Dervan, Peter B., Schultz, Peter G.
core  

Poly(ADP-ribosyl)ation is involved in the epigenetic control of TET1 gene transcription [PDF]

, 2014
TET enzymes are the epigenetic factors involved in the formation of the Sixth DNA base 5-hydroxymethylcytosine, whose deregulation has been associated with tumorigenesis.
Bacalini, MARIA GIULIA, Caiafa, Paola, Calabrese, Roberta, Ciccarone, Fabio, Franceschi, C, Guastafierro, Tiziana, Mariano, Germano, Reale, Anna, Valentini, Elisabetta, Zampieri, Michele   +9 more
core   +5 more sources