Results 231 to 240 of about 3,087,992 (329)

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Requirements for double-strand cleavage by chimeric restriction enzymes with zinc finger DNA-recognition domains.

Nucleic Acids Research, 2000
Jeffrey S. Smith, Marina Bibikova, F. Whitby, Attipalli Ramachandra Reddy, Attipalli Ramachandra Reddy, S. Chandrasegaran, Dana Carroll   +6 more
semanticscholar   +1 more source

Comparison of restriction enzymes for pulsed-field gel electrophoresis typing of Moraxella catarrhalis. [PDF]

J Clin Microbiol, 2013
Marti S, Puig C, Domenech A, Liñares J, Ardanuy C.   +4 more
europepmc   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

Evaluation of a Quantitative DNA Methylation Analysis Technique using Methylation-Sensitive/Dependent Restriction Enzymes and Real-Time PCR

Epigenetics, 2006
C. Oakes, S. La Salle, B. Robaire, J. Trasler   +3 more
semanticscholar   +1 more source

Improving electrochemical hybridization assays with restriction enzymes.

Chem Commun (Camb)
Zhou X, Zamani M, Austin K, De Bock M, Chaj Ullola J, Riki S, Furst AL.   +6 more
europepmc   +1 more source

Revised selection criteria for candidate restriction enzymes in genome walking. [PDF]

PLoS One, 2012
Taheri A, Robinson SJ, Parkin I, Gruber MY.   +3 more
europepmc   +1 more source

Molecular determinants of signal transduction in tropomyosin receptor kinases

FEBS Open Bio, EarlyView.
Tropomyosin receptor kinases control critical neuronal functions, but how do the same receptors produce diverse cellular responses? This review explores the structural mechanisms behind Trk signaling diversity, focusing on allosteric modulation and ligand bias.
Giray Enkavi
wiley   +1 more source

ATG4B is required for mTORC1‐mediated anabolic activity and is associated with clinical outcomes in non‐small cell lung cancer

FEBS Open Bio, EarlyView.
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan, Bethany C. Guerra, Selina Uranga, Jessica M. Cardin, Steven E. Riechman, Mariana Janini Gomes, James D. Fluckey   +6 more
wiley   +1 more source