Research Progress and Applications of the Rotavirus Reverse Genetics System [PDF]
AnimalsRotavirus is a leading cause of severe, dehydrating diarrhea in infants and young animals, causing significant global morbidity and mortality. For decades, research was hindered by challenges in establishing reverse genetics systems due to the virus’s ...
Yiqun Chen +9 more
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Application of versatile reverse genetics system for feline coronavirus [PDF]
Microbiology SpectrumFeline infectious peritonitis (FIP) is a fatal disease caused by feline coronavirus (FCoV). Although multiple gene mutations in FCoV likely account for FIP pathogenesis, molecular studies for FCoV have been limited due to the lack of a suitable reverse ...
Izumi Kida +5 more
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Re-Examining Rotavirus Innate Immune Evasion: Potential Applications of the Reverse Genetics System [PDF]
mBio, 2022 Rotaviruses represent one of the most successful pathogens in the world, with high infectivity and efficient transmission between the young of many animal species, including humans.
Avan Antia +2 more
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A mix-and-match reverse genetics system for evaluating genetic determinants of orthobunyavirus neurological disease. [PDF]
PLoS ONEThe encephalitic orthobunyaviruses have tri-segmented, negative sense RNA genomes and can cause severe neurological disease in humans, including La Crosse virus (LACV), which is the leading cause of pediatric arboviral encephalitis in the United States ...
Heini M Miettinen +2 more
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One-pot Golden Gate Assembly of an avian infectious bronchitis virus reverse genetics system. [PDF]
PLoS ONEAvian infectious bronchitis is an acute respiratory disease of poultry of particular concern for global food security. Investigation of infectious bronchitis virus (IBV), the causative agent of avian infectious bronchitis, via reverse genetics enables ...
Katharina Bilotti +13 more
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Establishment of a reverse genetics system for an epidemic strain of porcine rotavirus JXAY01 type G5P[23]I12 [PDF]
Frontiers in Veterinary SciencePorcine rotavirus is one of the most important pathogens causing diarrhea in newborn piglets, and the genome of this virus contains 11 double-stranded RNA segments, which are easy to be recombined among strains to produce new strains with different ...
Changjin Liu +8 more
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Reverse Genetics System for Rabbit vesivirus [PDF]
Frontiers in Microbiology, 2020 Most caliciviruses are refractory to replication in cell culture and only a few members of the family propagate in vitro. Rabbit vesivirus (RaV) is unique due to its ability to grow to high titers in several animal and human cell lines.
Ángel L. Álvarez +8 more
doaj +2 more sources
Development of a New Reverse Genetics System for Ebola Virus [PDF]
mSphere, 2021 Ebola virus is among the most dangerous viral pathogens, with a case fatality rate of up to 90%. Since 2013, the two largest and most complex Ebola outbreaks in West Africa have revealed the lack of investigation on this notorious virus.
Tianyu Gan +8 more
doaj +2 more sources
Identification of an optimal exogenous gene insertion site (P-M) and establishment of a reverse genetics system for aMPV/A. [PDF]
PLoS ONEAvian metapneumovirus subgroup A (aMPV/A) causes widespread infections in commercial poultry worldwide. The development of highly efficient viral vector vaccines is constrained by multiple factors, among which the lack of a mature reverse genetics system
Yu Guo +9 more
doaj +2 more sources
Development of a reverse genetics system for West Nile virus (Kunjin type) [PDF]
Frontiers in Veterinary ScienceKunjin virus (KUNV), a naturally attenuated strain of West Nile virus (WNV), shares similar transmission modes and hosts—primarily mosquitoes, birds, and horses.
Zhen Wu +60 more
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