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Restriction Enzymes

Restriction enzymes are bacterial enzymes that cleave DNA at specific recognition sequences, usually consisting of four to eight base pairs. These enzymes have become invaluable tools in molecular biology, enabling scientists to manipulate and analyze DNA in various ways. Restriction enzymes are used in various applications, including gene cloning, DNA
Srinivasan Chandrasegaran, Karthikeyan Kandavelou   +1 more
exaly   +5 more sources

ATP-dependent restriction enzymes

, 2000
The phenomenon of restriction and modification (R-M) was first observed in the course of studies on bacteriophages in the early 1950s. It was only in the 1960s that work of Arber and colleagues provided a molecular explanation for the host specificity.
Rao, Desirazu N, Saha, Swati, Krishnamurthy, Vinita   +2 more
openaire   +3 more sources

S-Adenosyl-L-methionine–Dependent Restriction Enzymes

Critical Reviews in Biochemistry and Molecular Biology, 2004
Restriction-modification (R-M) enzymes are classified into type I, II, III, and IV, based on their recognition sequence, subunit composition, cleavage position, and cofactor requirements.
Desirazu N Rao
exaly   +1 more source

Programmable DNA-Guided Artificial Restriction Enzymes

ACS Synthetic Biology, 2017
Restriction enzymes are essential tools for recombinant DNA technology that have revolutionized modern biological research. However, they have limited sequence specificity and availability.
Huimin Zhao, Behnam Enghiad
exaly   +2 more sources

Restriction Enzyme Computation

2003
In this paper implementation of an inner hair cell model, including macromechanics and mechanical to neural transduction process is presented and discussed. The well-known Meddis model will be use as the reference system, and a high level synthesis will provide a parametrizable implementation and a reusability code, which allows future refinements on ...
Olgierd Unold, Maciej Troc
openaire   +1 more source

Restriction enzymes in cells, not eppendorfs

Trends in Microbiology, 1994
Restriction enzymes are essential reagents to molecular biologists, but their relevance to bacterial populations is less obvious. Most bacteria encode restriction and modification systems and these are commonly considered to be a barrier to phage infection. Current evidence also supports a more general role for them in genetic recombination.
G, King, N E, Murray
openaire   +2 more sources

Recognition Sequence of a Restriction Enzyme

Nature New Biology, 1973
Restriction endonuclease EcoRII makes about twenty double-stranded breaks per molecule of λh80 DNA. The 5′-terminal sequences are pC-C-A-G-G and pC-C-T-G-G. These are complementary and rotationally symmetrical, showing how the enzyme may produce DNA fragments with short cohesive ends.
C H, Bigger, K, Murray, N E, Murray
openaire   +2 more sources

Class-IIS restriction enzymes — a review

Gene, 1991
Class-IIS restriction enzymes (ENases-IIS) interact with two discrete sites on double-stranded DNA: the recognition site, which is 4-7 bp long, and the cleavage site, usually 1-20 bp away from the recognition site. The recognition sequences of ENases-IIS are totally (or partially) asymmetric and all of the characterized ENases-IIS are monomeric.
Szybalski, W, Kim, SC Kim, Sun-Chang, Hasan, N, Podhajska, AJ   +3 more
openaire   +2 more sources