Results 301 to 310 of about 677,547 (352)

Quantitative reverse transcription polymerase chain reaction of a molluscan metallothionein mRNA

Aquatic Toxicology, 2001
A quantitative assay based on competitive reverse transcription polymerase chain reaction (RT-PCR) was developed for metallothionein (MT) mRNA of the mollusc Crassostrea virginica and applied to analysis of MT mRNA of hemocytes. The assay was based on titration of a competitive external standard cRNA derived from the coding region of the oyster MT mRNA.
R A, Butler, G, Roesijadi
openaire   +2 more sources

Pitfalls of reverse transcription quantitative polymerase chain reaction standardization: Volume-related inhibitors of reverse transcription

Analytical Biochemistry, 2011
A large part of the reliability of reverse transcription quantitative polymerase chain reaction (RT-qPCR) data depends on technical variations. Such variations are mainly attributable to the reverse transcription step. Standardization is a key factor in decreasing the intersample variability.
Pascal, Pugniere, Sebastien, Banzet, Thomas, Chaillou, Catherine, Mouret, Andre, Peinnequin   +4 more
openaire   +2 more sources

Clinical Application of Reverse Transcription-Polymerase Chain Reaction for HIV Infection

Clinics in Laboratory Medicine, 1994
This article describes the current knowledge of the various RT-PCR assays and their clinical application in HIV disease. The importance of assay precision and sample handling is emphasized. A review of quantification techniques is also presented. Finally, the relevant literature describing the application of RT-PCR for both virion RNA in plasma and ...
openaire   +2 more sources

Detection of tobacco rattle virus by reverse transcription and polymerase chain reaction

Journal of Virological Methods, 1992
An assay involving reverse transcription followed by polymerase chain reaction specifically detected tobacco rattle virus RNA in extracts of infected plants. It detected a wide range of serological variants and typical non-particle producing NM-type isolates.
openaire   +2 more sources

Relative Reverse Transcription-Polymerase Chain Reaction

2003
T A, Giambernardi, R J, Klebe
openaire   +2 more sources

Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods.

Analytical Biochemistry, 2000
Thomas D. Schmittgen, B. A. Zakrajsek, A. Mills, V. Gorn, M. J. Singer, M. Reed   +5 more
semanticscholar   +1 more source

Quantification of mRNA levels with reverse transcription-polymerase chain reaction

Biochemical and Biophysical Research Communications, 2003
Johanna, Weiss, Nadine, Albermann
openaire   +2 more sources

The assembly, regulation and function of the mitochondrial respiratory chain

Nature Reviews Molecular Cell Biology, 2021
Irene Vercellino, Leonid A Sazanov
exaly  

Selection of reference genes for quantitative reverse-transcription polymerase chain reaction normalization in Brassica napus under various stress conditions

Zeitschrift für Induktive Abstammungs- und Vererbungslehre, 2014
Z. Wang, Yu Chen, Hedi Fang, Haifeng Shi, Keping Chen, Zhiyan Zhang, Xiaoli Tan   +6 more
semanticscholar   +1 more source

Introduction to Reverse Transcription Polymerase Chain Reaction

Diagnostic Molecular Pathology, 1995
openaire   +2 more sources