Results 71 to 80 of about 18,427,402 (231)

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

Generation of rescued Japanese encephalitis virus genotype 1 from infectious full-size clone using reverse genetics

Heliyon
Japanese encephalitis virus (JEV) is a pathogen responsible for high mortality and morbidity rates among children with encephalitis. Since JEV genotype 1 (GI) is the most prevalent strain in South Korea these days, corresponding research and vaccine ...
Sehyun Kim, Min Sun Kim, Aleksandra Nowakowska, Heejae Choi, Hee Won Bang, Young Bong Kim, Hee-Jung Lee   +6 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Demonstration of a TAR-based coronavirus reverse genetics system for the generation of recombinant viruses [PDF]

, 2022
Coronaviruses (CoVs), like all viruses, are obligate intracellular parasites, meaning they rely on host molecular machinery (e.g., ribosomes), resources (e.g., nucleotides, amino acids, NTPs), and cellular environment in order to produce progeny.
Wilson, Natalie R, Smith, Everett Clinton, Harris, Debreiona Y, Sterling, Cade   +3 more
core   +1 more source

Reverse Genetics of Filoviruses

, 2017
Reverse genetics systems are used for the generation of recombinant viruses. For filoviruses, this technology has been available for more than 15 years and has been used to investigate questions regarding the molecular biology, pathogenicity, and host ...
Brandt, Janine, Finch, C., Yíngyún Caì, Courtney Finch, Jens H. Kuhn, Thomas Hoenen, Janine Brandt, Kuhn, J. H., Hoenen, Thomas, Caì, Y.   +9 more
core   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Integrated Tactile-Optical Coordinate System for the Reverse Engineering of Complex Geometry [PDF]

, 2013
To meet the requirement of both high speed and high accuracy 3D measurement for reverse engineering of artefacts, an integrated contact–optical coordinate measuring system is proposed in this paper. It combines the accuracy of contact measurement using a
Longstaff, Andrew P., Fletcher, Simon, Li, Feng, Myers, Alan   +3 more
core   +3 more sources

High-Fidelity and Cost-Effective Engineering of SARS-CoV-2

Viruses
Efficient reverse genetics systems are essential for understanding SARS-CoV-2 pathogenesis, host–virus interactions, and potential therapeutic interventions. Here, we developed a cost-effective PCR-based reverse genetics platform that splits the SARS-CoV-
Marco Olguin-Nava, Thomas Hennig, Charlene Börtlein, Patrick Bohn, Uddhav B. Ambi, Alexander Gabel, Lina M. Günter, Anne-Sophie Gribling-Burrer, Nora Schmidt, Neva Caliskan, Lars Dölken, Mathias Munschauer, Redmond P. Smyth   +12 more
doaj   +1 more source

Investigating Strategies to Support Reverse-Engineering of Interactive Systems [PDF]

, 2013
Most software applications today provide a graphical user interface (GUI), which facilitates the use of the software by offering graphical and visual elements to the users.
Alsharif, Aman
core  

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source