Results 261 to 270 of about 348,380 (305)

Reverse Transcription Polymerase Chain Reaction

2019
The polymerase chain reaction (PCR) technique was developed to amplify, with high efficiency, double-stranded or single-stranded deoxyribonucleic acid (DNA) sequences. Amplification of individual Ribonucleic acid (RNA) molecules can also be accomplished with PCR when coupled with reverse transcription (RT) of RNA into a complementary DNA (cDNA) copy. A
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Competitive reverse transcription-polymerase chain reaction for quantifying murine AIDS virus

Journal of Virological Methods, 1996
The causative agent of murine AIDS (MAIDS) is the defective murine leukemia virus BM5d, that requires the replication-competent ecotropic MuLV (BM5e) helper virus. We developed a competitive quantitative PCR method including specific internal standards to quantify the expression of BM5d in the spleen of infected mice and to characterize BM5d expression
B, Desforges, I, Hatin, K, Akarid, M, Sinet   +3 more
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Semiquantitative reverse transcription-polymerase chain reaction with the Agilent 2100 Bioanalyzer

ELECTROPHORESIS, 2001
We have applied a method to monitor mRNA expression in a semiquantitative fashion on the Agilent 2100 Bioanalyzer. The method was originally described in 1994 by Wong et al. and referred to as the "primer-dropping" method. This polymerase chain reaction (PCR) technique uses multiple sets of primer pairs in a coamplification reaction that amplifies the ...
Gottwald, E., Müller, O., Polten, A.
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Bacterial DNA decontamination for reverse transcription polymerase chain reaction (RT-PCR)

Journal of Microbiological Methods, 2002
For RT-PCR, removal of contaminating genomic DNA in RNA samples using manganese sulfate was more effective than magnesium. DNA contamination was removed in 3 microg of nucleic acid using 10 U of RNase-free DNase I in 10-microl reaction volumes. The digestion procedure was compatible with commercial RNA extraction kits and was suitable for RT-PCR assay.
Gehua, Wang, Connie, Barton, Frank G, Rodgers   +2 more
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Detection of Salmonella enteritidis by reverse transcription-polymerase chain reaction (PCR)

International Journal of Food Microbiology, 1999
A reverse transcription-polymerase chain reaction (RT-PCR) method was developed for detecting mRNA from the sefA gene of Salmonella enteritidis. Detection of target mRNA was examined from cells grown in buffered peptone water at different temperatures (37, 25 and 15 degrees C) and pH (5.5, 7.2 and 8.5).
E A, Szabo, B M, Mackey
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Clinical Application of Reverse Transcription-Polymerase Chain Reaction for HIV Infection

Clinics in Laboratory Medicine, 1994
This article describes the current knowledge of the various RT-PCR assays and their clinical application in HIV disease. The importance of assay precision and sample handling is emphasized. A review of quantification techniques is also presented. Finally, the relevant literature describing the application of RT-PCR for both virion RNA in plasma and ...
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[16S rRNA reverse transcription-polymerase chain reaction of leptospires].

Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao, 1995
We amplified the leptospiral RNAs of Leptospira interrogans serovar lai strain Lai and L. biflexa serovar patoc strain Patoc I, extracted by the method of SiO2-high concentration salt solution (8 mol/L GuHCl) absorption, with the 16S rRNA gene primers of Leptospira interrogans by reverse transcription-polymerase chain reaction (RT-PCR). It demonstrated
W, Wu, B, Dai
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Relative Reverse Transcription-Polymerase Chain Reaction

2003
T A, Giambernardi, R J, Klebe
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The assembly, regulation and function of the mitochondrial respiratory chain

Nature Reviews Molecular Cell Biology, 2021
Irene Vercellino, Leonid A Sazanov
exaly  

Upcycling CO2 into energy-rich long-chain compounds via electrochemical and metabolic engineering

Nature Catalysis, 2022
Chunxiao Liu, Xu Li, Qiu Jiang
exaly