Results 171 to 180 of about 335,945 (253)

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

A rapid yeast-based reverse genetics system reveals SARS-CoV-2 Omicron BA.2.86 variant spreads faster than Omicron JN.1 variant in primary human nasal and bronchial epithelial airway cultures. [PDF]

mBio
Xu J, Chamblee M, Hsu CC, Jiang F, Chen P, Zhang Y, Liang X, Amer AO, Boyaka PN, Cormet-Boyaka E, Peeples ME, Li J.   +11 more
europepmc   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

How phagocytic cells kill bacteria: Lessons from a professional killer

FEBS Open Bio, EarlyView.
How phagocytic cells ingest and kill bacteria has been studied for more than a century, but many questions remain unanswered. The study of the amoeba Dictyostelium discoideum brings new answers, and new questions. Professional phagocytic cells such as neutrophils and macrophages, as well as free‐living soil amoebae like Dictyostelium discoideum, employ
Otmane Lamrabet, Pierre Cosson
wiley   +1 more source

Correction: Development and characterization of reverse genetics systems of feline infectious peritonitis virus for antiviral research. [PDF]

Vet Res
Gu G, Fung TS, Hung WT, Osterrieder N, Go YY.   +4 more
europepmc   +1 more source

Reverse genetics

Behavioural Pharmacology, 2007
Homberg, J. R., Mul, J. D., Oliviere, J. J. D., De Boer, S. F., Cools, A. R., Schoffelmeer, A. N. M., Vanderschuren, L. J. M. J., De Vries, T. J., Ellenbroek, B. A., Cuppen, E.   +9 more
openaire   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Rescue of bovine ephemeral fever virus through reverse genetics, but inability to propagate. [PDF]

Virusdisease
Jasmeen P, Gupta P, Kaur C, Gauthami S, Pyasi S, Nayak D, Hegde NR.   +6 more
europepmc   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

Plasmid-Based Reverse Genetics System Enabling One-Step Generation of Genotype 3 Hepatitis E Virus. [PDF]

Viruses
Kobayashi T, Nishiyama T, Yamada K, Murata K, Okamoto H.   +4 more
europepmc   +1 more source