Results 11 to 20 of about 18,427,402 (231)

A Reverse Genetics System for Cypovirus Based on a Bacmid Expressing T7 RNA Polymerase

Viruses, 2019
Dendrolimus punctatus cypovirus (DpCPV), belonging to the genus Cypovirus within the family Reoviridae, is considered the most destructive pest of pine forests worldwide. DpCPV has a genome consisting of 10 linear double-stranded RNA segments.
Gaobo Zhang, Jian Yang, Fujun Qin, Congrui Xu, Jia Wang, Chengfeng Lei, Jia Hu, Xiulian Sun   +7 more
doaj   +4 more sources

Human Rotavirus Reverse Genetics Systems to Study Viral Replication and Pathogenesis

Viruses, 2021
Human rotaviruses (HuRVAs) are highly important causes of acute gastroenteritis in infants and young children worldwide. A lack of reliable and reproducible reverse genetics systems for HuRVAs has limited a proper understanding of HuRVA biology and also ...
Satoshi Komoto, Saori Fukuda, Takayuki Murata, Koki Taniguchi   +3 more
doaj   +2 more sources

Establishment of a Simple and Efficient Reverse Genetics System for Canine Adenoviruses Using Bacterial Artificial Chromosomes

Viruses, 2020
Canine adenoviruses (CAdVs) are divided into pathotypes CAdV1 and CAdV2, which cause infectious hepatitis and laryngotracheitis in canid animals, respectively.
Hiromichi Matsugo, Tomoya Kobayashi-Kitamura, Haruhiko Kamiki, Hiroho Ishida, Akiko Takenaka-Uema, Shin Murakami, Taisuke Horimoto   +6 more
doaj   +2 more sources

Application of the CPER reverse genetics system for genetic engineering of rabies virus [PDF]

Journal of Virology
Reverse genetics (RG) systems are essential tools for basic virological studies and applied studies using engineered recombinant viruses in various research fields.
Yukari Itakura, Nijiho Kawaguchi, Koshiro Tabata, Gabriel Gonzalez, Kei Konishi, Aiko Ohnuma, Itsuko Furuta, Naoto Ito, Shinji Saito, William W. Hall, Yasuko Orba, Hirofumi Sawa, Michihito Sasaki   +12 more
doaj   +2 more sources

A rapid yeast-based reverse genetics system reveals SARS-CoV-2 Omicron BA.2.86 variant spreads faster than Omicron JN.1 variant in primary human nasal and bronchial epithelial airway cultures [PDF]

mBio
Since the COVID-19 pandemic, several reverse genetics platforms for SARS-CoV-2 have been established. In general, a plasmid-based reverse genetics system is stable and easy to manipulate, distribute, and store.
Jiayu Xu, Michelle Chamblee, Cheng Chih Hsu, Fei Jiang, Phylip Chen, Yuexiu Zhang, Xueya Liang, Amal O. Amer, Prosper N. Boyaka, Estelle Cormet-Boyaka, Mark E. Peeples, Jianrong Li   +11 more
doaj   +2 more sources

Construction of Reverse Genetics System for Feline Calicivirus FCV‐BJ616 and Proteomic Analysis [PDF]

MicrobiologyOpen
Feline calicivirus (FCV) is a primary cause of upper respiratory tract infections and oral ulcerative disease in cats and exhibits substantial genetic diversity that complicates prevention and control.
Chunmei Xu, Jingjie Zhao, Hao Liu, Haotian Gu, Xinming Tang, Lin Liang, Jiabo Ding, Shaohua Hou, Xiaomin Zhao, Ruiying Liang   +9 more
doaj   +2 more sources

Construction and characterization of a reverse genetics system of bovine parainfluenza virus type 3c as a tool for rapid screening of antivirals in vitro [PDF]

Frontiers in Veterinary Science
Bovine parainfluenza virus type 3 (BPIV3) is a key pathogen associated with bovine respiratory disease complex (BRDC). However, its specific pathogenesis mechanisms have not been fully elucidated.
Yu Han, Kejia Lu, Riteng Zhang, Xi Wei, Hanwei Guo, Lina Tong, Lina Tong, Xinglong Wang, Sa Xiao, Haijin Liu, Zengqi Yang   +10 more
doaj   +2 more sources

Development of a reverse genetics system for Getah virus and characterization of rescued strains [PDF]

Veterinary Research
Getah virus (GETV), a neglected and re-emerging mosquito-borne alphavirus, has become more serious and poses a potential threat to animal safety and public health.
Rongxuan Cai, Qi He, Qing Wang, Lan Tian, Zhe Chen, XiaoFeng Wu, Jiumeng Sun, Ying Shao, Xiangjun Song, Kezong Qi, Jian Tu, Zhenyu Wang   +11 more
doaj   +2 more sources

What makes a weed a weed? How virus-mediated reverse genetics can help to explore the genetics of weediness [PDF]

, 2020
Reverse genetics investigates what a gene does by testing how the plant responds when the specific gene is changed. These techniques have been in use for decades to assess whether a given gene underpins interesting phenotypes and gain insight into the ...
Macgregor, D.
core   +1 more source

Development of a PCR-Based Reverse Genetics System for an Attenuated Duck Tembusu Virus Strain. [PDF]

PLoS ONE, 2016
The infectious disease caused by the duck Tembusu virus (DTMUV) has resulted in massive economic losses to the Chinese duck industry in China since 2010.
Xiaogang Wu, Ying Shi, Dawei Yan, Xuesong Li, Pixi Yan, Xuyuan Gao, Yuee Zhang, Lei Yu, Chaochao Ren, Guoxin Li, Liping Yan, Qiaoyang Teng, Zejun Li   +12 more
doaj   +1 more source