Typing of Campylobacter jejuni isolated from poultry on the basis of flaA-RFLP by various restriction enzymes. [PDF]
Vet Anim Sci, 2018 Yadav R +3 more
europepmc +1 more source
A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms
FEBS Open Bio, EarlyView.We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley +1 more source
Endonuclease specificity and sequence dependence of type IIS restriction enzymes. [PDF]
PLoS One, 2015 Lundin S +8 more
europepmc +1 more source
Nanobody-mediated modulation of long RSH enzymes Rel and RelA catalysis by restriction of their conformational landscape [PDF]
Katleen Van Nerom +14 more
openalex +1 more source
Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
FEBS Open Bio, EarlyView.This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
FEBS Open Bio, EarlyView.We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source
Expanding the scope of methylation-sensitive restriction enzyme (MSRE) PCR for forensic identification of body fluids through the novel use of methylation-dependent restriction enzymes (MDRE) and the combination of autosomal and Y-chromosomal markers. [PDF]
Int J Legal MedRothe J +6 more
europepmc +1 more source
Type I restriction enzymes and their relatives. [PDF]
Nucleic Acids Res, 2014 Loenen WA +3 more
europepmc +1 more source
Site-specific DNA transesterification catalyzed by a restriction enzyme
, 2007 Giedrius Sasnauskas +3 more
openalex +1 more source