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Purification and Culture of Retinal Ganglion Cells

Cold Spring Harbor Protocols, 2013
Retinal ganglion cells (RGCs) are the neurons that extend axons through the optic nerve, connecting and transmitting information from the retina to the brain. In mammals, RGCs receive information from bipolar and amacrine cells and synapse onto target cells in the lateral geniculate nucleus (LGN) as well as the superior colliculus.
Alissa Winzeler, Jack T. Wang
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Retinal Ganglion Cells

1988
Ganglion cells typically make up the innermost layer of the vertebrate retina. They are the only cells with axons that leave the eye. These axons form the optic nerve. Ganglion cell dendrites ramify in the inner nuclear layer, where they are postsynaptic to bipolar cells, which provide a direct input pathway from the outer plexiform layer, and to ...
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Culture of rat retinal ganglion cells

Journal of Huazhong University of Science and Technology [Medical Sciences], 2011
This study aimed to modify the mixed and purified culture of rat retinal ganglion cells (RGCs) in vitro. The retinae of 1-3 day old Sprague-Dawley (SD) rats were separated bluntly into two layers: inner layer and outer layer, under a surgical microscope. Retinal cells isolated from different layers (inner layer, outer layer and whole retinal tissue) by
Fei Chen   +4 more
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Concerted Signaling by Retinal Ganglion Cells [PDF]

open access: possibleScience, 1995
To analyze the rules that govern communication between eye and brain, visual responses were recorded from an intact salamander retina. Parallel observation of many retinal ganglion cells with a microelectrode array showed that nearby neurons often fired synchronously, with spike delays of less than 10 milliseconds.
Markus Meister   +2 more
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Studies on the Retinal Ganglion Cells

1961
In recent studies (6, 15), it has definitively been proved, by histological localization of the recording electrode tip, that the S-potential has an intracellular origin in the tangential and radial glial systems of the retina ; the L-response was obtained in the horizontal cells and the C-response in the Muller fibers.
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The Retinal Ganglion Cell Layer.

Archives of Ophthalmology, 1965
This book describes a series of careful and detailed histologic studies of the retina in which the topographical arrangements of the ganglion cells were related to lesions at various levels of the visual pathways in the central nervous system. The experimental material from macaques and tissue from clinical human cases were stained by conventional ...
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Classification of turtle retinal ganglion cells

Journal of Neurophysiology, 1989
1. Receptive fields of 78 retinal ganglion cells were analyzed for their responses to moving and stationary lights that were presented under a variety of stimulus conditions. All cells were sensitive to moving stimuli, and their receptive fields often comprised excitatory and inhibitory sub-regions. 2.
J. E. Fulbrook, A. M. Granda
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Towards Retinal Ganglion Cell Regeneration

Regenerative Medicine, 2012
Traumatic optic nerve injury and glaucoma are among the leading causes of incurable vision loss across the world. What is worse, neither pharmacological nor surgical interventions are significantly effective in reversing or halting the progression of vision loss.
Qisheng Tang   +7 more
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Topography of pig retinal ganglion cells

Journal of Comparative Neurology, 2005
AbstractIn the present work we analyzed the distribution of retinal ganglion cells (RGCs) in the pig retina. RGCs were retrogradely labeled in vivo by injecting Fluoro‐Gold into the optic nerve. RGC density and the distribution of RGCs in terms of soma size were analyzed. Different regions of the porcine retina were identified following analysis of the
Javier Ruiz-Ederra   +3 more
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Peptides influence retinal ganglion cells

Neuroscience Letters, 1981
Neurotensin, substance P and (D-Ala2, Met5)-enkephalinamide were iontophoresed onto ganglion cells of the amphibian retina. Substance P and neurotensin were found to be excitatory while (D-Ala2, Met5)-enkephalinamide suppressed all types of ganglion cells. These findings are consistent with a functional role for peptides in the vertebrate retina.
Evan Dick, Robert F. Miller
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