In vitro and ex vivo inhibition of human telomerase by anti-HIV nucleoside reverse transcriptase inhibitors (NRTIs) but not by non-NRTIs. [PDF]
PLoS ONE, 2012 Telomerase is a specialized reverse transcriptase responsible for the de novo synthesis of telomeric DNA repeats. In addition to its established reverse transcriptase and terminal transferase activities, recent reports have revealed unexpected cellular ...
Kyle R Hukezalie +3 more
doaj +1 more source
Evolution of a Reverse Transcriptase to Map N1-Methyladenosine in Human mRNA
Nature Methods, 2019 Chemical modifications to messenger RNA are increasingly recognized as a critical regulatory layer in the flow of genetic information, but quantitative tools to monitor RNA modifications in a whole-transcriptome and site-specific manner are lacking. Here
Huiqing Zhou +8 more
semanticscholar +1 more source
Protein expression from unintegrated HIV-1 DNA introduces bias in primary in vitro post-integration latency models [PDF]
, 2016 To understand the persistence of latently HIV-1 infected cells in virally suppressed infected patients, a number of in vitro models of HIV latency have been developed.
Bonczkowski, Pawel +7 more
core +1 more source
Experience With Pretravel Testing for SARS-CoV-2 at an Academic Medical Center
Academic Pathology, 2021 International travel has been a significant factor in the coronavirus disease 2019 pandemic. Many countries and airlines have implemented travel restrictions to limit the spread of the causative agent, severe acute respiratory syndrome coronavirus-2.
Katherine L. Imborek MD +5 more
doaj +1 more source
The Journey of HIV-1 Non-Nucleoside Reverse Transcriptase Inhibitors (NNRTIs) from Lab to Clinic.
Journal of Medicinal Chemistry, 2018 Human immunodeficiency virus (HIV) infection is now pandemic. Targeting HIV-1 reverse transcriptase (HIV-1 RT) has been considered as one of the most successful targets for the development of anti-HIV treatment.
V. Namasivayam +7 more
semanticscholar +1 more source
DGR mutagenic transposition occurs via hypermutagenic reverse transcription primed by nicked template RNA. [PDF]
, 2017 Diversity-generating retroelements (DGRs) are molecular evolution machines that facilitate microbial adaptation to environmental changes. Hypervariation occurs via a mutagenic retrotransposition process from a template repeat (TR) to a variable repeat ...
Guo, Huatao +6 more
core +3 more sources
Base modifications affecting RNA polymerase and reverse transcriptase fidelity
Nucleic Acids Research, 2018 Ribonucleic acid (RNA) is capable of hosting a variety of chemically diverse modifications, in both naturally-occurring post-transcriptional modifications and artificial chemical modifications used to expand the functionality of RNA. However, few studies
V. Potapov +5 more
semanticscholar +1 more source
Nature Communications, 2021 Initiation of HIV-1 reverse transcription occurs at the host tRNALys 3, which forms a complex with the 5’ end of the HIV-1 viral RNA and reverse transcriptase (RT).
Betty Ha +7 more
doaj +1 more source
Journal of Nepal Medical Association, 2021 Introduction: The emergence of severe acute respiratory syndrome coronavirus-2 pandemic is critically challenging the whole world. The real-time reverse transcriptase-polymerase chain reaction is the most widely used confirmatory test for COVID-19 ...
Narayani Maharjan +4 more
doaj +1 more source
Kinetic analysis of reverse transcriptase activity of bacterial family A DNA polymerases. [PDF]
, 2012 Some bacterial thermostable, wild-type or genetically engineered family A DNA polymerases have reverse transcriptase activity. However, difference in reverse transcriptase activities of family A DNA polymerases and retroviral reverse transcriptases (RTs)
Fujiwara, Shinsuke +4 more
core +1 more source