Results 21 to 30 of about 18,427,402 (231)

One-Step Assembly of a Porcine Epidemic Diarrhea Virus Infectious cDNA Clone by Homologous Recombination in Yeast: Rapid Manipulation of Viral Genome With CRISPR/Cas9 Gene-Editing Technology

Frontiers in Microbiology, 2022
Porcine epidemic diarrhea virus (PEDV), a swine enteric coronavirus causing acute diarrhea in piglets, is one of the major threatens to the pork industry globally.
Yanyang Zhou, Chenxi Li, Chenxi Li, Chenxi Li, Cicheng Ren, Jingbo Hu, Changxu Song, Xinjie Wang, Yanhua Li, Yanhua Li, Yanhua Li   +10 more
doaj   +1 more source

Establishment of a reverse genetics system for Schmallenberg virus, a newly emerged orthobunyavirus in Europe [PDF]

, 2013
Schmallenberg virus (SBV) is a newly emerged orthobunyavirus that has caused widespread disease in cattle, sheep and goats in Europe. Like other orthobunyaviruses, SBV is characterized by a tripartite negative-sense RNA genome that encodes four ...
Szemiel, A.M., McLees, A., Li, P., van Knippenberg, I.C., McLees, Angela, Li, Ping, Shi, X., van Knippenberg, Ingeborg C., Elliott, Richard M., Szemiel, Agnieszka M., Shi, Xiaohong, Koudriakova, E., Koudriakova, Elina, Blakqori, G., Elliott, R.M., Blakqori, Gjon   +15 more
core   +1 more source

A plasmid DNA-launched SARS-CoV-2 reverse genetics system and coronavirus toolkit for COVID-19 research

, 2021
The recent emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the underlying cause of Coronavirus Disease 2019 (COVID-19), has led to a worldwide pandemic causing substantial morbidity, mortality, and economic devastation.
Brennan, Benjamin, Liu, Xiang, Turnbull, Matthew L, Alexander, Akira JT, Zaid, Ali, Mahalingam, Suresh, Brunker, Kirstyn, Merits, Andres, Brown, Fiona, Baillie, Carla, Bakshi, Siddharth, Rihn, Suzannah J, et al., Wickenhagen, Arthur, Freitas, Joseph R   +14 more
core   +1 more source

Establishment of a Plasmid-Based Reverse Genetics System for the Cell Culture-Adapted Hepatitis E Virus Genotype 3c Strain 47832c

Pathogens, 2020
The hepatitis E virus (HEV) causes acute and chronic hepatitis in humans. Investigation of HEV replication is hampered by the lack of broadly applicable, efficient cell culture systems and tools for site-directed mutagenesis of HEV.
Johannes Scholz, Christine Bächlein, Ashish K. Gadicherla, Alexander Falkenhagen, Simon H. Tausch, Reimar Johne   +5 more
doaj   +1 more source

Identification of Virulence Associated Region during Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus during Attenuation In Vitro: Complex Question with Different Strain Backgrounds

Viruses, 2021
Highly pathogenic porcine reproductive and respiratory syndrome virus PRRSV (HP-PRRSV) was one of the most devastating diseases of the pig industry, among various strategies, vaccination was one of the most useful tools for PRRS control.
Yifeng Jiang, Wu Tong, Lingxue Yu, Liwei Li, Fei Gao, Guoxin Li, Changlong Liu, Pengfei Chen, Qi Shen, Yujiao Zhang, Yanjun Zhou, Guangzhi Tong   +11 more
doaj   +1 more source

A Reverse Genetics System for dsRNA Viruses [PDF]

, 2007
SummaryThe advent of a new reverse genetics system for reoviruses, described in this issue of Cell Host & Microbe by Kobayashi et al., opens new horizons of investigation into the molecular mechanisms governing dsRNA virus replication and pathogenesis ...
Baric, Ralph S., Sims, Amy C.
core   +1 more source

Dual Promoters Improve the Rescue of Recombinant Measles Virus in Human Cells

Viruses, 2021
Reverse genetics is a technology that allows the production of a virus from its complementary DNA (cDNA). It is a powerful tool for analyzing viral genes, the development of novel vaccines, and gene delivery vectors.
Soroth Chey, Juliane Maria Palmer, Laura Doerr, Uwe Gerd Liebert   +3 more
doaj   +1 more source

A Recombinant Porcine Reproductive and Respiratory Syndrome Virus Stably Expressing a Gaussia Luciferase for Antiviral Drug Screening Assay and Luciferase-Based Neutralization Assay

Frontiers in Microbiology, 2022
The reverse genetics system is a valuable tool in the virological study of RNA viruses. With the availability of reverse genetics, the porcine reproductive and respiratory syndrome virus (PRRSV) has been utilized as a viral vector for the expression of ...
Yanhua Li, Yanhua Li, Yanhua Li, Cicheng Ren, Chenxi Li, Chenxi Li, Chenxi Li, Yihong Xiao, Yanyang Zhou   +8 more
doaj   +1 more source

Community genetics. Its definition 2010 [PDF]

, 2010
This paper presents a definition of the medical field of community genetics. It starts with a brief historical overview, defines the requirements for an adequate definition, presents the definition, and discusses the constituent parts of the ...
Kate, L.P. ten, Hamamy, H., Arnold Christianson, Ulf Kristoffersson, Lihadh Al-Gazali, Rahman, P., Jean-Jacques Cassiman, Marais, A.D., Kate, L.P., Proton Rahman, Kristoffersson, U., Victor B. Penchaszadeh, Bittles, A., Cornel, M.C., ten Kate, L.P., Kääriäinen, H., Cassiman, J.J., Christianson, A., Hanan Hamamy, Al-Gazali, L., Schmidtke, J., David Marais, Leo P. ten Kate, Jörg Schmidtke, Helena Kääriäinen, Martina C. Cornel, Anand, S., Penchaszadeh, V.B., Alan Bittles, Sonia Anand, Marais, D., Cassiman, J-J, Leo P. ten Kate   +32 more
core   +2 more sources

Multiple-sensor integration for efficient reverse engineering of geometry [PDF]

, 2013
This paper describes a multi-sensor measuring system for reverse engineering applications. A sphere-plate artefact is developed for data unification of the hybrid system.
Longstaff, Andrew P., Fletcher, Simon, Li, Feng, Myers, Alan   +3 more
core   +3 more sources