Results 51 to 60 of about 973,723 (161)

HIV-1 capsid and viral DNA integration

mBio
The human immunodeficiency virus type 1 (HIV-1) genome encodes 15 proteins that perform structural, enzymatic, regulatory, and accessory functions. The capsid protein (CA) is the primary structural protein of HIV-1 and plays multiple functions during ...
Richa Dwivedi, Prem Prakash, Bajarang Vasant Kumbhar, Muthukumar Balasubramaniam, Chandravanu Dash   +4 more
doaj   +1 more source

Detection of dengue virus serotype 3 by reverse transcription-polymerase chain reaction in Aedes aegypti (Diptera, Culicidae) captured in Manaus, Amazonas

Memorias do Instituto Oswaldo Cruz, 2005
The detection of dengue virus serotypes from Aedes aegypti in Manaus, state of Amazonas was carried out using the reverse transcription-polymerase chain reaction technique.
Valéria CS Pinheiro, Wanderli P Tadei, Patrícia MSS Barros, Pedro FC Vasconcelos, Ana Cecília R Cruz   +4 more
doaj   +1 more source

Comparison of miR-106b, miR-191, and miR-30d expression dynamics in milk with regard to its composition in Holstein and Ayrshire cows [PDF]

Animal Bioscience
Objective Milk composition varies considerably and depends on paratypical, genetic, and epigenetic factors. MiRNAs belong to the class of small non-coding RNAs; they are one of the key tools of epigenetic control because of their ability to regulate gene
Marina V. Pozovnikova, Viktoria B. Leibova, Olga V. Tulinova, Elena A. Romanova, Artem P. Dysin, Natalia V. Dementieva, Anastasiia I. Azovtseva, Sergey E. Sedykh   +7 more
doaj   +1 more source

Rapid Detection of Measles Virus Using Reverse Transcriptase/Recombinase Polymerase Amplification Coupled with CRISPR/Cas12a and a Lateral Flow Detection: A Proof-of-Concept Study

Diagnostics
The measles virus is highly contagious, and efforts to simplify its diagnosis are essential. A reverse transcriptase/recombinase polymerase amplification assay coupled with CRISPR/Cas12a and an immunochromatographic lateral flow detection (RT-RPA-CRISPR ...
Elena Pinchon, Steven Henry, Fanny Leon, Chantal Fournier-Wirth, Vincent Foulongne, Jean-François Cantaloube   +5 more
doaj   +1 more source

Enhancing the reverse transcriptase function in Taq polymerase via AI-driven multiparametric rational design

Frontiers in Bioengineering and Biotechnology
IntroductionModification of natural enzymes to introduce new properties and enhance existing ones is a central challenge in bioengineering. This study is focused on the development of Taq polymerase mutants that show enhanced reverse transcriptase (RTase)
Yulia E. Tomilova, Nikolay E. Russkikh, Igor M. Yi, Elizaveta V. Shaburova, Viktor N. Tomilov, Galina B. Pyrinova, Svetlana O. Brezhneva, Olga S. Tikhonyuk, Nadezhda S. Gololobova, Dmitriy V. Popichenko, Maxim O. Arkhipov, Leonid O. Bryzgalov, Evgeniy V. Brenner, Anastasia A. Artyukh, Dmitry N. Shtokalo, Dmitry N. Shtokalo, Dmitry N. Shtokalo, Denis V. Antonets, Mikhail K. Ivanov, Mikhail K. Ivanov   +19 more
doaj   +1 more source

Reverse transcription PCR v1

, 2018

openaire   +1 more source

Identification of Introns by Reverse-Transcription PCR

BioTechniques, 1996
Hannah Alexander, Stephen Alexander
doaj   +1 more source

Ligase-Catalyzed Transcription and Reverse Transcription of XNA by T3 DNA Ligase. [PDF]

Curr Protoc
Khamissi N, Rustico L, Hili R.
europepmc   +1 more source

Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication. [PDF]

FEMS Microbiol Rev
Jehad MA, Ali LM, Pillai VN, Prabhu SG, Mustafa F, Rizvi TA.   +5 more
europepmc   +1 more source

Portable Molecular Diagnostics for Cetacean Morbillivirus: Development of a Reverse Transcription Insulated Isothermal PCR (RT-iiPCR) for Global Surveillance. [PDF]

Transbound Emerg Dis
Lattao C, Hsieh CC, Obusan MCM, Fernández A, West K, Groch KR, Catão-Dias JL, Shen KN, Yang WC.   +8 more
europepmc   +1 more source