Results 61 to 70 of about 18,427,402 (231)
Efficient and Robust
mSphere, 2017 The notoriously low efficiency of Paramyxoviridae reverse genetics systems has posed a limiting barrier to the study of viruses in this family. Previous approaches to reverse genetics have utilized a wide variety of techniques to overcome the technical ...
Shannon M. Beaty +9 more
doaj +1 more source
FEBS Letters, EarlyView.This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley +1 more source
From mice to humans—divergent strategies for intestinal homeostasis and regeneration
FEBS Letters, EarlyView.Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa +2 more
wiley +1 more source
Modelling stem cell differentiation related processes—A practical overview for biologists
FEBS Letters, EarlyView.Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar +4 more
wiley +1 more source
‘REVERSE GENETICS’ IN PETUNIA HYBRIDA
, 2001 Petunia hybrida is a suitable plant system for reverse genetics ...
ZENONI, Sara +5 more
core +1 more source
Analysing Reverse Engineering Techniques for Interactive Systems [PDF]
, 2012 Reverse engineering is the process of discovering a model of a software system by analyzing its structure and functions. Reverse engineering techniques applied to interactive software applications (e.g.
Lin, Feifei (Amy)
core
Design and analysis strategies for robust microbiome ageing research
FEBS Letters, EarlyView.The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik +5 more
wiley +1 more source
Rapid generation of HCoV-229E and HCoV-OC43 reporter viruses and replicons for antiviral research
Frontiers in Cellular and Infection MicrobiologyIntroductionThe large size of coronavirus genome, along with the instability of certain genomic sequences, makes the construction of reverse genetics for coronaviruses particularly challenging.
Yuyuan Zhang +10 more
doaj +1 more source
Rapid Recovery of Classical Swine Fever Virus Directly from Cloned cDNA
Journal of Integrative Agriculture, 2013 The reverse genetics for classical swine fever virus (CSFV) is currently based on the transfection of in vitro transcribed RNA from a viral genomic cDNA clone, which is inefficient and time-consuming.
Jun-hua HUANG +6 more
doaj +1 more source
Reverse Food Logistics during the Product Life Cycle [PDF]
, 2014 This study aimed to examine reverse logistics across the food product life cycle. Based on a literature review of reverse logistics factors in food industry a set of measures were derived.
Vlachos, IP, Vlachos, Ilias
core +1 more source