Results 281 to 290 of about 677,547 (352)

Immunoregulatory Roles of Tumor‐Originated Pericytes Identified by Single‐Cell Analysis in Glioblastoma

Advanced Science, EarlyView.
The origin and functional heterogeneity of pericytes in glioblastoma (GBM) remain unclear. This study identifies tumor‐originated pericytes (T‐PCs) and normal‐originated pericytes as two distinctive populations in human GBM using single‐cell RNA‐sequencing.
Cuiying Chu, Fangzhen Li, Zhiwen Zhang, Qiuhong Zhu, Hong Yan, Mengdan Cheng, Huipeng Wang, Lei Cheng, Zhe Zhang, Xingjiang Yu, Jianghong Man, Wei Wang, Dongxue Li, Xiu‐wu Bian, Hai‐bo Wu, Aili Zhang, Wenchao Zhou   +16 more
wiley   +1 more source

Soybean Auxin Transporter PIN3 Regulates Nitrate Acquisition to Improve Nitrogen Use and Seed Traits

Advanced Science, EarlyView.
Xu et al. discovers that switching off auxin transporters in soybean, PIN3a and PIN3b, disrupts auxin flow; this triggers ARF‐STF3/4 signal cascade to activate the nitrate importer NPF2.13 and soil nitrogen acquisition. Multi‐year field trials show edited soybeans maintain yield with higher oil content, offering a potential genetic route to improve ...
Huifang Xu, Shiyu Huang, Jie Wang, Tian Wang, Qingqing Han, Kexin Wu, Zhen Gao, Xiaolei Shi, Tianli Tu, Ming Wang, Laimei Huang, Jiaomei Chen, Yunqi Liu, Yumei Zhang, Guoqiang Lin, Zhichang Chen, Xu Chen   +16 more
wiley   +1 more source

Detection of the (11;22)(q24;q12) translocation of Ewing's sarcoma and peripheral neuroectodermal tumor by reverse transcription polymerase chain reaction.

, 1993
JR Downing, David R. Head, David M. Parham, E C Douglass, M G Hulshof, M P Link, T A Motroni, Holcombe E Grier, AM Curcio-Brint, David N. Shapiro   +9 more
openalex   +1 more source

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Reverse transcription-polymerase chain reaction.

Cold Spring Harbor Protocols, 2014
Reverse transcription coupled to the polymerase chain reaction (RT–PCR) is commonly used to detect the presence of mRNAs, pre-mRNAs, or other types of RNA such as noncoding RNAs. The method involves using a primer annealed to the RNA of interest. For mRNA, the primer is usually a synthetic oligo(dT)15–18, a random hexamer mixture (dN)6, or a synthetic ...
D. Rio
semanticscholar   +3 more sources

Reverse Transcription Polymerase Chain Reaction

Neuroscience Methods, 2019
The polymerase chain reaction (PCR) technique was developed to amplify, with high efficiency, double-stranded or single-stranded deoxyribonucleic acid (DNA) sequences. Amplification of individual Ribonucleic acid (RNA) molecules can also be accomplished with PCR when coupled with reverse transcription (RT) of RNA into a complementary DNA (cDNA) copy. A
M. Vidovic
semanticscholar   +2 more sources

Quantification of RNA by Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR).

Cold Spring Harbor Protocols, 2018
This protocol describes a real-time reverse transcription-polymerase chain reaction (RT-PCR) assay using a two-enzyme, two-tube approach, carried out using either SYBR Green I or TaqMan chemistries.
Michael R Green, J. Sambrook
semanticscholar   +1 more source

Quantification of RNA damage by reverse transcription polymerase chain reactions

Analytical Biochemistry, 2006
RNA damages, such as those generated by nucleic acid-modifying agents, occur randomly in RNA and present challenging problems to organisms. It has been unclear how RNA function would be affected by many forms of RNA damage and how cells are protected against the damage.
Xin, Gong, Rui, Tao, Zhongwei, Li
openaire   +2 more sources

cDNA Equalization for Reverse Transcription-Polymerase Chain Reaction Quantitation

Analytical Biochemistry, 1993
Reverse transcription coupled with the polymerase chain reaction has been increasingly utilized to study gene expression. However, most previously published quantitative techniques are limited by accurate initial RNA quantitation and do not account well for the relative efficiency of reverse transcription.
J, Kolls, P, Deininger, J C, Cohen, J, Larson   +3 more
openaire   +2 more sources

Analysis of Gene Expression: Reverse Transcription-Polymerase Chain Reaction

2003
Numerous techniques have been developed to measure gene expression in tissues and cells. These include coupled reverse transcription and polymerase chain reaction amplification (RT-PCR), Northern blot (see Chapter 20 ), in situ hybridization (see Chapter 21 ), RNase protection assays, dot blots, and S1 nuclease assays.
el-B, Haddad, J, Rousell
openaire   +2 more sources