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Advanced Science, EarlyView.γGC deficiency in ulcerative colitis drives macrophage ferroptosis‐mediated inflammation. Claw‐engaged microparticles armed with γGC (γGC‐MPs) enhance lesion targeting, promoting localized γGC release and tissue uptake. On the cover, Monkey King (Macrophages) was bound by the Immortal‐Binding Rope and subsequently sealed beneath the Five‐Fingered ...
Rong Wang +10 more
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Advanced Science, EarlyView.Different from mitosis, the female meiosis undergoes asymmetric division that produces haploid oocytes and polar body, which is essential for retaining maternal components to support subsequent fertilization and embryo development. However, the underlying mechanisms are still largely unknown.
Yu Li +6 more
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Comparative analysis of reverse-transcription-polymerase chain reaction for Aichivirus detection [PDF]
Food Science and BiotechnologyAichivirus-A (AiV-A), a member of the Kobuvirus genus of the family Picornaviridae, was first reported in stool samples of patients with non-bacterial gastroenteritis in Aichi Prefecture, Japan, in 1989. AiV has been reported from in various aquatic environments, such as surface water and sewage, can be transmitted via the fecal-oral route through ...
Mi-Gyeong, Kim +6 more
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Diagnosis of Echinococcus multilocularis infection by reverse-transcription polymerase chain reaction [PDF]
Gastroenterology, 1995 Alveolar echinococcosis is a life-threatening parasitosis occurring in countries in the Northern hemisphere. The diagnosis of an Echinococcus multilocularis infection is routinely performed by radiological techniques and by the detection of specific antibodies in the sera of infected patients.
K. Beckh +8 more
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Quantification of RNA damage by reverse transcription polymerase chain reactions
Analytical Biochemistry, 2006RNA damages, such as those generated by nucleic acid-modifying agents, occur randomly in RNA and present challenging problems to organisms. It has been unclear how RNA function would be affected by many forms of RNA damage and how cells are protected against the damage.
Rui Tao, Xin Gong, Zhongwei Li
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Reverse Transcription–Polymerase Chain Reaction
Cold Spring Harbor Protocols, 2014Reverse transcription coupled to the polymerase chain reaction (RT–PCR) is commonly used to detect the presence of mRNAs, pre-mRNAs, or other types of RNA such as noncoding RNAs. The method involves using a primer annealed to the RNA of interest. For mRNA, the primer is usually a synthetic oligo(dT)15–18, a random hexamer mixture (dN)6, or a synthetic ...
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Polymerase Chain Reaction and Reverse Transcription-Polymerase Chain Reaction [PDF]
, 2009 Polymerase chain reaction (PCR) enables one to determine if a specific needle is present in a haystack, and it can be used as a step toward the characterization of the needle. It is a quick, powerful, inexpensive DNA amplification technique that has become a fundamental tool in molecular pathology.
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cDNA Equalization for Reverse Transcription-Polymerase Chain Reaction Quantitation
Analytical Biochemistry, 1993Reverse transcription coupled with the polymerase chain reaction has been increasingly utilized to study gene expression. However, most previously published quantitative techniques are limited by accurate initial RNA quantitation and do not account well for the relative efficiency of reverse transcription.
J.C. Cohen +3 more
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