Results 171 to 180 of about 1,866 (214)
Some of the next articles are maybe not open access.
Reverse Genetics Systems for Filoviruses
2023Filoviruses are causative agents of severe hemorrhagic fevers with high case fatality rates in humans. For studies of virus biology and the subsequent development of countermeasures, reverse genetic systems, and especially those facilitating the generation of recombinant filoviruses, are indispensable.
Bianca S, Bodmer, Thomas, Hoenen
openaire +2 more sources
Reverse Genetic Approaches in Zebrafish
Journal of Genetics and Genomics, 2012Zebrafish (Danio rerio) is a well-established vertebrate animal model. A comprehensive collection of reverse genetics tools has been developed for studying gene function in this useful organism. Morpholino is the most widely used reagent to knock down target gene expression post-transcriptionally.
Peng, Huang +3 more
openaire +2 more sources
Reverse Genetics of Influenza Viruses
2012The ability to modify influenza viruses at will has revolutionized influenza research. Reverse genetics has been used to generate mutant or reassortant influenza viruses to assess their replication, virulence, pathogenicity, host range, and transmissibility.
Gabriele, Neumann +2 more
openaire +2 more sources
Reverse Genetics for Mammalian Orthoreovirus
2017Reverse genetics allows introduction of specific alterations into a viral genome. Studies performed with mutant viruses generated using reverse genetics approaches have contributed immeasurably to our understanding of viral replication and pathogenesis, and also have led to development of novel vaccines and virus-based vectors.
Johnasha D, Stuart +2 more
openaire +2 more sources
Prions: Pathogenesis and Reverse Genetics
Annals of the New York Academy of Sciences, 2000Abstract: Spongiform encephalopathies are a group of infectious neurodegenerative diseases. The infectious agent that causes transmissible spongiform encephalopathies was termed prion by Stanley Prusiner. The prion hypothesis states that the partially protease‐resistant and detergent‐insoluble prion protein (PrPsc) is identical with the infectious ...
Aguzzi, A +8 more
openaire +4 more sources
Genetics and Reverse Genetics of Nidoviruses
2014The genomes of nidoviruses are infectious, and virus replication is initiated as the genome is delivered to the cytoplasm and the replicase is translated by the host cell ribosomes. Nidovirus reverse-genetics systems are needed to better understand aspects of their complex replication strategy, pathogenesis, and mechanisms of host range expansion, and ...
Damon J. Deming, Ralph S. Baric
openaire +1 more source
2020
This chapter focuses on reverse genetics in which the start point is the DNA sequence of a cloned gene, and the mutant phenotype—and hence biological function of the gene—is inferred from that sequence. The chapter starts with an exploration of the reverse genetic analysis carried out on budding yeast and mice.
openaire +1 more source
This chapter focuses on reverse genetics in which the start point is the DNA sequence of a cloned gene, and the mutant phenotype—and hence biological function of the gene—is inferred from that sequence. The chapter starts with an exploration of the reverse genetic analysis carried out on budding yeast and mice.
openaire +1 more source
Reverse Genetics and Cystic Fibrosis
American Journal of Respiratory Cell and Molecular Biology, 1990Abstract The protein responsible for cystic fibrosis has been identified using an approach called “reverse” genetics. This approach relies on the chromosomal map position to direct the search for a disease gene, several novel cloning strategies to isolate the gene, and the gene's sequence to define the abnormal protein.
M C, Iannuzzi, F S, Collins
openaire +2 more sources
1999
Abstract The nematode Caenorhabditis elegans has about 19000 genes. Approximately 2000 of these have been identified in forward genetic screens for animals with mutant phenotypes. The generation and analysis of such mutants has been the key to the success of the C. elegans model because the in vivo function of a gene can be inferred from
openaire +1 more source
Abstract The nematode Caenorhabditis elegans has about 19000 genes. Approximately 2000 of these have been identified in forward genetic screens for animals with mutant phenotypes. The generation and analysis of such mutants has been the key to the success of the C. elegans model because the in vivo function of a gene can be inferred from
openaire +1 more source
Reverse Genetics of Zika Virus
2017We have established a reverse genetic system for Zika virus (ZIKV). Five shuttle plasmids were constructed and assembled into the full-length cDNA clone of ZIKV genome. To ensure the stability of the cDNA clone, we used a low copy vector (pACYC177) and a set of unique restriction enzyme sites on the ZIKV genome to assemble the full-length cDNA clone. A
Chao, Shan, Xuping, Xie, Pei-Yong, Shi
openaire +2 more sources