Results 31 to 40 of about 265,335 (347)

Expanding molecular diagnostic capacity for COVID-19 in Ethiopia: operational implications, challenges and lessons learnt

The Pan African Medical Journal, 2021
Efforts towards slowing down coronavirus (COVID-19) transmission and reducing mortality have focused on timely case detection, isolation and treatment.
Adisu Kebede, Betty Lanyero, Berhane Beyene, Mayur Lalji Mandalia, Daniel Melese, Feven Girmachew, Adamu Mekonnen, Gonfa Ayana, Nebiyou Yemanebirhan, Getnet Hailu, Habtamu Asrat, Negash Nurahmed, Andargachew Gashu, Kirubel Eshetu, Zewdu Assefa, Aschalew Abayneh, Emmanuel Musa, Ebba Abate   +17 more
doaj   +1 more source

A semi-quantitative RT-PCR method to measure the in vivo effect of dietary conjugated linoleic acid on porcine muscle PPAR gene expression

Biological Procedures Online, 2003
Conjugated linoleic acid (CLA) can activate (in vitro) the nuclear transcription factors known as the peroxisome proliferators activated receptors (PPAR).
Meadus W.J.
doaj   +1 more source

Capacity of rTth polymerase to detect RNA in the presence of various inhibitors. [PDF]

PLoS ONE, 2018
The full potential of the real-time reverse transcription polymerase chain reaction (RT-PCR) as a rapid and accurate diagnostic method is limited by DNA polymerase inhibitors as well as reverse transcriptase inhibitors which are ubiquitous in clinical ...
Dongyang Cai, Ole Behrmann, Frank Hufert, Gregory Dame, Gerald Urban   +4 more
doaj   +1 more source

The polymerase chain reaction-based SARS-CoV-2 detection

Universidad Médica Pinareña, 2020
Introduction: the reverse transcriptase polymerase chain reaction is a highly precise technique in the detection and amplification of genetic material, and therefore in the detection of viruses.
José Francisco Cancino-Mesa, Adrián Alejandro Vitón-Castillo, Jorge Casí-Torres   +2 more
doaj   +2 more sources

Fetal Cytokine Expression In UteroDetected by Reverse Transcriptase Polymerase Chain Reaction [PDF]

Pediatric Research, 1995
Cytokine expression at the maternal-fetal interface is well documented. Some authors have postulated the existence of a bidirectional cytokine signaling mechanism that is critical to the maintenance of normal pregnancy. The role of the fetus (versus fetally derived placental tissues) in this process is unknown.
Liangping Deng, Stanley M. Berry, Heide T. Moore, James N. Jarvis, Roberto Romero   +4 more
openaire   +3 more sources

Stabilized reverse transcriptase fusion proteins [PDF]

, 2016
Stabilized reverse transcriptase fusion proteins including a thermostable reverse transcriptase connected to a stabilizer protein are described. Attaching the stabilizer protein to the thermostable reverse transcriptase stabilizes the fusion protein and ...
Alan M. Lambowitz, Eman Ghanem, Georg Mohr, Sabine Mohr   +3 more
core   +1 more source

Comprehensive analysis of the circular RNA expression profile and circRNA–miRNA–mRNA network in the goat skin with divergent wool curvature

Animal Research and One Health, EarlyView., 2023
In our study, we conducted circRNA expression profiling of Zhongwei goat skin exhibiting divergent curvature wool phenotypes at two developmental stages using the RNA‐seq. Functional verification assay demonstrated that circRNA8782 regulated fibroblast proliferation.
Yingxiao Su, Zhanqiang Zhao, Zhanfa Liu, Xiaobo Li, Qian Chen, Yabin Pu, Lin Jiang, Xiaohong He, Yuehui Ma, Qianjun Zhao   +9 more
wiley   +1 more source

Quantitative Reverse Transcriptase Polymerase Chain Reaction

, 2007
Since the first documentation of real-time polymerase chain reaction (PCR),(1) it has been used for an increasing and diverse number of applications, including mRNA expression studies, DNA copy number measurements in genomic or viral DNAs,(2–7) allelic discrimination assays,(8,9) expression analysis of specific splice variants of genes(10–13) and gene ...
Prabhabhai S. Patel, Lyndon M. Gommersall, Hitendra R.H. Patel, Hitendra R.H. Patel, Manit Arya   +4 more
openaire   +2 more sources

Early changes in rat diaphragm biology with mechanical ventilation [PDF]

, 2003
To better characterize the effects of 24-hour mechanical ventilation on diaphragm, the expression of myogenic transcription factors, myosin heavy chains, and sarcoplasmic/endoplasmic reticulum calcium-ATPase pumps was examined in rats.
Cadot, Pascal, De Paepe, Kristel, Decramer, Marc, Gayan-Ramirez, Ghislaine, Rácz, Gábor Z., Testelmans, Dries, Wuytack, Frank, Zádor, Ernő   +7 more
core   +1 more source

Comparative analysis and identification of spike mutations in Iranian COVID-19 samples from the first three waves of disease

Advanced Biomedical Research, 2023
Background: The spike surface glycoprotein of SARS-CoV-2 is the essential protein in virus attachment to the target cell and cell entrance. As this protein contains immunodominant epitopes and is the main target for immune recognition, it is the critical
Parisa Shoaei, Mohammad M Ranjbar, Samaneh Tokhanbigli, Behrouz Ataei, Abbas Alibakhshi, Shaghayegh Haghjooy Javanmard, Shahrzad Ahangarzadeh   +6 more
doaj   +1 more source