Results 191 to 200 of about 3,087,992 (329)

Current trends in single‐cell RNA sequencing applications in diabetes mellitus

FEBS Open Bio, EarlyView.
Single‐cell RNA sequencing is a powerful approach to decipher the cellular and molecular landscape at a single‐cell resolution. The rapid development of this technology has led to a wide range of applications, including the detection of cellular and molecular mechanisms and the identification and introduction of novel potential diagnostic and ...
Seyed Sajjad Zadian, Khodakaram Jahanbin, Shekoofeh Nikooei, Marzieh Rostaminejad, Arezoo Rahimi, Pedram Abdizadeh, Behnam Alipoor   +6 more
wiley   +1 more source

Pyrite cloning: a single tube and programmed reaction cloning with restriction enzymes. [PDF]

Plant Methods, 2018
Fischer MD, Mgboji E, Liu Z.
europepmc   +1 more source

An optimized protocol to detect high‐throughput DNA methylation from custom targeted sequences on 96 samples simultaneously

FEBS Open Bio, EarlyView.
Workflow of a high‐throughput technology for epigenotyping of differentially methylated CpGs in specific regions of the genome. The protocol works with small amounts of DNA extracted from blood or semen. The protocol consists of both enzymatic conversion of unmethylated cytosines and capture by hybridization with a custom panel.
Nathalie Iannuccelli, Sophie Valière, Julien Sarry, Cécile Donnadieu, Julie Demars   +4 more
wiley   +1 more source

Diffdigester.uni-jena.de: a tool for optimized selection of restriction enzymes for plasmid identification in cloning procedures. [PDF]

Nucleic Acids Res
Gühmann M, Reuter S, Mrowka R.
europepmc   +1 more source

Further Probing of Cu²⁺-Dependent PNAzymes Acting as Artificial RNA Restriction Enzymes. [PDF]

Molecules, 2019
Luige O, Murtola M, Ghidini A, Strömberg R.   +3 more
europepmc   +1 more source

Phosphomimetic mutations near active sites of proteins in Thermus thermophilus suggest a widespread regulatory mechanism

FEBS Open Bio, EarlyView.
In Thermus thermophilus, more than half of the phosphorylation sites identified by proteomic analysis are located near the active site. All phosphomimetic mutants of phosphosites of six enzymes belonging to different families showed severely reduced activity compared with the wild‐type, particularly in the turnover number.
Anzu Nishiwaki, Hiroki Okanishi, Yoshikatsu Kanai, Ryoji Masui   +3 more
wiley   +1 more source

Using nucleolytic toxins as restriction enzymes enables new RNA applications. [PDF]

Nucleic Acids Res
Rothweiler U, Gundesø SE, Mikalsen EW, Svenning S, Singh M, Combes F, Pettersson FJ, Mangold A, Piotrowski Y, Schwab F, Lanes O, Striberny BK.   +11 more
europepmc   +1 more source

Restriction Enzymes as a Target for DNA-Based Sensing and Structural Rearrangement. [PDF]

ACS Omega, 2018
Buckhout-White S, Person C, Medintz IL, Goldman ER.   +3 more
europepmc   +1 more source

Statins induce monocytic differentiation in acute myeloid leukemia cells through the KLF4/DPYSL2A axis

FEBS Open Bio, EarlyView.
Statins, identified via the Comparative Toxicogenomics Database, promote monocytic differentiation and apoptosis in non‐APL AML cells by upregulating DPYSL2A through a KLF4‐dependent pathway. Mevalonate supplementation reversed these effects, indicating involvement of the mevalonate pathway.
Mina Noura, Kota Shoji, Michidai Nobe, Moe Ishikawa, Miu Tanaka, Akiko Okayama, Souichi Adachi, Hidemasa Matsuo   +7 more
wiley   +1 more source

An enterococcal phage protein inhibits type IV restriction enzymes involved in antiphage defense. [PDF]

Nat Commun
Bullen NP, Johnson CN, Andersen SE, Arya G, Marotta SR, Lee YJ, Weigele PR, Whitney JC, Duerkop BA.   +8 more
europepmc   +1 more source