Results 251 to 260 of about 348,380 (305)

Reverse Transcription–Polymerase Chain Reaction

Cold Spring Harbor Protocols, 2014
Reverse transcription coupled to the polymerase chain reaction (RT–PCR) is commonly used to detect the presence of mRNAs, pre-mRNAs, or other types of RNA such as noncoding RNAs. The method involves using a primer annealed to the RNA of interest. For mRNA, the primer is usually a synthetic oligo(dT)15–18, a random hexamer mixture (dN)6, or a synthetic ...
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cDNA Equalization for Reverse Transcription-Polymerase Chain Reaction Quantitation

Analytical Biochemistry, 1993
Reverse transcription coupled with the polymerase chain reaction has been increasingly utilized to study gene expression. However, most previously published quantitative techniques are limited by accurate initial RNA quantitation and do not account well for the relative efficiency of reverse transcription.
J, Kolls, P, Deininger, J C, Cohen, J, Larson   +3 more
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Reverse Transcription‐Polymerase Chain Reaction‐Based Detection of Plant Viruses

Current Protocols in Microbiology, 2007
AbstractA one‐step reverse transcription–polymerase chain reaction (RT‐PCR) is used to detect two cherry flexiviruses, Cherry green ring mottle virus (CGRMV) and Cherry necrotic rusty mottle virus (CNRMV), in Prunus species. This unit presents procedures for collection of plant samples, preparation of total nucleic acids, viral RNA‐rich or total RNA ...
Ruhui, Li, John S, Hartung
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Mapping Plastid Transcript Population by Circular Reverse Transcription Polymerase Chain Reaction

2018
During evolution of photosynthetic organisms, the genetic information provided by the internalized cyanobacteria has been transferred to the nucleus. The small genome kept by the chloroplast, the so-called plastome, displays a complex organization, comprising operons under the control of multiples promoters.
Courtois, Florence, Merendino Iseni, Livia   +1 more
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Analysis of Gene Expression: Reverse Transcription-Polymerase Chain Reaction

2003
Numerous techniques have been developed to measure gene expression in tissues and cells. These include coupled reverse transcription and polymerase chain reaction amplification (RT-PCR), Northern blot (see Chapter 20 ), in situ hybridization (see Chapter 21 ), RNase protection assays, dot blots, and S1 nuclease assays.
el-B, Haddad, J, Rousell
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Quantification of RNA damage by reverse transcription polymerase chain reactions

Analytical Biochemistry, 2006
RNA damages, such as those generated by nucleic acid-modifying agents, occur randomly in RNA and present challenging problems to organisms. It has been unclear how RNA function would be affected by many forms of RNA damage and how cells are protected against the damage.
Xin, Gong, Rui, Tao, Zhongwei, Li
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Integrated reverse transcription polymerase chain reaction systems for virus detection

Biosensors and Bioelectronics, 2007
The current study reports on an integrated microreverse transcription polymerase chain reaction (RT-PCR) system for molecular diagnosis of microorganisms automatically. By using antibodies-conjugated superparamagnetic beads, the developed system can detect viruses with higher sensitivity and specificity when compared with traditional biological ...
Kang-Yi, Lien, Wan-Chi, Lee, Huan-Yao, Lei, Gwo-Bin, Lee   +3 more
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Polymerase Chain Reaction and Reverse Transcription-Polymerase Chain Reaction

2009
Polymerase chain reaction (PCR) enables one to determine if a specific needle is present in a haystack, and it can be used as a step toward the characterization of the needle. It is a quick, powerful, inexpensive DNA amplification technique that has become a fundamental tool in molecular pathology.
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Pitfalls of reverse transcription quantitative polymerase chain reaction standardization: Volume-related inhibitors of reverse transcription

Analytical Biochemistry, 2011
A large part of the reliability of reverse transcription quantitative polymerase chain reaction (RT-qPCR) data depends on technical variations. Such variations are mainly attributable to the reverse transcription step. Standardization is a key factor in decreasing the intersample variability.
Pascal, Pugniere, Sebastien, Banzet, Thomas, Chaillou, Catherine, Mouret, Andre, Peinnequin   +4 more
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